Proteomic identification of sperm antigens using serum samples from individuals with and without antisperm antibodies

Nowicka-Bauer, Karolina; Kamieniczna, Marzena; Cibulka, J; Z, Ulcová-Gallová; Kurpisz, Maciej

doi:10.1111/and.12502

Cited by 18 publications

(11 citation statements)

References 51 publications

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“…Three of these proteins (ACR, GAPDHS, and ODF1) are strictly present on spermatozoa, making them good sperm-cell-specific antigens. Among the sperm antigens identified, several are known to be involved in key steps of the fertilization process, such as HSPA1L [ 61 ], CCT3 [ 62 ], ZPBP and ZPBP2 [ 63 ], and HSPD1 [ 64 , 65 ]. Others, such as AKAP3 and ROPN1, are associated with the acrosomal reaction process [ 66 , 67 ].…”

Section: Discussionmentioning

confidence: 99%

Identification of Arvicola terrestris scherman Sperm Antigens for Immune Contraceptive Purposes

Chorfa

Goubely

Henry-Berger

et al. 2021

IJMS

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The cyclical proliferation of the wild fossorial rodent Arvicola terrestris scherman (ATS) is critical in mid-mountain ecosystems of several European countries. Our goal is to develop an immunocontraceptive vaccine to control their fertility, as a sustainable alternative to chemical poisons currently used. Indeed, these chemicals cause the death of ATS predators and animals sharing their ecosystem, and current laws progressively limit their use, making the development of a targeted vaccination strategy an interesting and efficient alternative. In order to identify species-specific sperm antigens, male and female ATS received subcutaneous injections of whole ATS spermatozoa to elicit an immune response. The analysis of the immune sera led to the identification of 120 immunogenic proteins of sperm cells. Of these, 15 were strictly sperm-specific and located in different regions of the male gamete. Some of these antigens are proteins involved in molecular events essential to the reproductive process, such as sperm–egg interaction, acrosomal reaction, or sperm motility. This approach not only identified a panel of immunogenic proteins from ATS sperm cells, but also demonstrated that some of these proteins trigger an immune response in both male and female ATS. These spermatic antigens are good candidates for the development of a contraceptive vaccine.

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Section: Discussionmentioning

confidence: 99%

Identification of Arvicola terrestris scherman Sperm Antigens for Immune Contraceptive Purposes

Chorfa

Goubely

Henry-Berger

et al. 2021

IJMS

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“…Simultaneously, PGK2 and ACRBP were found to react to seminal plasma of immunoinfertile men and sera of vasectomized patients respectively. The other research also indicated that ENO1 reacted to both antisperm antibody-positive and -negative sera, whereas ACRBP did only to the positive serum, but not to the negative [ 16 ].…”

Section: Discussionmentioning

confidence: 99%

Detection of sperm-reactive antibodies in wild sika deer and identification of the sperm antigens

Kawase

Jimbo

2018

The Journal of Veterinary Medical Science

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Antisperm antibodies potentially inhibit sperm functions causing the sterility in humans and experimentally treated animals. However, there is no information about antisperm antibodies emerging spontaneously in wildlife. In this study, we searched for the sperm-reactive antibodies, spontaneously produced in wild sika deer (Cervus nippon), and identified the sperm antigens. We collected 529 fecal masses of sika deer in Japanese cities, from which we extracted the mucosal antibodies to test them for reactivities to deer sperm proteins by ELISA. Two of the extracts contained IgAs that were highly reactive to the sperm proteins. The molecular weights of the active IgAs, partially purified by DEAE-sephadex A-50, were estimated at more than 100 kDa, suggesting that the IgAs evaded drastic digestion in the gastrointestinal tract. Two-dimensional electrophoresis and immunoblotting detected three major antigens, and the following LC-MS/MS analysis identified them as alpha-enolase, phosphoglycerate kinase 2 and acrosin-binding protein. The antibodies were cross-reactive to a recombinant human acrosin-binding protein. To our knowledge, this is the first research to find that the sperm-reactive antibodies are produced spontaneously in wildlife and they recognize a common antigen found in humans.

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“…Sera containing ASAs have been utilized to identify the relevant antibody‐mediated infertility immune dominant antigens (Shetty et al, ). These included HSP70, tubulin α 1a, class 4b β‐tubulin, ATP synthase subunit β, carbonic anhydrase 2, α‐enolase, and lactate dehydrogenase (LDHC) (Nowicka‐Bauer et al, ). Western blot analysis has been used to quantify these antigens and to identify protein–protein interactions (Auer, Senechal, Desvaux, Albert, & De Almeida, ).…”

Section: Diagnostic Methods To Identify Immune Regulatory Moleculesmentioning

confidence: 99%

“…These include T‐complex protein 1 subunit θ, arylsulfatase A, and arrestin domain‐containing protein‐5. Since these proteins are involved in spermatozoon–oocyte interaction (Nowicka‐Bauer, Kamieniczna, Cibulka, Ulcova‐Gallova, & Kurpisz, ), presence of ASAs against these proteins may result in failure of sperm‐zona binding and infertility.…”

Section: Testicular Immune Mechanismmentioning

confidence: 99%

Immune regulatory molecules as modifiers of semen and fertility: A review

Archana

Selvaraju

Binsila

et al. 2019

Molecular Reproduction Devel

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Declining fertility rates in both human and animals is a cause for concern. While many of the infertility cases are due to known causes, idiopathic infertility is reported in 30% of the infertile couples. In such cases, 18% of the infertile males carry antisperm antibodies (ASAs). Such data are lacking in livestock, wherein 20–30% of the animals are being culled due to low fertility. In males, the blood–testis barrier (BTB) and biomolecules in the semen provide an immuno‐tolerant microenvironment for spermatozoa as they traverse the immunologic milieu of both the male and female reproductive tracts. For example, insults from environmental contaminants, infections and inflammatory conditions are likely to impact the immune privilege state of the testis and fertility. The female mucosal immune system can recognize allogenic spermatozoa‐specific proteins affecting sperm kinematics and sperm‐zona binding leading to immune infertility. Elucidating the functions and pathways of the immune regulatory molecules associated with fertilization are prerequisites for understanding their impact on fertility. An insight into biomolecules associated with spermatozoal immune tolerance may generate inputs to develop diagnostic tools and modulate fertility. High‐throughput sequencing technologies coupled with bioinformatics analyses provides a path forward to define the array of molecules influencing pregnancy outcome. This review discusses the seminal immune regulatory molecules from their origin in the testis until they traverse the uterine environment enabling fertilization and embryonic development. Well‐designed experiments and the identification of biomarkers may provide a pathway to understand the finer details of reproductive immunology that will afford personalized therapies.

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Proteomic identification of sperm antigens using serum samples from individuals with and without antisperm antibodies

Cited by 18 publications

References 51 publications

Identification of Arvicola terrestris scherman Sperm Antigens for Immune Contraceptive Purposes

Identification of Arvicola terrestris scherman Sperm Antigens for Immune Contraceptive Purposes

Detection of sperm-reactive antibodies in wild sika deer and identification of the sperm antigens

Immune regulatory molecules as modifiers of semen and fertility: A review

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