Proteomic identification of differentially expressed proteins during the acquisition of somatic embryogenesis in oil palm (Elaeis guineensis Jacq.)

Silva, Rafael de Carvalho; Carmo, Lílian S.T.; Luis, Zanderluce Gomes; Silva, Luciano; Scherwinski‐Pereira, Jonny Everson; Mehta, Ângela

doi:10.1016/j.jprot.2014.03.013

Cited by 45 publications

(36 citation statements)

References 73 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…This protein functions as a molecular chaperone that may help callus and ARP formation and development. Our results are in agreement with other studies such as embryo development (Silva Rde et al 2014;zur Nieden et al 1995), seed maturation (Waters et al 1996), the small heat shock protein expression at the early stage of development then decline or even disappear. It also concludes that the heat shock proteins play an important regulatory role at the early stages of organ development.…”

Section: Functional Analysis Of the Identified Proteinssupporting

confidence: 93%

Comparative proteomic analysis of tetraploid black locust (Robinia pseudoacacia L.) cuttings in different phases of adventitious root development

Sheng

Zhao

Zhang

et al. 2014

Trees

View full text Add to dashboard Cite

Key message Comparative proteome analysis revealed the proteins that are differentially expressed during the processes of tetraploid black locust (Robinia pseudoacacia L.) adventitious root formation, which are involved in carbohydrate binding, energy and metabolism, and stress response. Abstract The tetraploid black locust (Robinia pseudoacacia L.) is an important fast-growing tree used in afforestation and is also a protein-rich feed for poultry. Currently, the primary approach for tetraploid black locust reproduction is through cutting propagation. To identify the specific proteins in the rooting process, including the formation of adventitious root primordium (ARP) and the elongation of adventitious root (EAR), a comparative proteomic analysis of the ARP and EAR of tetraploid black locust was performed to identify related proteins that may be involved in regulating ARP formation and the process of EAR. High-resolution two-dimensional electrophoresis (2-DE) followed by colloidal Coomassie staining and mass spectrometric (MS) analysis was used to identify differentially expressed proteins in ARP and EAR. A total of 84 protein spots showed significant expression changes by 2-DE and were successfully identified by MALDI TOF/ TOF MS/MS. Of these, 29 proteins were differentially expressed during the ARP phase, and the remaining 55 proteins did so in the EAR phase. The majority of identified proteins were classified into functional categories, including carbohydrate binding, energy and metabolism, protein degradation/folding and import, cellular cytoskeleton, hormonerelated and oxidation reactions, stress response, cell proliferation, and transcription regulation. Quantitative real-time PCR analysis was performed for selected proteins and showed that not all of the protein expression levels were consistent with the mRNA levels. This study provides the basis for further functional studies of differentially expressed proteins, which will contribute to the understanding of the biochemical processes in adventitious root primordium formation and the elongation of adventitious root. Abbreviations 2-DE Two-dimensional gel electrophoresis MALDI Matrix assisted laser desorption ionization TOF Time of flight TOF MS Time of flight mass spectrometry MS Mass spectrometry IntroductionTetraploid black locust (Robinia pseudoacacia L.) (TRP), which can be produced by doubling the chromosomes of a diploid black locust (2n 9 2) artificially, is a leguminous Communicated by R. Hampp. Electronic supplementary materialThe online version of this article (

show abstract

Section: Functional Analysis Of the Identified Proteinssupporting

confidence: 93%

Comparative proteomic analysis of tetraploid black locust (Robinia pseudoacacia L.) cuttings in different phases of adventitious root development

Sheng

Zhao

Zhang

et al. 2014

Trees

View full text Add to dashboard Cite

show abstract

“…Glycolysis, a key and ubiquitous metabolic pathway by which the plant cell converts carbohydrates to the energetic coin ATP, is a central pathway to generate energy and metabolic intermediaries that sustain the biosynthesis of intra- and extra-cellular molecules required by the cell. Proteins of the glycolysis pathway such as phosphofructokinase, fructose-1,6-biphosphate aldolase, glyceraldehyde-3-phosphate dehydrogenase, triosephosphate isomerase, phosphoglycerate mutase, 2,3-bisphosphoglycerate-independent phosphoglycerate mutase, phosphoglycerate kinase, enolase, and pyruvate decarboxylase were reported to be more abundant in the EC stage of African oil palm ( Silva et al, 2014 ), G. hirsutum ( Ge et al, 2017 ), maize ( Sun et al, 2013 ; Varhaníková et al, 2014 ; Ge et al, 2017 ), saffron ( Sharifi et al, 2012 ), Cyphomandra betacea ( Correia et al, 2012b ), Musa ( Kumaravel et al, 2017 ), Vitis vinifera ( Zhang et al, 2009 ), Larix principis-rupprechtii ( Beversdorf, 1987 ; Zhao et al, 2015a ), A. angustifolia ( dos Santos et al, 2016 ), P. nigra ( Klubicová et al, 2017 ), Theobroma cacao ( Niemenak et al, 2015 ), and sugarcane ( Heringer et al, 2017 ).…”

