Proteomic characterization of oxidative dysfunction in human umbilical vein endothelial cells (HUVEC) induced by exposure to oxidized LDL

Kinumi, Tomoya; Ogawa, Yoko; Kimata, Junko; Saito, Yoshiro; Yoshida, Yasukazu; Niki, Etsuo

doi:10.1080/10715760500306695

Cited by 35 publications

(16 citation statements)

References 35 publications

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“…Studies also link NPM to the onset of atherosclerosis. Human vascular endothelial cells treated with oxidized low density lipoprotein show dephosphorylated levels of NPM correlating with decreased proliferation and increased cellular dysfunction [66], consistent with studies demonstrating enhanced endothelial cell proliferation correlating to increased NPM phosphorylation upon treatment with cardioprotective drugs [67]. …”

Section: Nucleolar Proteins Associated With Cardiac Pathophysiologysupporting

confidence: 62%

Stressing on the nucleolus in cardiovascular disease

Hariharan

Sussman

2014

Biochimica et Biophysica Acta (BBA) - Molecular Basis of Diseas

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The nucleolus is a multifunctional organelle with multiple roles involving cell proliferation, growth, survival ribosome biogenesis and stress response signaling. Alteration of nucleolar morphology and architecture signifies an early response to increased cellular stress. This review briefly summarizes nucleolar response to cardiac stress signals and details the role played by nucleolar proteins in cardiovascular pathophysiology.

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Section: Nucleolar Proteins Associated With Cardiac Pathophysiologysupporting

confidence: 62%

Stressing on the nucleolus in cardiovascular disease

Hariharan

Sussman

2014

Biochimica et Biophysica Acta (BBA) - Molecular Basis of Diseas

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“…On the other hand, phosphorylation on one or more of its four serine residues reduces its MT‐destabilizing activity. Alteration in stathmin expression has been reported in human malignancies (Belletti et al, 2008) and in HUVEC cells under oxidative stress condition (Kinumi et al, 2005). Our data reported show, for the first time, that also weightlessness heavily affects the expression levels of this protein (Figures 2 and 3A).…”

Section: Resultsmentioning

confidence: 99%

Protein pattern ofXenopus laevisembryos grown in simulated microgravity

et al. 2011

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Numerous studies indicate that microgravity affects cell growth and differentiation in many living organisms, and various processes are modified when cells are placed under conditions of weightlessness. However, until now, there is no coherent explanation for these observations, and little information is available concerning the biomolecules involved. Our aim has been to investigate the protein pattern of Xenopus laevis embryos exposed to simulated microgravity during the first 6 days of development. A proteomic approach was applied to compare the protein profiles of Xenopus embryos developed in simulated microgravity and in normal conditions. Attention was focused on embryos that do not present visible malformations in order to investigate if weightlessness has effects at protein level in the absence of macroscopic alterations. The data presented strongly suggest that some of the major components of the cytoskeleton vary in such conditions. Three major findings are described for the first time: (i) the expression of important factors involved in the organization and stabilization of the cytoskeleton, such as Arp (actin-related protein) 3 and stathmin, is heavily affected by microgravity; (ii) the amount of the two major cytoskeletal proteins, actin and tubulin, do not change in such conditions; however, (iii) an increase in the tyrosine nitration of these two proteins can be detected. The data suggest that, in the absence of morphological alterations, simulated microgravity affects the intracellular movement system of cells by altering cytoskeletal proteins heavily involved in the regulation of cytoskeleton remodelling.

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“…To understand the mechanism by which oxLDL causes dysfunction in EC, HUVEC were exposed to oxLDL at a concentration that induces cellular dysfunction. Results showed that nucleophosmin, stathmin, and nucleolin were differentially expressed after exposure to oxLDL [68]. Caveolae and rafts are specialized microdomains of the EC plasma membrane, which play an important role in signal transduction, transcellular transport, and cholesterol homeostasis.…”

Section: Ecmentioning

confidence: 99%

Vascular proteomics

Vivanco

Mas

Dardé

et al. 2007

Proteomics Clinical Apps

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The characterization of patients with acute coronary syndromes (ACS) at the molecular and cellular levels provides a novel vision for understanding the pathological and clinical expression of the disease. Recent advances in proteomic technologies permit the evaluation of systematic changes in protein expression in many biological systems and have been extensively applied to cardiovascular diseases (CVD). The cardiovascular system is in permanent intimate contact with blood, making blood-based biomarker discovery a particularly worthwhile approach. Thus, proteomics can potentially yield novel biomarkers reflecting CVD, establish earlier detection strategies, and monitor response to therapy. Here we review the different proteomic strategies used in the study of atherosclerosis and the novel proteins differentially expressed and secreted by atherosclerotic lesions which constitute novel potential biomarkers (HSP-27, Cathepsin D). Special attention is paid to MS-Imaging of atheroma plaque and the generation, for the first time, of 2-D images of lipids, showing the distribution of these molecules in the different areas of the atherosclerotic lesions. In addition new potential biomarkers have been identified in plasma (amyloid A1a, transtherytin), circulating cells (protein profile in monocytes from ACS patients) and individual cells constituents of atheroma plaques (endothelial, VSMC, macrophages) which provide novel insights into vascular pathophysiology.

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Proteomic characterization of oxidative dysfunction in human umbilical vein endothelial cells (HUVEC) induced by exposure to oxidized LDL

Cited by 35 publications

References 35 publications

Stressing on the nucleolus in cardiovascular disease

Stressing on the nucleolus in cardiovascular disease

Protein pattern ofXenopus laevisembryos grown in simulated microgravity

Vascular proteomics

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