Proteomic Identification of eEF1A1 as a Molecular Target of Curcumol for Suppressing Metastasis of MDA-MB-231 Cells

Hu, Qi; Ning, Ling; Yu, Zanyang; Dou, Guojun; Li, Li

doi:10.1021/acs.jafc.7b00573

Cited by 19 publications

(5 citation statements)

References 38 publications

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“…Of note, the therapeutic value of EEF1A1 has been revealed in chronic lymphocytic leukemia, 38 hepatocarcinoma, 39 and breast cancer cells. 40 Our results exhibited the significant effect of silencing EEF1A1 on attenuating DLBCL growth both in vitro and vivo, thus providing experimental evidence for its further investigation…”

Section: Discussionsupporting

confidence: 61%

Expression and Clinical Value of Eukaryotic Translation Elongation Factor 1A1 (EEF1A1) in Diffuse Large B Cell Lymphoma

Gong

Shuang²

2021

IJGM

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Background:The eukaryotic translation elongation factor 1A1 (EEF1A1) participates in protein translation and has been reported to be involved in tumor progression such as hepatocellular carcinoma. Diffuse large B-cell lymphoma (DLBCL) is the most common lymphoid malignancy in adults. In the present study, we aimed to detect the expression of EEF1A1 in DLBCL and to analyze its relationship with prognosis. Methods: We reviewed medical records of DLBCL patients in our hospital and evaluated their expression level of EEF1A1 in tumor tissues using immunohistochemical (IHC) assay. The Chi-square method was used for correlation analysis. The Kaplan-Meier method with Log rank test was used for univariate analysis. Cox proportional hazards model was used for multivariate analysis. Cellular and mice models were introduced to validate its oncogenic role. Results: EEF1A1 expression in tumor cells was higher in certain DLBCL cases. Patients with higher EEF1A1 expression were more likely to have advanced tumor stage and poorer 5-year overall survival (OS) rates. EEF1A1 expression in tumor cells was an independent risk predictor for OS (P < 0.05). Cellular assays demonstrated that EEF1A1-shRNA significantly inhibited lymphoma cell proliferation. The study of xenografts further verified the effect of EEF1A1-shRNA on suppressing tumor growth in vivo. Conclusion: EEF1A1 positivity predicts short survival in DLBCL patients. For patients with higher EEF1A1 expression, more strategy such as anti-EEF1A1 antibody treatment should be developed.

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Section: Discussionsupporting

confidence: 61%

Expression and Clinical Value of Eukaryotic Translation Elongation Factor 1A1 (EEF1A1) in Diffuse Large B Cell Lymphoma

Gong

Shuang²

2021

IJGM

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“…Li identified that the promoter regulatory element of EEF1A1 was regulated by MALAT1 and over-expressed MALAT1 played an important role in the metastasis of BC ( 40 ). In addition, the decreased EEF1A1 expression via curcumol attributed to the suppression of BC metastasis ( 41 ). The function of S100B for inhibiting tumor migration was found in ER-negative BC and high expression of S100B was associated with better distant metastases-free survival in BC ( 42 ).…”

Section: Discussionmentioning

confidence: 99%

A six-gene signature related with tumor mutation burden for predicting lymph node metastasis in breast cancer

