Proteomic identification of protein associated to mature spermatozoa in the Pacific oyster Crassostrea gigas

Kingtong, Sutin; Kellner, K; Bernay, Benoı̂t; Goux, Didier; Sourdaine, Pascal; Berthelin, Clothilde Heude

doi:10.1016/j.jprot.2013.02.009

Cited by 20 publications

(35 citation statements)

References 41 publications

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“…We compared our results with the recent proteomic study which screened out 31 proteins that might function in spermatogenesis of C. gigas (Kingtong et al 2013). Several proteins we observed here are consistent with those reported previously (such as tubulin alpha-3 chain, 14-3-3 protein zeta, ATP synthase subunit alpha).…”

Section: Resultssupporting

confidence: 84%

“…(Tops et al 2010;Chauvin et al 2012;Rettie & Dorus 2012;Bayasula et al 2013;McReynolds et al 2014). In C. gigas, two preliminary proteomic studies have been conducted to identify proteins associated with oocyte quality and spermatogenesis (Corporeau et al 2012;Kingtong et al 2013). However, since these studies focus on the proteins related to particular characteristics (gamete quality or development), little attention is paid on other molecules.…”

Section: Introductionmentioning

confidence: 99%

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The sperm proteome of the Pacific oysterCrassostrea gigasand immunolocalization of heat shock proteins

Huang

Liu

Huan

2015

Invertebrate Reproduction & Development

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The Pacific oyster Crassostrea gigas is a potential model organism of bivalve mollusks. Comprehensive studies on the proteome of its sperm are necessary to expand our understanding on its reproduction and development, which however are still poor currently. In this study, to improve the situation, we conducted a proteomic analysis on the sperm based on two-dimensional electrophoresis combined with protein identification through mass spectra data. Fifty-six protein spots with constant and relatively high expression levels were selected for protein identification. Among them, 36 were identified (corresponding to 31 proteins), including cytoskeletal proteins, proteins involved in energy supply, protein modifiers, signal regulators, antioxidant proteins, and others. We proposed that these proteins might play important roles in sperm motility, gamete interaction, and oxidation resistance. In particular, we observed several heat shock proteins that were proved to play essential roles in animal sperms. A further immunofluorescence experiment revealed a mitochondria localization of Hsp60s and wide distributions of Hsp70s, indicating these proteins might function in various processes such as mitochondrial function, gamete interaction, and regulation of receptor activity. Our data will provide fundamental supports for the studies on the mechanisms of fertilization and contribute to expand the understandings on reproduction and development of bivalve mollusks.

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Section: Resultssupporting

confidence: 84%

Section: Introductionmentioning

confidence: 99%

The sperm proteome of the Pacific oysterCrassostrea gigasand immunolocalization of heat shock proteins

Huang

Liu

Huan

2015

Invertebrate Reproduction & Development

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“…Comparable to the results of our study, tektin has also been found to be down-regulated in C. gigas larvae exposed to a pH of about 7.5 for 6 days [113]. In addition, another study in C. gigas showed that two forms of tektin were present in the spermatozoa of the oyster [114]. Correspondingly, CCDC135 (coiled-coil domain-containing protein lobo homolog), a flagellar protein, has been shown to localize in the testis and along the sperm flagellum of the fly Drosophila melanogaster, with some sperm motility defects seen in the single and double mutants [115].…”

Section: Ciliary Functionsupporting

confidence: 74%

Molecular analysis of the Sydney rock oyster (Saccostrea glomerata) CO2 stress response

