Proteomic characterization of seminal plasma from alternative reproductive tactics of Chinook salmon ( Oncorhynchus tswatchysha )

Gombar, Robert; Pitcher, Trevor E.; Lewis, Jason A.; Auld, J.; Vacratsis, Panayiotis O.

doi:10.1016/j.jprot.2017.01.019

Cited by 23 publications

(18 citation statements)

References 82 publications

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“…Much less is known in fish, but ion content of the medium appears to be crucial for sperm activation (Alavi and Cosson, 2006), whereas proteins, monosaccharides and triglycerides appear to affect sperm viability and speed (Lahnsteiner et al, 2004;Lahnsteiner, 2007). Support for the role of seminal fluid protein abundance in the plasticity of sperm performances in response to sperm competition risk comes not only from the house mouse and the Australian field cricket (Ramm et al, 2015;Simmons and Lovegrove, 2017) but also from the Chinook salmon (Gombar et al, 2017) and the grass goby (F.P., unpublished data), where the seminal fluid protein profile differs in relation to male tactic. Accordingly, in the black goby, the seminal fluid of territorial males might be richer in protein content, to enhance the fertilization ability of their sperm, whereas that of sneaker males might be poorer in protein but endowed with an ionic composition adequate to properly regulate sperm activation.…”

Section: Discussionmentioning

confidence: 99%

Seminal fluid enhances competitiveness of territorial males’ sperm in a fish with alternative male reproductive tactics

Poli

Locatello

Rasotto

2018

Journal of Experimental Biology

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The most common adaptation to sperm competition in males is represented by an increase in the sperm number and/or quality released at mating, to raise their probability of egg fertilization. However, rapidly mounting evidence highlights that seminal fluid may directly influence the competitive fertilization success of a male by affecting either own and/or rival sperm performance. In the black goby, , an external fertilizer with guard-sneaker mating tactics and high sperm competition level, sneaker ejaculates contain less seminal fluid and more sperm, that are also of better quality, than those of territorial males. However, territorial males gain a higher paternity success inside natural nests. Here, we ask whether the seminal fluid can contribute to the reproductive success of territorial males by enhancing their sperm performance and/or by decreasing that of sneaker males. Using sperm and seminal fluid manipulation and fertilization tests, we found that own seminal fluid influences the velocity and fertilization ability of sperm only in territorial males, making them as fast as those of sneakers and with a similar fertilization rate. Moreover, both sneaker and territorial sperm remain unaffected by the seminal fluid of rival males. Thus, black goby males respond to the different level of sperm competition faced by differential allocation of sperm and non-sperm components of the ejaculate, with sneakers primarily investing in sperm of intrinsic high quality and territorial males relying on the effect of seminal fluid to increase the lower intrinsic quality of their sperm.

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Section: Discussionmentioning

confidence: 99%

Seminal fluid enhances competitiveness of territorial males’ sperm in a fish with alternative male reproductive tactics

Poli

Locatello

Rasotto

2018

Journal of Experimental Biology

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“…Campbell et al 1992). It is important to pursue questions related to mate choice in salmonids and polyploids in general, using traditional methodologies, such as behavioural studies together with novel methodologies, including the incorporation of genetic techniques, transcriptomics and proteomics (Makiguchi et al 2016b;Gombar et al 2017) within the same system. The complementary use of methodologies within a single system may allow researchers to answer questions related to mating behaviour and reproductive success that would not be possible with traditional behavioural studies (Archie and Chiyo 2011).…”

Section: Mechanisms and Trendsmentioning

confidence: 99%

Advancing mate choice studies in salmonids

Auld

Noakes

Banks

2019

Rev Fish Biol Fisheries

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Mate choice in most organisms is not random, but determined by a suite of interacting traits and environmental factors. While the selective pressures underlying differences in mate choice between species, populations, individuals and even within individuals has been gaining interest, there still remains unexplained variation in mate preferences especially in non-model systems. Despite being of social, environmental and economic importance there is comparatively little known about how salmonids and other tetraploids make mate choice decisions in the wild and the resultant reproductive success (i.e. the number of offspring which survive to sexual maturity). Resolving questions related to salmonid mate choice is of particular importance given that humans have been supplementing salmon populations through aquaculture for decades. Despite these efforts, hatchery produced fish have lower reproductive success relative to their wild counterparts and salmon populations are declining. Most studies on mate choice and reproductive success in salmonids focus on body size and major histocompatibility complex based choice. However, mate choice can also be affected by other factors including other genetic factors, predation risk and social environment. Here, we (a) synthesize what is presently known about mate choice and reproductive success in salmonids, (b) identify gaps in knowledge and areas where there is a lack of consensus in results, and (c) suggest interdisciplinary ways of advancing our understanding of mate choice in salmonids and other polyploids.

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“…How these factors affect otolith morphology has received minimal research attention to date. This is rather surprising because many commercially‐important fishes, such as salmonids, express distinct male alternative reproductive tactics (Berejikian et al, ; Gombar et al, ; Jones, ) and anthropogenic pollution is of increasing concern for aquatic ecosystems (Bernhardt et al, ; Strayer & Dudgeon, ).…”

Section: Introductionmentioning

confidence: 99%

Growth and otolith morphology vary with alternative reproductive tactics and contaminant exposure in the round goby Neogobius melanostomus

Bose

McCallum

Raymond

et al. 2018

Journal of Fish Biology

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Round goby Neogobius melanostomus sagittal (saccular) otolith morphology was compared between males of the two alternative reproductive tactics (termed guarder and sneaker males) and between males captured from sites of high or low contamination. Otolith size increased with fish size and also displayed an ontogenetic shift in shape, becoming relatively taller as otoliths grew in size. Despite a considerable overlap in age between males adopting the two reproductive tactics, size-at-age measurements revealed that guarder males are significantly larger than sneakers at any given age and that they invest more into somatic growth than sneaker males. Controlling for body size, sneaker males possessed heavier sagittal otoliths than guarder males. Subtle otolith shape differences were also found between the two male tactics and between sites of high and low contaminant exposure. Sneaker males had relatively shorter otoliths with more pronounced notching than guarder males. Fish captured at sites of high contamination had otoliths showing slower growth rates in relation to body size and their shapes had more pronounced caudal points and ventral protrusions when compared with fish captured at sites of low contamination. The results are discussed in relation to life-history tradeoffs between the male tactics in terms of reproductive and somatic investment as well as the putative metabolic costs of exposure to contaminants. Overall, this study reveals that male alternative reproductive tactics and environmental contaminants can have small, yet measurable, effects on otolith morphology and these factors should be accounted for in future research when possible.

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Proteomic characterization of seminal plasma from alternative reproductive tactics of Chinook salmon ( Oncorhynchus tswatchysha )

Cited by 23 publications

References 82 publications

Seminal fluid enhances competitiveness of territorial males’ sperm in a fish with alternative male reproductive tactics

Seminal fluid enhances competitiveness of territorial males’ sperm in a fish with alternative male reproductive tactics

Advancing mate choice studies in salmonids

Growth and otolith morphology vary with alternative reproductive tactics and contaminant exposure in the round goby Neogobius melanostomus

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