Proteomic characterization of Aspergillus fumigatus isolated from air and surfaces of the International Space Station

Blachowicz, Adriana; Chiang, Abby J.; Romsdahl, Jillian; Kalkum, Markus; Wang, Clay C. C.; Venkateswaran, Kasthuri

doi:10.1016/j.fgb.2019.01.001

Cited by 30 publications

(24 citation statements)

References 91 publications

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“…Toxins 2020, 12,7 3 of 26 from a terpene and contains another epoxide. These epoxides are in part responsible for the instability of the molecule.…”

Section: Fumagillin Physichochemical Propertiesmentioning

confidence: 99%

“…Aspergillus fumigatus is a saprophytic mold that plays an important ecological role as a decomposer, recycling carbon and nitrogen sources [1][2][3]. It is a ubiquitous fungus with a worldwide distribution, which can be detected in air and soil samples, and even on the International Space Station [4][5][6][7]. This ubiquity is because it is highly adaptive; able to colonize a wide range of environments because of its metabolic diversity, broad stress, and thermal tolerances; and has the ability to spread its conidia easily [4,6,8,9].…”

Section: Introductionmentioning

confidence: 99%

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Fumagillin, a Mycotoxin of Aspergillus fumigatus: Biosynthesis, Biological Activities, Detection, and Applications

Guruceaga

Perez-Cuesta

Abad‐Diaz‐de‐Cerio

et al. 2019

Toxins

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Fumagillin is a mycotoxin produced, above all, by the saprophytic filamentous fungus Aspergillus fumigatus. This mold is an opportunistic pathogen that can cause invasive aspergillosis, a disease that has high mortality rates linked to it. Its ability to adapt to environmental stresses through the production of secondary metabolites, including several mycotoxins (gliotoxin, fumagillin, pseurotin A, etc.) also seem to play an important role in causing these infections. Since the discovery of the A. fumigatus fumagillin in 1949, many studies have focused on this toxin and in this review we gather all the information currently available. First of all, the structural characteristics of this mycotoxin and the different methods developed for its determination are given in detail. Then, the biosynthetic gene cluster and the metabolic pathway involved in its production and regulation are explained. The activity of fumagillin on its target, the methionine aminopeptidase type 2 (MetAP2) enzyme, and the effects of blocking this enzyme in the host are also described. Finally, the applications that this toxin and its derivatives have in different fields, such as the treatment of cancer and its microsporicidal activity in the treatment of honeybee hive infections with Nosema spp., are reviewed. Therefore, this work offers a complete review of all the information currently related to the fumagillin mycotoxin secreted by A. fumigatus, important because of its role in the fungal infection process but also because it has many other applications, notably in beekeeping, the treatment of infectious diseases, and in oncology. Key Contribution:Fumagillin is a mycotoxin produced during infections caused by A. fumigatus and in the adaptation processes of this species to multiple environmental stresses. In this review; we include detailed information on the fungal mechanisms employed in the production of this mycotoxin and those present in its regulation, how it acts on its target, and how it can be detected, all of which may be useful for diagnosis or the development of new treatments. Its usefulness in different fields is also described; because of its anti-angiogenic activity it is used to attack different types of tumors; at the same time its antibiotic activity is useful against several parasites.

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“…Toxins 2020, 12,7 3 of 26 from a terpene and contains another epoxide. These epoxides are in part responsible for the instability of the molecule.…”

Section: Fumagillin Physichochemical Propertiesmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Fumagillin, a Mycotoxin of Aspergillus fumigatus: Biosynthesis, Biological Activities, Detection, and Applications

Guruceaga

Perez-Cuesta

Abad‐Diaz‐de‐Cerio

et al. 2019

Toxins

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“…It is admitted today that fungal spores are very sensitive to temperature and water (e.g., relative humidity and rain for their proper development [15]. It is the case on Earth but also in confined and much stressed environments like the International Space Station [68].…”