Ertl

O’Connor

Wiegand

et al. 2016

Clim Chang Responses

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Background: Human activities have led to a substantial increase in carbon dioxide (CO 2 ) emission, with further increases predicted. A RNA-Seq study on adult Saccostrea glomerata was carried out to examine the molecular response of this bivalve species to elevated pCO 2 . Results: A total of 1626 S. glomerata transcripts were found to be differentially expressed in oysters exposed to elevated pCO 2 when compared to control oysters. These transcripts cover a range of functions, from immunity (e.g. pattern recognition receptors, antimicrobial peptides), to respiration (e.g. antioxidants, mitochondrial respiratory chain proteins) and biomineralisation (e.g. carbonic anhydrase). Overall, elevated levels of CO 2 appear to have resulted in a priming of the immune system and in producing countermeasures to potential oxidative stress. CO 2 exposure also seems to have resulted in an increase in the expression of proteins involved in protein synthesis, whereas transcripts putatively coding for proteins with a role in cilia and flagella function were down-regulated in response to the stressor. In addition, while some of the transcripts related to biomineralisation were up-regulated (e.g. carbonic anhydrase 2, alkaline phosphatase), a small group was down-regulated (e.g. perlucin). Conclusions: This study highlighted the complex molecular response of the bivalve S. glomerata to expected near-future ocean acidification levels. While there are indications that the oyster attempted to adapt to the stressor, gauged by immune system priming and the increase in protein synthesis, some processes such cilia function appear to have been negatively affected by the elevated levels of CO 2 .

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“…Among these approaches, proteomics can theoretically present all the proteins encoded by the given genome in an organism [14]. The two-dimensional electrophoresis (2-DE)-based proteomics remains widely useful to characterize complex biologically functional protein networks [15]. Not only can proteomics describe a complete proteome at organelle, cell, or tissue levels, but it can also compare proteomes and then detect proteomic differences caused by either biotic or abiotic stressors [16].…”

Section: Introductionmentioning

confidence: 99%

Proteomic and metabolomic responses in hepatopancreas of Mytilus galloprovincialis challenged by Micrococcus luteus and Vibrio anguillarum

Wei

et al. 2013

Journal of Proteomics

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The outbreak of pathogens can induce diseases and lead to massive mortalities of aquaculture animals including fish, mollusk and shrimp. In this work, the responses induced by Micrococcus luteus and Vibrio anguillarum were investigated in hepatopancreas of mussel Mytilus galloprovincialis using proteomics and metabolomics. Metabolic biomarkers demonstrated that M. luteus and V. anguillarum injections could induce osmotic stress and disturbance in energy metabolism. And the uniquely and more markedly altered metabolic biomarkers (glutamine, succinate, aspartate, glucose, ATP, homarine and tyrosine) indicated that V. anguillarum could cause more severe disturbances in osmotic regulation and energy metabolism. The differentially altered proteins meant that M. luteus and V. anguillarum induced different effects in mussels. However, the common proteomic biomarkers, arginine kinase and small heat shock protein, demonstrated that these two bacteria induced similar effects including oxidative stress and disturbance in energy metabolism in M. galloprovincialis. In addition, some metabolic biomarkers, ATP and glutamine, were confirmed by related proteins including arginine kinase, ATP synthase, nucleoside diphosphate kinase and glutamine synthetase in bacteria-challenged mussels.This study demonstrated that proteomics and metabolomics could provide an insightful view into the effects of environmental pathogens to the marine mussel M. galloprovincialis. Biological significanceThe outbreak of pathogens can lead to diseases and massive mortalities of aquaculture animals including fish, mollusk and shrimp. The mussel M. galloprovincialis distributes widely along the Bohai coast and is popularly consumed as delicious seafood by local residents. This bivalve has become one of the important species in marine aquaculture industry in China. Therefore a study on pathogen-induced effects is necessary. In the present study, an integrated metabolomic and proteomic approach was used to elucidate the differential effects induced by the representative Gram-positive (M. luteus) and Gram-negative (V. anguillarum) bacteria in M. galloprovincialis.

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Proteomic identification of protein associated to mature spermatozoa in the Pacific oyster Crassostrea gigas

Cited by 20 publications

References 41 publications

The sperm proteome of the Pacific oysterCrassostrea gigasand immunolocalization of heat shock proteins

The sperm proteome of the Pacific oysterCrassostrea gigasand immunolocalization of heat shock proteins

Molecular analysis of the Sydney rock oyster (Saccostrea glomerata) CO2 stress response

Proteomic and metabolomic responses in hepatopancreas of Mytilus galloprovincialis challenged by Micrococcus luteus and Vibrio anguillarum

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