Proteomic Characterization of Proliferation Inhibition of Well-Differentiated Laryngeal Squamous Cell Carcinoma Cells Under Below-Background Radiation in a Deep Underground Environment

Abstract

Background: There has been a considerable concern about cancer induction in response to radiation exposure. However, only a limited number of studies have focused on the biological effects of below-background radiation (BBR) in deep underground environments. To improve our understanding of the effects of BBR on cancer, we studied its biological impact on well-differentiated laryngeal squamous cell carcinoma cells (FD-LSC-1) in a deep underground laboratory (DUGL).Methods: The growth curve, morphological, and quantitative proteomic experiments were performed on FD-LSC-1 cells cultured in the DUGL and above-ground laboratory (AGL).Results: The proliferation of FD-LSC-1 cells from the DUGL group was delayed compared to that of cells from the AGL group. Transmission electron microscopy scans of the cells from the DUGL group indicated the presence of hypertrophic endoplasmic reticulum (ER) and a higher number of ER. At a cutoff of absolute fold change ≥ 1.2 and p < 0.05, 807 differentially abundant proteins (DAPs; 536 upregulated proteins and 271 downregulated proteins in the cells cultured in the DUGL) were detected. KEGG pathway analysis of these DAPs revealed that seven pathways were enriched. These included ribosome (p < 0.0001), spliceosome (p = 0.0001), oxidative phosphorylation (p = 0.0001), protein export (p = 0.0001), thermogenesis (p = 0.0003), protein processing in the endoplasmic reticulum (p = 0.0108), and non-alcoholic fatty liver disease (p = 0.0421). Conclusion:The BBR environment inhibited the proliferation of FD-LSC-1 cells. Additionally, it induced changes in protein expression associated with the ribosome, gene spliceosome, RNA transport, and energy metabolism among others. The changes Liu et al.FD-LSC-1 Under Below Background Radiation in protein expression might form the molecular basis for proliferation inhibition and enhanced survivability of cells adapting to BBR exposure in a deep underground environment. RPL26,

Development of an Oxidative Phosphorylation-Related and Immune Microenvironment Prognostic Signature in Uterine Corpus Endometrial Carcinoma

“…Except for ATP5IF1, other candidate genes included in the prognostic signature have been proven to play a vital role in cancer development. COX6B1, as one of the core proteins involved in the molecular basis of the below-background radiation stress response, might inhibit the proliferation of laryngeal squamous cell carcinoma cells ( Liu et al, 2020b ). FOXP3 can activate the Wnt/beta-catenin signaling pathway, thus, inducing epithelial–mesenchymal transition and promoting the progression of non-small cell lung cancer ( Yang et al, 2017 ).…”

RPN2 Predicts Poor Prognosis and Promotes Bladder Cancer Growth and Metastasis via the PI3K-Akt Pathway

“…As shown in Figure 1E, RPN2 can regulate multiple genes such as RPN1, STT3A/B, and MAGT1, which are usually associated with tumor progression in various cancers. [17][18][19][20]…”

First transcriptome profiling of D. melanogaster after development in a deep underground low radiation background laboratory

“…At present the significant part of the effects registered in decreased background radiation conditions can be classified as suppressive, that reduce growth and development parameters, both for unicellular and multicellular organisms. Growth rate inhibition, increased sensitivity to radiation, delayed recovery after return to natural background conditions, alteration in expression of several genes concerned with ribosomal proteins, membrane transport, respiration, reparation and antioxidant regulation for increased ROS removal were observed in experiments on mammalian cell cultures (TK6, V79, M10, L5178Y, FD-LSC-1) and unicellular organisms ( Paramecium tetraurelia , Synechococcus lividus , Shewanella oneidensis , Deinococcus radiodiodurans , Saccharomyces cerevisiae ) [ 11 , 12 , 14 , 17 , 18 , 24 , 25 ]. The first multicellular organism used for determination of the effects of low-radiation background was the fruit fly in projects FLYINGLOW and RENOIR [ 20 , 26 ].…”