Proteomic identification of the macrophage-capping protein as a protein contributing to the malignant features of hepatocellular carcinoma

Kimura, Kazuya; Ojima, Hidenori; Kubota, Daisuke; Sakumoto, Marimu; Nakamura, Yukiko; Tomonaga, T.; Kosuge, Tmoo; Kondo, Tadashi

doi:10.1016/j.jprot.2012.10.004

Cited by 35 publications

(33 citation statements)

References 41 publications

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“…CapG is overexpressed in lung cancer, breast cancer, pancreatic cancer, bile duct cancer, and colorectal cancer cells, as well as ovarian tumors and hepatocellular carcinoma. In addition, CapG can be utilized clinically as a part of the treatment for these malignancies (Thompson et al, 2007;Partheen et al, 2008;Zheng et al, 2010;Shao et al, 2011;Wu et al, 2011;Morofuji et al, 2012;Zhu et al, 2012;Kimura et al, 2013). Therefore, CapG has certain clinical prospects in the treatment and diagnosis of various types of cancer.…”

Section: Introductionmentioning

confidence: 99%

Influence of suppression of CapG gene expression by siRNA on the growth and metastasis of human prostate cancer cells

Li¹,

Guo²,

Li³

et al. 2015

Genet. Mol. Res.

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ABSTRACT. This study investigated CapG gene expression in prostate cancer cell lines; in addition, we explored the effects of CapG suppression on DU145 cell growth, and the underlying mechanism with which CapG affects DU145 cell growth and invasiveness. The expression of CapG and 18 related genes in DU145 cells was analyzed by flow cytometry, quantitative polymerase chain reaction (qPCR), CCK8 assay, western blot, and the trans-well assay. DU145 cells were transfected with designed small interfering RNA (siRNA). CapG expression was quantified by qPCR and western blot. DU145 cell proliferation and invasiveness was analyzed using the CCK8, flow cytometric, and trans-well assays. CapG, TMPRSS1, EGFR, ETS-1, ERBB2, AKT, Cyclin D1, P21, Bcl-2, and Bak1 gene and Bcl-2, Cyclin D1, and CapG protein expressions were significantly lower in the siRNA group compared to the negative control group (P < 0.05). The proliferation of CapG siRNA DU145 cells was lower than that of the two control groups, 48 h after transfection. The cell inhibition rate was 24.5, 35.4, and 16,5% at 24, 48, and 72 h, respectively. The growth curve indicated that CapG siRNA DU145 cells showed a significantly slower 15769-15778 (2015) proliferation rate (P < 0.05). The trans-well assay showed a significant decrease in the migratory and invasive capacities of DU145 cells in the siRNA group (P < 0.05). The suppression of CapG expression caused a significant decrease in the proliferation, invasiveness, and metastasis of DU145 cells. The mechanism with which CapG, with other oncogenes, influences cancer cell cycle remains to be elucidated.

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Section: Introductionmentioning

confidence: 99%

Influence of suppression of CapG gene expression by siRNA on the growth and metastasis of human prostate cancer cells

Li¹,

Guo²,

Li³

et al. 2015

Genet. Mol. Res.

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“…Most previous reports demonstrated a positive association between cancer and CAPG protein expression [5][6][7][8][9][10][25][26][27][28][29]. Although it has been reported that CAPG SVPs was associated with carotid atherosclerosis [30], to our best knowledge, this is the first report which examined an association of malignant tumor with CAPG polymorphism in detail.…”

Section: Discussionmentioning

confidence: 71%

Association of Macrophage Capping Protein (CAPG) Arg335 his Polymorphism and Cancer Susceptibility in the Elderly Japanese

Dechamethakun¹,

Sato²,

Ikeda³

et al. 2017

J Gerontol Geriatr Res

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“…75 Remarkably, this paper was the first to identify MCP as cancer biomarker. Other studies on different human malignancies [76][77][78][79][80] validated MCP as a cancer biomarker, further supporting the use of dogs as useful models for human cancer. Studies on the serum proteome changes of canine lymphoma have been carried out by combining ion exchange chromatography and surface-enhanced laser desorption ionisation (SELDI) mass spectrometry.…”

Section: View Article Onlinementioning

confidence: 73%

Application of post-genomic techniques in dog cancer research

et al. 2016

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Omics techniques have been widely applied to veterinary science, although mostly on farm animal productions and infectious diseases. In canine oncology, on the contrary, the use of omics methodologies is still far behind. This review presents the most recent achievement in the application of postgenomic techniques, such as transcriptomics, proteomics, and metabolomics, to canine cancer research. The protocols to recover material suitable for omics analyses from formalin-fixed, paraffinembedded tissues are presented, and omics applications for biomarker discovery and their potential for cancer diagnostics in veterinary medicine are highlighted.

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Proteomic identification of the macrophage-capping protein as a protein contributing to the malignant features of hepatocellular carcinoma

Cited by 35 publications

References 41 publications

Influence of suppression of CapG gene expression by siRNA on the growth and metastasis of human prostate cancer cells

Influence of suppression of CapG gene expression by siRNA on the growth and metastasis of human prostate cancer cells

Association of Macrophage Capping Protein (CAPG) Arg335 his Polymorphism and Cancer Susceptibility in the Elderly Japanese

Application of post-genomic techniques in dog cancer research

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