Proteomic Identification of a Monoclonal Antibody Recognizing Caveolin-1 in Hepatocellular Carcinoma with Metastatic Potential

Hu, Man; Lam, Chi-Tat; Liu, Kang‐Da; Xu, Zhi; Fatima, Sarwat; Su, Yvonne C. F.; Tsang, Felice Ho‐Ching; Chen, Jie; Pang, Jin-Zhong; Qin, Lun‐Xiu; Luk, John M.

doi:10.2174/092986609788167743

Cited by 5 publications

(3 citation statements)

References 25 publications

(28 reference statements)

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“…The interactive partner of OPN, CD44, and its isomer v6, were confirmed to be highly expressed in HCCs and was connected to cell motility likely through cell skeleton complex of CD44-ERM [11,12]. CAV1, a metastatic promoter, was confirmed as a therapeutic target in HCC with metastatic potential [13,14]. ANXA2 and S100A4 were also found to be highly expressed in HCC samples [5].…”

Section: Discrete Significant Genes Were Screened From Transcriptome mentioning

confidence: 94%

Transcriptome and proteome of human hepatocellular carcinoma reveal shared metastatic pathways with significant genes

et al. 2015

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Previously isolated pathways screened from individual genes were investigated at either the transcriptional or translational level; however, the consistency between the pathways screened at the gene expression levels was obscure in metastatic human hepatocellular carcinoma (HCC). To elucidate this question, we performed a transcriptomic (16,353 genes) and proteomic (7861 proteins) analysis simultaneously on six metastatic HCC cell lines against two nonmetastatic HCC cell lines, with all HBV traceable and close genetic-backgrounds for a comparative study. The quantitative and integrated results showed that significant genes were screened differentially with 351 transcripts from the transcriptome and 304 proteins from the proteome, with limited overlapping genes (7%). However, we discovered that these discrete 351 transcripts and 304 proteins screened share extrusive significant-pathways/networks with a 77% overlap, including active TGF-β, RAS, NFκB, and Wnt, and inactive HNF4A, which are responsible for HCC metastasis. We conclude that the discrete, but significant genes predicted by either ome play intrinsically important roles in the linkage of responsible pathways shared by both omes in HCC metastasis.

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Section: Discrete Significant Genes Were Screened From Transcriptome mentioning

confidence: 94%

Transcriptome and proteome of human hepatocellular carcinoma reveal shared metastatic pathways with significant genes

et al. 2015

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“…Cav-1 expression was upregulated in inflammatory breast cancer cells and tissues [ 53 ] and Cav-1 overexpression significantly contributed to cancer metastasis. With using proteomic identification, Cav-1 was found to be elevated in metastatic hepatocellular cancer cell lines [ 54 ]. A microarray analysis also supported Cav-1 as a metastasis-related gene by comparing gene expression profiles between weakly and highly invasive breast cancer cells [ 55 ].…”

Section: Cav-1 and Cancer Developmentmentioning

confidence: 99%

“…Cav-1 loss not only inactivate tumor suppressors BRCA1, P53 and PTEN but also promoted cell cycle progression and activated pro-survival signaling such as PI3K/Akt and MAPK [ 36 , 38 , 70 ]. However, with cancer advanced progression, Cav-1 re-expression was observed in various cancers including breast, lung, prostate, liver, ovarian, pancreas, melanoma, thyroid, colorectal, gastric, renal and pleomorphic malignancies [ 51 , 54 , 57 , 71 - 79 ]. Cav-1 overexpression was found in metastatic lesions of breast cancer and was closely correlated with tumor stage and clinical prognosis [ 80 ].…”

Section: Cav-1 and Cancer Developmentmentioning

confidence: 99%

Caveolin-1, a stress-related oncotarget, in drug resistance

Wang

Liu

et al. 2015

Oncotarget

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Caveolin-1 (Cav-1) is both a tumor suppressor and an oncoprotein. Cav-1 overexpression was frequently confirmed in advanced cancer stages and positively associated with ABC transporters, cancer stem cell populations, aerobic glycolysis activity and autophagy. Cav-1 was tied to various stresses including radiotherapy, fluid shear and oxidative stresses and ultraviolet exposure, and interacted with stress signals such as AMP-activated protein kinase. Finally, a Cav-1 fluctuation model during cancer development is provided and Cav-1 is suggested to be a stress signal and cytoprotective. Loss of Cav-1 may increase susceptibility to oncogenic events. However, research to explore the underlying molecular network between Cav-1 and stress signals is warranted.

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Discovery of Lamin B1 and Vimentin as Circulating Biomarkers for Early Hepatocellular Carcinoma

Wong

Luk

2012

Liver Proteomics

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The recent advancements in proteomic technologies have reconstituted our research strategies over different type of liver diseases including hepatocellular carcinoma (HCC). Combined analyses on HCC proteome and clinicopathological data of patients have allowed identification of many promising biomarkers that can be further developed into noninvasive diagnostic assays for cancer surveillance. Capitalizing our established proteomic platform primarily based on two-dimensional polyacrylamide gel electrophoresis (2DE) and MALDI-TOF/TOF mass spectrometry, our groups have identified lamin B1 (LMNB1) and vimentin (VIM) as promising biomarkers for detection of early HCC. Protein levels of both biomarkers were significantly elevated in cancerous tissues when compared to the controls in disease-free and cirrhotic liver subjects. Further investigation of the circulating LMNB1 mRNA level in patients' blood samples by standard PCR showed 76% sensitivity and 82% specificity for detection of early HCC. In parallel, an ELISA assay for measuring circulating vimentin level in patients' serum samples could detect small HCC at 40.91% sensitivity and 87.5% specificity. The candidate biomarkers were evaluated with the diagnostic performance of α-fetoprotein (AFP) for HCC. In this article, we address the current protocols for HCC biomarker discovery, ranging from clinical sample preparation, 2DE proteomic profiling and informatics analysis, and assay development and clinical validation study. Focus is emphasized on the methods for sample preservation and low-abundance protein enrichment.

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Proteomic Identification of a Monoclonal Antibody Recognizing Caveolin-1 in Hepatocellular Carcinoma with Metastatic Potential

Cited by 5 publications

References 25 publications

Transcriptome and proteome of human hepatocellular carcinoma reveal shared metastatic pathways with significant genes

Transcriptome and proteome of human hepatocellular carcinoma reveal shared metastatic pathways with significant genes

Caveolin-1, a stress-related oncotarget, in drug resistance

Discovery of Lamin B1 and Vimentin as Circulating Biomarkers for Early Hepatocellular Carcinoma

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