Section: Interannuality and Global Increase Of Specific Afsin Concentmentioning

confidence: 99%

Variability and Geographical Origin of Five Years Airborne Fungal Spore Concentrations Measured at Saclay, France from 2014 to 2018

et al. 2019

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Airborne fungal spores (AFS) represent the major fraction of primary biological aerosol particles (PBAPs), and they are studied worldwide largely due to their important role within the Earth system. They have an impact on climate and human health, and they contribute to the propagation of diseases. As their presence in the air depends largely on studied ecosystems, a spore trap was used to monitor their atmospheric concentrations from 2014 to December 2018 in Saclay, a suburban area in the megacity of Paris. The main objective of this work was: (1) to understand the atmospheric variability of AFS in relation to different variables such as meteorological factors, agricultural practice, and (2) to identify their geographical origin by using a source receptor model. During our period of observation, 30 taxa have been identified under a light microscope. In order of importance, Ascospores, Cladosporium, Basidiospores, Tilletiopsis, Alternaria were found to be the most abundant types respectively (50.8%, 33.6%, 7.6%, 1.8%, and 1.3%) accounting for 95% of the atmospheric concentrations. We observed a general decrease associated with a strong interannual variability. A bimodal seasonal cycle was identified with a first maximum in July and a second in October. The main parameters driving the atmospheric concentration are temperature and precipitation. The daily variability is strongly activated by successive periods of hot weather and rainfall, multiplying the concentration by a factor of 1000 in less than 12 hours. Results from the source receptor model ZeFir point out unambiguous different origins of AFS due to specific sources impacting the observation site. Our study also indicated that a hydrological stress has a direct effect on the daily concentrations. This last point should be taken into account for every stressed ecosystem studied in a global warming context. This is particularly important for Mediterranean areas where water is a key control of the growth and dispersion of fungal spores.

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“…These studies include the synthesis of pigments by Monascus purpureus under high ammonium chloride concentration (Zhou et al, 2020), the polyextremophilic bacterium Deinococcus radiodurans in response to oxidative stress (Gao et al, 2020), the pigmentation factors in Pseudomonas fluorescens ITEM 17298 (Quintieri et al, 2019), and the air-isolated Aspergillus sp. from International Space Station (Blachowicz et al, 2019).…”

Section: Proteomics and Metabolomicsmentioning

confidence: 99%

Natural Pigments of Microbial Origin

Pailliè-Jiménez

Stincone

Brandelli

2020

Front. Sustain. Food Syst.

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The world demands new solutions and products to be used as dyes for industrial applications. Microbial pigments represent an eco-friendly alternative as they can be produced in large amounts through biotechnological processes and do not present environmental risks, as they are easily decomposable. Moreover, some of these metabolites are recognized for their biological activities, which qualify them for potential uses as food colorants and nutraceuticals, protecting against degenerative diseases related with oxidative stress. Because of their genetic simplicity as compared with plants, microorganisms may be a better source to understand biosynthetic mechanisms and to be engineered for producing high pigment yields. Despite the origin of the pigmented microorganism, it seems very important to develop protocols using organic industrial residues and agricultural byproducts as substrates for pigment production and find novel green strategies for rapid pigment extraction. This review looks for the most recent studies that describe microbial pigments from microalgae, fungi, and bacteria. In particular, the underexploited tools of omics science such as proteomics and metabolomics are addressed. The use of techniques involving mass spectrometry, allows to identify different protein and metabolite profiles that may be associated with a variety of biotechnologically-relevant pathways of pigment synthesis.

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Proteomic characterization of Aspergillus fumigatus isolated from air and surfaces of the International Space Station

Cited by 30 publications

References 91 publications

Fumagillin, a Mycotoxin of Aspergillus fumigatus: Biosynthesis, Biological Activities, Detection, and Applications

Fumagillin, a Mycotoxin of Aspergillus fumigatus: Biosynthesis, Biological Activities, Detection, and Applications

Variability and Geographical Origin of Five Years Airborne Fungal Spore Concentrations Measured at Saclay, France from 2014 to 2018

Natural Pigments of Microbial Origin

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