Proteomic evaluation and location of UVB-induced photo-oxidation in wool

Dyer, Jolon M.; Plowman, Jeffrey E.; Krsinic, Gail L.; Deb‐Choudhury, Santanu; Koehn, Henning; Millington, Keith R.; Clerens, Stefan

doi:10.1016/j.jphotobiol.2009.11.008

Cited by 47 publications

(40 citation statements)

References 25 publications

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“…Individual marker peptides are then monitored and tracked. Another emerging approach to profile protein modifications is to utilise a scoring system, where different modifications are assigned a score and the range of modifications observed at a certain MS/MS identification threshold contribute to the overall score (Dyer et al, 2010). The data can be broken down to evaluate selected modification types, and scores between samples allow effective comparison and contrast.…”

Section: Profiling and Trackingmentioning

confidence: 99%

“…Our previous work has allowed us to develop an advanced understanding of the photochemistry underpinning oxidative modification of proteins subjected to UVB irradiation (Dyer et al, 2006a;Dyer et al, 2006b;Dyer et al, 2009;Dyer et al, 2010;Grosvenor et al, 2010a). Therefore, UVB irradiation was chosen as the method to reliably induce oxidative modifications in dairy proteins.…”

Section: Proof Of Concept -Lactoferrin and β-Lactoglobulinmentioning

confidence: 99%

“…A collection of Visual Basic scripts was developed to remove redundancy, count, weight, score and sum the different damage modifications. Essentially, each targeted modification is given a factor reflecting its severity, based on its rank in the modification pathway (Dyer et al, 2010). The number of occurrences of each modification (multiplied by the modification factor) is then weighted against the number of observed susceptible residues.…”

Section: Proof Of Concept -Lactoferrin and β-Lactoglobulinmentioning

confidence: 99%

“…Understanding of protein damage at the amino acid residue level and correlation with processing parameters will ultimately allow process improvement and mitigation/repair of protein damage. We have previously used a damage scoring system for the characterisation of UVB-induced photo-oxidation in wool (Dyer 2010). This system allowed us to assign an absolute numerical value to the degree of damage in the samples under investigation.…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Food Proteomics: Mapping Modifications

Clerens¹,

Plowman²,

Dyer³

2012

Proteomic Applications in Biology

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Section: Profiling and Trackingmentioning

confidence: 99%

Section: Proof Of Concept -Lactoferrin and β-Lactoglobulinmentioning

confidence: 99%

Section: Proof Of Concept -Lactoferrin and β-Lactoglobulinmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Food Proteomics: Mapping Modifications

Clerens¹,

Plowman²,

Dyer³

2012

Proteomic Applications in Biology

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“…Recent mass spectrometry-based characterisations of damaged wool and skin proteins [5][6][7][8][9] allowed the identification of damaged residues within specific proteins, and new proteomic methods have also been developed to quantify and classify oxidative damage within peptides and proteins [8,10,11].…”

Section: Introductionmentioning

confidence: 99%

Determination and Validation of Markers for Heat-Induced Damage in Wool Proteins

Grosvenor¹,

Morton²,

Dyer³

2012

AJAC

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Protein-based animal fibres of commercial importance are frequently exposed to elevated temperatures during processing treatments. Hydrothermal processes cause protein deterioration, impacting negatively on the value or condition of these materials. This study was designed to investigate hydrothermal damage in wool proteins at the molecular level. The effect of hydrothermal damage on Type I and II intermediate filament proteins (keratins) extracted from wool was characterised using advanced quantitative techniques based on isobaric iTRAQ labelling and mass spectrometry. Many native peptides were observed to be degraded and modified. Amongst these, twenty keratin peptides were observed to consistently degrade during hydrothermal exposure. These peptides acted as molecular markers of damage-specific indicators of the extent of heat-induced protein damage. This technology will be of value in assessing the severity of damage imparted after high temperature exposure of protein-based animal fibres such as wool and cashmere during processes such as dyeing and carbonising, or even after high temperature human hair treatments. The identification of molecular damage markers identified within wool and other materials provides a new route to sensitive and specific evaluation of the effects of protein deterioration. It is anticipated that the utilisation of such markers will facilitate the development of targeted approaches to minimising processing damage to high-value fibres and protein-based biomaterials.

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