Proteomic identification of rainbow trout sperm proteins

Abstract

Proteomics represents a powerful tool for the analysis of fish spermatozoa, since these cells are transcriptionally inactive. The aim of the present study was to generate an inventory of the most prominent rainbow trout sperm proteins by SDS-PAGE prefractionation combined with nano-LC-MS/MS based identification. This study provides the first in-depth analysis of the rainbow trout sperm proteome, with a total of 206 identified proteins. We found that rainbow trout spermatozoa are equipped with functionally diverse proteins related to energetic metabolism, signal transduction, protein turnover, transport, cytoskeleton, oxidative injuries, and stress and reproduction. The availability of a catalog of rainbow trout sperm proteins provides a crucial tool for the understanding of fundamental molecular processes in fish spermatozoa, for the ongoing development of novel markers of sperm quality and for the optimization of short- and long-term sperm preservation procedures. The MS data are available at ProteomeXchange with the dataset identifier PXD000355 and DOI 10.6019/PXD000355.

The Sperm Proteome of the Echiuran Urechis unicinctus (Annelida, Echiura)

“…Examples of this group of proteins are ATP synthase subunit, fumarate hydratase, and phosphoglycerate kinase. The large number of energy metabolism related proteins in sperm might be due to the high energy requirement for sperm motility, consistent with previous studies of sperm proteome . “Protein synthesis and degradation” is the second largest group (18%).…”

Protein profile of seminal plasma and functionality of spermatozoa during the reproductive season in the common carp (Cyprinus carpio) and rainbow trout (Oncorhynchus mykiss)

“…One limitation to the identification of all the proteins present in seminal plasma, however, is the completeness of the genome. For example, the number of proteins identified in carp was lower than in rainbow trout (Nynca et al, ), likely because its genome is incomplete. In the present study, the application of two‐dimensional gel electrophoresis combined with MALDI‐TOF/TOF mass spectrometry helped us identify 13 differentially expressed protein spots in carp seminal plasma.…”

“…Studies of trout sperm revealed that about 25% of the identified proteins are shared by seminal plasma and spermatozoa, including multi‐task proteins (apolipoproteins, transferrin, serum albumin); proteins related to the immune system (complements); heat‐shock proteins; antioxidant proteins (thioredoxin, superoxide dismutase, glutathione‐S‐transferase); cytoskeletal proteins (tubulin, actin); 14‐3‐3 family members; and enzymes (lactate dehydrogeanse, creatine kinase, glyceraldehyde‐3‐phosphate dehydrogenase). Multi‐task and immunity‐related proteins were previously identified as the most abundant (Nynca et al, ). The seminal plasma proteins could presumably be adsorbed on the sperm surface, thereby directly protecting spermatozoa (Belleannee et al, ).…”

Proteomic Analysis of the Lake Trout (Salvelinus namaycush) Liver Identifies Proteins from Evolutionarily Close and ‐Distant Fish Relatives

“…Methylmercury induced proteomic changes in the Atlantic cod liver revealed a total of 1143 proteins with 125 differentially regulated upon exposure . Furthermore, many studies have been performed that have helped to characterize the proteome of the species Oncorhynchus mykiss (rainbow trout) which is from the same family as the lake trout, but from a different genus, and the response of the proteome from external stimuli . This species has a very well developed protein database consisting of roughly 260 000 proteins .…”