Proteomic Characterization of Midproliferative and Midsecretory Human Endometrium

Chen, Jenny I C; Hannan, Natalie J.; Mak, Yunxian; Nicholls, Peter K.; Zhang, Jin; Rainczuk, Adam; Stanton, Peter G.; Robertson, David; Salamonsen, Lois A.; Stephens, Andrew N.

doi:10.1021/pr801024g

Cited by 101 publications

(100 citation statements)

References 41 publications

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“…Of the hundreds of gene expression changes typically identified by microarray, relatively few are common to more than two studies [47,48]. Comparison of proteomic data with published gene expression data in similar cohorts of women [47,49,50] also have revealed an overall lack of correlation between the two, suggesting that posttranscriptional or translational regulation is an important feature in human biology.…”

Section: Discussionmentioning

confidence: 99%

Protein expression in human cumulus cells as an indicator of blastocyst formation and pregnancy success

Braga

Setti

Turco

et al. 2016

J Assist Reprod Genet

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Purpose The goal for the present study was to implement a technique for protein extraction and identification in human cumulus cells (CCs). Methods Forty samples of CCs were collected after ovum pick-up from patients undergoing intracytoplasmic sperm injection (ICSI). Samples were split into the blastocyst group (n = 10), including patients in which all embryos converted into blastocysts, and the non-blastocyst group (n = 10), including patients in which none of the embryos reached the blastocyst stage or the positive-pregnancy (n = 10) and negativepregnancy group (n = 10). Proteins were extracted and injected into a liquid chromatography system coupled to a mass spectrometer. The spectra were processed and used to search a database. Results There were 87 different proteins in samples from the blastocyst and non-blastocyst groups, in which 30 were exclusively expressed in the blastocyst group and 17 in the nonblastocyst group. Among the 72 proteins detected in the pregnancy groups, 19 were exclusively expressed in the positive, and 16 were exclusively expressed in the negative-pregnancy group. Conclusions CC proteomics may be useful for predicting pregnancy success and the identification of patients that should be included in extended embryo culture programs.

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Section: Discussionmentioning

confidence: 99%

Protein expression in human cumulus cells as an indicator of blastocyst formation and pregnancy success

Braga

Setti

Turco

et al. 2016

J Assist Reprod Genet

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“…This is due to posttranscriptional regulatory mechanisms (mRNA export, surveillance, silencing and turnover) and posttranslational modifications, all of which can determine protein activity, localisation, turnover and interactions with other proteins (Mann & Jensen 2003). Several studies have previously sought to identify proteins differentially expressed in the human endometrium between the proliferative and secretory phases of normal menstrual cycles (DeSouza et al 2005, Chen et al 2009, Domínguez et al 2009, Parmar et al 2009, Rai et al 2010. There are also a number of studies describing endometrial phasespecific transcriptomic profiles (Kao et al 2002, Ace & Okulicz 2004, Evans et al 2012, Garrido-Gómez et al 2013.…”

Section: Introductionmentioning

confidence: 99%

Proteomic analysis of the sheep caruncular and intercaruncular endometrium reveals changes in functional proteins crucial for the establishment of pregnancy

Al-Gubory¹,

Arianmanesh²,

Garrel³

et al. 2014

Reproduction

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The expression and regulation of endometrial proteins are crucial for conceptus implantation and development. However, little is known about site-specific proteome profiles of the mammalian endometrium during the peri-implantation period. We utilised a two-dimensional gel electrophoresis/mass spectrometry-based proteomics approach to compare and identify differentially expressed proteins in sheep endometrium. Caruncular and intercaruncular endometrium were collected on days 12 (C12) and 16 (C16) of the oestrous cycle and at three stages of pregnancy corresponding to conceptus pre-attachment (P12), implantation (P16) and post-implantation (P20). Abundance and localisation changes in differentially expressed proteins were determined by western blot and immunohistochemistry. In caruncular endometrium, 45 protein spots (5% of total spots) altered between day 12 of pregnancy (P12) and P16 while 85 protein spots (10% of total spots) were differentially expressed between P16 and C16. In intercaruncular endometrium, 31 protein spots (2% of total spots) were different between P12 and P16 while 44 protein spots (4% of total spots) showed differential expression between C12 and C16. The pattern of protein changes between caruncle and intercaruncle sites was markedly different. Among the protein spots with implantation-related changes in volume, 11 proteins in the caruncular endometrium and six proteins in the intercaruncular endometrium, with different functions such as protein synthesis and degradation, antioxidant defence, cell structural integrity, adhesion and signal transduction, were identified. Our findings highlight the different but important roles of the caruncular and intercaruncular proteins during early pregnancy.

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“…These proteins were found to be localized in the luminal epithelium of endometrium, thereby strengthening the possibility of their secretion from the endometrium into the uterine cavity. Rat uterine secretome and its modulation Interestingly, in humans also, expression of endometrial b-actin has been reported to be upregulated in the receptive phase (Chen et al 2009). In the same study, expression of endometrial Rho-GDIa was also found to be higher in the receptive phase.…”

Section: Discussionmentioning

confidence: 99%

Uterine secretome and its modulation in rat (Rattus norvegicus)

et al. 2013

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The present study identifies uterine fluid (UF) proteins that display differential abundance during the embryo-permissive phase in nonconception and conception cycles in rats. UF samples were collected from nonpregnant rats in the proestrous (nZ17) and metestrous (nZ18) phases and also from pregnant (nZ17) and pseudopregnant (nZ17) rats on day 4 post coitus. UF protein profile in the metestrous phase was compared with that in the proestrous phase. Similarly, UF protein profile of the pregnant rats was compared with that of the pseudopregnant rats. Two-dimensional PAGE, followed by densitometric analysis of the paired protein spots, revealed differential abundance of 44 proteins in the metestrous phase, compared with that in the proestrous phase. Of these, 29 proteins were identified by matrix-assisted laser desorption/ionization time-of-flight or liquid chromatography-tandem mass spectrometry. Functional groups such as proteases, protease inhibitors, and oxidoreductases were enriched in differentially abundant proteins. Total protease activity in UF was found to be significantly (P!0.05; t-test) higher in the proestrous phase, compared with that in the metestrous phase. Furthermore, 41 UF proteins were found to be differentially abundant in pregnant rats, compared with pseudopregnant rats. Of these, 11 proteins could be identified. Immunoblotting analysis confirmed significantly higher (P!0.05; t-test) abundance of b-actin, Rho-specific guanine nucleotide dissociation inhibitor alpha (Rho-GDIa), and peroxiredoxin-2 and -6 in the metestrous phase, compared with that in the proestrous phase. Compared with pseudopregnant rats, pregnant rats had significantly higher (P!0.05; t-test) levels of UF b-actin and Rho-GDIa. Furthermore, these proteins could be detected in the culture supernatants of endometrial epithelial cell lines, thereby providing an evidence of their secretion from endometrial epithelial cells. Data obtained from the study expand our knowledge on the uterine milieu that favours embryo implantation.

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Proteomic Characterization of Midproliferative and Midsecretory Human Endometrium

Cited by 101 publications

References 41 publications

Protein expression in human cumulus cells as an indicator of blastocyst formation and pregnancy success

Protein expression in human cumulus cells as an indicator of blastocyst formation and pregnancy success

Proteomic analysis of the sheep caruncular and intercaruncular endometrium reveals changes in functional proteins crucial for the establishment of pregnancy

Uterine secretome and its modulation in rat (Rattus norvegicus)

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