Proteomic evaluation of human umbilical cord tissue exposed to polybrominated diphenyl ethers in an e-waste recycling area

Li, Minghui; Huo, Xia; Pan, Yukui; Cai, Haoxing; Dai, Yifeng; Xu, Xijin

doi:10.1016/j.envint.2017.09.016

Cited by 41 publications

(18 citation statements)

References 71 publications

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“…At least one unique peptide was necessary for identification of a protein. The labeled peptides by isobaric tags for quantification analysis were performed using IQuant software as previously described [55]. To assess the confidence of the peptides, peptide-spectrum matches (PSMs) were pre-filtered at a PSM-level false discovery rate (FDR) of 1%.…”

Section: Methodsmentioning

confidence: 99%

Comparative proteomics analysis of peanut roots reveals differential mechanisms of cadmium detoxification and translocation between two cultivars differing in cadmium accumulation

Jiang

et al. 2019

BMC Plant Biol

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Background Peanut is one of the most important oil and protein crops, and it exhibits wide cultivar variations in shoot Cd accumulation ability. However, the mechanism of Cd accumulation in peanut shoots has not been well understood. In this study, the root proteomics of two cultivars differing in seed Cd accumulation, Fenghua 1 (F, low Cd cultivar) and Silihong (S, high Cd cultivar), were investigated under 0 (CK) and 2 μM Cd conditions. Results A total of 4676 proteins were identified by proteomics screening. Of them, 375, 1762, 1276 and 771 proteins were identified to be differentially expressed proteins (DEPs) for comparison of F Cd /F CK , S Cd /S CK , F CK /S CK and F Cd /S Cd , respectively. Silihong is more sensitive to Cd exposure than Fenghua 1 in terms of root proteomics. A total of 30 and 86 DEPs were identified to be related with heavy metal transport and cell wall modification, respectively. The up-regulation of ABCB25, ABCC14, ABCC2, PDR1 and V-ATPases by Cd exposure in Silihong might enhance vacuolar sequestration of Cd and its efflux from symplast to apoplast. The higher Cd accumulation in the root CWs of Silihong might be resulted from its higher capability of CW modification, in which many proteins such as IRX10L, BGLU12-like, BGLU42, EXLB1, XTH30, XTH6, XYL7, PAL3, COMT, CAD1, and CCR1 were involved. Conclusions The vacuolar sequestration and efflux of Cd as well as its adsorption in CW might be the principal mechanism of cadmium detoxification in Silihong. The higher capacity of Cd accumulation and translocation of Silihong is an inherent characteristics in which ACA8 and ZIP1 might be involved. Electronic supplementary material The online version of this article (10.1186/s12870-019-1739-5) contains supplementary material, which is available to authorized users.

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Section: Methodsmentioning

confidence: 99%

Comparative proteomics analysis of peanut roots reveals differential mechanisms of cadmium detoxification and translocation between two cultivars differing in cadmium accumulation

Jiang

et al. 2019

BMC Plant Biol

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“…Upon completion of the co-culture assay, the IPEC-J2 cells were washed three times with sterile PBS buffer to remove the L. gasseri LA39. The IPEC-J2 cells were frozen with liquid nitrogen immediately and then lysed in the “lysis buffer (8 M Urea, 40 mM Tris–HCl with 1 mM PMSF, 2 mM EDTA, and 10 mM DTT, pH 8.5)” (Li et al, 2018). “After centrifugation at 25,000 g at 4°C for 20 min, the supernatant obtained was reduced with 10 mM DTT at 56°C for 1 h, and then alkylated with 55 mM iodoacetamide (IAM) in the dark at room temperature for 45 min” (Huang et al, 2018).…”

Section: Methodsmentioning

confidence: 99%

“…Following trypsin digestion, “the peptides were desalted, vacuum-dried, and dissolved in 30 μl of 0.5 M TEAB and labeled by the iTRAQ Reagent 8-plex Kit. The peptides labeled with different reagents were combined, and then desalted and vacuum-dried” (Li et al, 2018).…”

Section: Methodsmentioning

confidence: 99%

Lactobacillus gasseri LA39 Activates the Oxidative Phosphorylation Pathway in Porcine Intestinal Epithelial Cells

Zheng

et al. 2018

Front. Microbiol.

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Intestinal microbial interactions with the host epithelium have important roles in host health. Our previous data have suggested that Lactobacillus gasseri LA39 is the predominant intestinal Lactobacillus in weaned piglets. However, the regulatory role of L. gasseri LA39 in the intestinal epithelial protein expression in piglets remains unclear. In the present study, we conducted comparative proteomics approach to investigate the intestinal epithelial protein profile alteration caused by L. gasseri LA39 in piglets. The expressions of 15 proteins significantly increased, whereas the expressions of 13 proteins significantly decreased in the IPEC-J2 cells upon L. gasseri LA39 treatment. Bioinformatics analyses, including COG function annotation, GO annotation, and KEGG pathway analysis for the differentially expressed proteins revealed that the oxidative phosphorylation (OXPHOS) pathway in IPEC-J2 cells was significantly activated by L. gasseri LA39 treatment. Further data indicated that two differentially expressed proteins UQCRC2 and TCIRG1, associated with the OXPHOS pathway, and cellular ATP levels in IPEC-J2 cells were significantly up-regulated by L. gasseri LA39 treatment. Importantly, the in vivo data indicated that oral gavage of L. gasseri LA39 significantly increased the expression of UQCRC2 and TCIRG1 and the cellular ATP levels in the intestinal epithelial cells of weaned piglets. Our results, both in vitro and in vivo, reveal that L. gasseri LA39 activates the OXPHOS pathway and increases the energy production in porcine intestinal epithelial cells. These findings suggest that L. gasseri LA39 may be a potential probiotics candidate for intestinal energy production promotion and confers health-promoting functions in mammals.

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“…For instance, BFRs are endocrine disruptors (Eguchi et al, 2015;Guo et al, 2018Guo et al, , 2019bZheng et al, 2017aZheng et al, , 2017b, and could exert adverse effects on human semen quality (Yu et al, 2018); while OPFRs have been associated with greater DNA damage (Lu et al, 2017). Moreover, health effects of HFR and OPFR exposure on fetuses and infants are of particular concern due to their substantially weaker resistance and immunity (Bai et al, 2019;Li et al, 2018;Xu et al, 2015;Zheng et al, 2017b).…”

Section: Introductionmentioning

confidence: 99%

Human exposure to halogenated and organophosphate flame retardants through informal e-waste handling activities - A critical review

Stubbings

Cline‐Cole

et al. 2021

Environmental Pollution

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Proteomic evaluation of human umbilical cord tissue exposed to polybrominated diphenyl ethers in an e-waste recycling area

Cited by 41 publications

References 71 publications

Comparative proteomics analysis of peanut roots reveals differential mechanisms of cadmium detoxification and translocation between two cultivars differing in cadmium accumulation

Comparative proteomics analysis of peanut roots reveals differential mechanisms of cadmium detoxification and translocation between two cultivars differing in cadmium accumulation

Lactobacillus gasseri LA39 Activates the Oxidative Phosphorylation Pathway in Porcine Intestinal Epithelial Cells

Human exposure to halogenated and organophosphate flame retardants through informal e-waste handling activities - A critical review

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