Wang¹,

Xu²,

Deng³

et al. 2021

Transl Cancer Res TCR

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Background: Breast cancer (BC) is one of the most common cancers worldwide and patients with lymph node metastasis always suffer from a worse prognosis. Tumor mutation burden (TMB) has been reported as a potential predictor for tumor behaviors. However, the correlation between TMB and lymph node metastasis of BC remains unclear. This study aimed to explore TMB-related biomarkers to predict the lymph node metastasis in BC patients.Methods: A total of 949 BC patients with RNA-seq data, mutation data and clinical data were obtained from The Cancer Genome Atlas (TCGA) database. We visualized mutation data by "maftools" package. We calculated TMB of each patient and investigated its association with lymph node metastasis. BC patients were divided into lymph node positive and negative groups and we respectively identified TMB-related and lymph node-related differentially expressed genes (DEGs) to figure out intersected genes. Functional enrichment analysis and protein-protein interaction (PPI) network were performed to observe relevant biological functions. We constructed a TMB-related signature for predicting lymph node metastasis through Logistic regression analysis. A validation database (GSE102484) from the Gene Expression Omnibus (GEO)database was downloaded to verify the accuracy.Results: Single nucleotide polymorphism (SNP) occupied the highest proportion in variant types while C>T appeared most frequently in single nucleotide variant (SNV). TMB was regarded as negatively correlated with lymph node metastasis in BC (P=0.003). We identified 125 common DEGs through venn diagram, which were enriched in vesicle localization, calcium signaling pathway and salmonella infection. A TMB-related signature based on six genes (BAHD1, PPM1A, PQLC3, SMPD3, EEF1A1 and S100B) had reliable efficacy for predicting lymph node metastasis in BC and was proven as an independent predictive factor. The accuracy of this signature was further validated by GSE102484 database.Conclusions: Our results indicated that TMB was associated with lymph node metastasis of BC. We built a TMB-related signature consisting of six genes which might function as a novel biomarker for predicting lymph node metastasis in BC.

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“…Curcumin (Cur) is a natural active substance extracted from the tubers of herbs such as Curcuma longa [ 1 ], Rhizoma zedoariae [ 2 ] and Radix curcumae [ 3 ], and it has broad application prospects in the fields of foods [ 4 , 5 ] and medicines [ 6 , 7 ]. It has a strong coloration ability and various physiological effects including anti-oxidation, anti-inflammation [ 8 ], anti-tumor [ 9 ], bacteriostasis, and promoting wound healing [ 10 ].…”

Section: Introductionmentioning

confidence: 99%

One-Pot Self-Assembly of Core-Shell Nanoparticles within Fibers by Coaxial Electrospinning for Intestine-Targeted Delivery of Curcumin

Hou

Zhang

et al. 2023

Foods

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Nanotechniques for curcumin (Cur) encapsulation provided a potential capability to avoid limitations and improve biological activities in food and pharmaceutics. Different from multi-step encapsulation systems, in this study, zein–curcumin (Z–Cur) core-shell nanoparticles could be self-assembled within Eudragit S100 (ES100) fibers through one-pot coaxial electrospinning with Cur at an encapsulation efficiency (EE) of 96% for ES100–zein–Cur (ES100–Z–Cur) and EE of 67% for self-assembled Z–Cur. The resulting structure realized the double protection of Cur by ES100 and zein, which provided both pH responsiveness and sustained release performances. The self-assembled Z–Cur nanoparticles released from fibermats were spherical (diameter 328 nm) and had a relatively uniform distribution (polydispersity index 0.62). The spherical structures of Z–Cur nanoparticles and Z–Cur nanoparticles loaded in ES100 fibermats could be observed by transmission electron microscopy (TEM). Fourier transform infrared spectra (FTIR) and X-ray diffractometer (XRD) revealed that hydrophobic interactions occurred between the encapsulated Cur and zein, while Cur was amorphous (rather than in crystalline form). Loading in the fibermat could significantly enhance the photothermal stability of Cur. This novel one-pot system much more easily and efficiently combined nanoparticles and fibers together, offering inherent advantages such as step economy, operational simplicity, and synthetic efficiency. These core-shell biopolymer fibermats which incorporate Cur can be applied in pharmaceutical products toward the goals of sustainable and controllable intestine-targeted drug delivery.

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Proteomic Identification of eEF1A1 as a Molecular Target of Curcumol for Suppressing Metastasis of MDA-MB-231 Cells

Cited by 19 publications

References 38 publications

Expression and Clinical Value of Eukaryotic Translation Elongation Factor 1A1 (EEF1A1) in Diffuse Large B Cell Lymphoma

Expression and Clinical Value of Eukaryotic Translation Elongation Factor 1A1 (EEF1A1) in Diffuse Large B Cell Lymphoma

A six-gene signature related with tumor mutation burden for predicting lymph node metastasis in breast cancer

One-Pot Self-Assembly of Core-Shell Nanoparticles within Fibers by Coaxial Electrospinning for Intestine-Targeted Delivery of Curcumin

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