Section: Glycolysis-supplied Energy Leads Embryo Formationmentioning

confidence: 99%

“…In contrast to EC, early somatic embryo initiation displayed downregulation of some proteins related to detoxification, such as Mn superoxide dismutase in G. hirsutum ( Ge et al, 2014 ); CAT in C. delgadii ( Domzalska et al, 2017 ), and Q. suber ( Gomez-Garay et al, 2013 ); ascorbate peroxidase in Q. suber ( Gomez-Garay et al, 2013 ) and G. hirsutum ( Ge et al, 2014 ); and guaiacol peroxidase (GPX) in G. hirsutum ( Ge et al, 2014 ), although this enzyme is upregulated across PGR- or stress-dependent somatic embryo maturation ( Teyssier et al, 2011 ; Morel et al, 2014a ; Vale et al, 2014 ; Li et al, 2015 ). Notably, at the cotyledonar somatic embryo stage, GPX is even more abundant at the somatic embryo stage ( Rode et al, 2012 ) than its ZE counterpart ( Gomez-Garay et al, 2013 ; Mwangi et al, 2013 ; Morel et al, 2014b ; Silva et al, 2014 ; Niemenak et al, 2015 ).…”

Section: Ros and Counterpart Ros Scavengers During Somatic Embryogenementioning

confidence: 99%

Advanced Proteomic Approaches to Elucidate Somatic Embryogenesis

Aguilar-Hernández

Loyola‐Vargas

2018

Front. Plant Sci.

View full text Add to dashboard Cite

Somatic embryogenesis (SE) is a cell differentiation process by which a somatic cell changes its genetic program and develops into an embryonic cell. Investigating this process with various explant sources in vitro has allowed us to trace somatic embryo development from germination to plantlets and has led to the generation of new technologies, including genetic transformation, endangered species conservation, and synthetic seed production. A transcriptome data comparison from different stages of the developing somatic embryo has revealed a complex network controlling the somatic cell’s fate, suggesting that an interconnected network acts at the protein level. Here, we discuss the current progress on SE using proteomic-based data, focusing on changing patterns of proteins during the establishment of the somatic embryo. Despite the advanced proteomic approaches available so far, deciphering how the somatic embryo is induced is still in its infancy. The new proteomics techniques that lead to the quantification of proteins with different abundances during the induction of SE are opening this area of study for the first time. These quantitative differences can elucidate the different pathways involved in SE induction. We envisage that the application of these proteomic technologies can be pivotal to identifying proteins critical to the process of SE, demonstrating the cellular localization, posttranslational modifications, and turnover protein events required to switch from a somatic cell to a somatic embryo cell and providing new insights into the molecular mechanisms underlying SE. This work will help to develop biotechnological strategies for mass production of quality crop material.

show abstract

“…Secreted proteins (SCSBSD2032F10) ( Table 2 ) are of great importance for somatic embryogenesis because some of them have the ability to convert NE to E callus when added to the culture medium, such as like arabinogalactan proteins [ 93 ]. These proteins are likely to be used to enhance somatic embryogenesis protocols, to increase induction in non-responsive or low-embryogenic genotypes and to improve regeneration rates [ 94 , 95 ]. In these present study, the expression of this protein leads us to believe that this protein is also important in the maturation phase, during which we observed somatic embryo development in sugarcane.…”

Section: Discussionmentioning

confidence: 99%

Label-Free Quantitative Proteomics of Embryogenic and Non-Embryogenic Callus during Sugarcane Somatic Embryogenesis

et al. 2015

View full text Add to dashboard Cite

The development of somatic cells in to embryogenic cells occurs in several stages and ends in somatic embryo formation, though most of these biochemical and molecular changes have yet to be elucidated. Somatic embryogenesis coupled with genetic transformation could be a biotechnological tool to improve potential crop yields potential in sugarcane cultivars. The objective of this study was to observe somatic embryo development and to identify differentially expressed proteins in embryogenic (E) and non-embryogenic (NE) callus during maturation treatment. E and NE callus were cultured on maturation culture medium supplemented with different concentrations (0.0, 0.75, 1.5 and 2.0 g L-1) of activated charcoal (AC). Somatic embryo formation and differential protein expression were evaluated at days 0 and 21 using shotgun proteomic analyses. Treatment with 1.5 g L-1 AC resulted in higher somatic embryo maturation rates (158 somatic embryos in 14 days) in E callus but has no effect in NE callus. A total of 752 co-expressed proteins were identified through the SUCEST (The Sugarcane EST Project), including many housekeeping proteins. E callus showed 65 exclusive proteins on day 0, including dehydrogenase, desiccation-related protein, callose synthase 1 and nitric oxide synthase. After 21 days on maturation treatment, 14 exclusive proteins were identified in E callus, including catalase and secreted protein. NE callus showed 23 exclusive proteins on day 0 and 10 exclusive proteins after 21 days on maturation treatment, including many proteins related to protein degradation. The induction of maturation leads to somatic embryo development, which likely depends on the expression of specific proteins throughout the process, as seen in E callus under maturation treatment. On the other hand, some exclusive proteins can also specifically prevent of somatic embryos development, as seen in the NE callus.

show abstract

Proteomic identification of differentially expressed proteins during the acquisition of somatic embryogenesis in oil palm (Elaeis guineensis Jacq.)

Cited by 45 publications

References 73 publications

Comparative proteomic analysis of tetraploid black locust (Robinia pseudoacacia L.) cuttings in different phases of adventitious root development

Comparative proteomic analysis of tetraploid black locust (Robinia pseudoacacia L.) cuttings in different phases of adventitious root development

Advanced Proteomic Approaches to Elucidate Somatic Embryogenesis

Label-Free Quantitative Proteomics of Embryogenic and Non-Embryogenic Callus during Sugarcane Somatic Embryogenesis

Contact Info

Product

Resources

About