Proteomic identification of camel seminal plasma: Purification of β-nerve growth factor

Identification of beta-nerve growth factor in dromedary camel seminal plasma and its role in induction of ovulation in females

“…The proportion of this protein in CSP was considerably higher than that found by Druart et al (2013) in dromedary camel seminal plasma (24%) and comparable to that in alpaca (47%).Previous studies on small camelids showed that β-NGF is a 26 ~27 kDa homodimer that is reduced to 2 dimers of approximately 14 kDa under denaturing conditions (Kershaw-Young et al, 2012;Ratto et al, 2012).Although, a proteomic study conducted by Kumar et al (2013) revealed a 22 kDa molecular mass of β-NGF purified from dromedary camel seminal plasma.…”

“…Two protein peaks were isolated from the seminal plasma using gel exclusion chromatography, similar to that previously reported for llama seminal plasma using a combination of hydroxyapatite and gel-filtration chromatography . However, six protein fractions were obtained after separation of Bactrian camel seminal plasma (Li and Zhao, 2004) and three protein peaks were eluted after purification of dromedary camel seminal plasma using a sequential separation procedure (Kumar et al, 2013). The variability of protein fractions could be explained by the differences in the purification procedures and the protein concentration in seminal plasma of camelid species.…”

“…More recent, a proteomic study revealed that OIF from llama has a molecular mass of 13.221 kDa, and a database search showed that the 12-23 amino-acid sequence of OIF had a high homology with the sequence of the β-Nerve Growth Factor (β-NGF) of human, porcine, bovine and murine species . The isolation and identification of β-NGF from dromedary camel seminal plasma was conducted by Kumar et al (2013), who showed that this factor has a molecular mass of 22 kDa with a MASCOT score of 118 and 2 matched peptides. However, the ovulation inducing capacity of dromedary camel β-NGF has not been demonstrated.…”

Early Embryonic Development and Maternal Recognition of Pregnancy in Camelids: A Review

“…The preovulatory luteinizing hormone (LH) surge occurs after mating in response to an ovulation inducing factor (OIF) in seminal plasma. Recently, this OIF has been identified as β nerve growth factor (βNGF) (Adams and Ratto, 2013;Kershaw-Young et al, 2012;Kumar et al, 2013). It is important to note that βNGF may have a luteotrophic effect on the corpus luteum (CL) (Silva et al, 2014).…”

Selection of downstream steps by analysis of protein surface property: A case study for recombinant human lactoferrin purification from milk of transgenic cow

“…Experience prediction, on the other hand, is useful. For example, cationic exchange chromatography is proposed as the top priority in purification of basic or alkaline proteins which demonstrate high isoelectric points [4,5]. Size exclusion chromatography is often employed for virus-like particle purification because of its much greater size than other protein impurities [6,7].…”