Proteomic Characterization of a New asymmetric Cellulose Triacetate Membrane for Hemodialysis

Ronci, Maurizio; Leporini, Lidia; Felaco, Paolo; Sirolli, Vittorio; Pieroni, Luisa; Greco, Viviana; Aceto, Antonio; Urbani, Andrea; Bonomini, Mario

doi:10.1002/prca.201700140

Cited by 12 publications

(10 citation statements)

References 44 publications

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“…The mass spectrometry analysis carried out on the pOMV and bOMV lysates showed that the hydratase activity was due to the periplasmic bacterial α-CA, indicated with the acronym hpαCA. Further studies will be necessary to elucidate the role or the fate of α-CA delivered by OMVs in planktonic and biofilm phenotypes as well as a possible involvement of α-CA in eDNA release as previously demonstrated for nontuberculous mycobacteria 44 . However, these results shed new light in the field of the strategies used to combat the microbial infections.…”

Section: Resultsmentioning

confidence: 82%

“…In-source reference lock mass (1221.9906 m/z ) was acquired online throughout the runs. The raw data were processed using PEAKS Studio v7.5 software (Bioinformatic Solutions Inc, Waterloo, Canada) using the function “correct precursor only” as already reported 44 . Briefly, the mass lists were searched against a custom database containing H. pylori NCTC11637 proteins and a list of common contaminants (June 2017; 1880 entries) using 10 ppm and 0.05 Da as the highest error tolerances for parent and fragment ions, respectively.…”

Section: Methodsmentioning

confidence: 99%

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Identification and characterization of the α-CA in the outer membrane vesicles produced byHelicobacter pylori

Ronci¹,

Prete

Puca

et al. 2019

Journal of Enzyme Inhibition and Medicinal Chemistry

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The genome of Helicobacter pylori encodes for carbonic anhydrases (CAs, EC 4.2.1.1) belonging to the α- and β-CA classes, which together with urease, have a pivotal role in the acid acclimation of the microorganism within the human stomach. Recently, in the exoproteome of H. pylori, a CA with no indication of the corresponding class was identified. Here, using the protonography and the mass spectrometry, a CA belonging to the α-class was detected in the outer membrane vesicles (OMVs) generated by planktonic and biofilm phenotypes of four H. pylori strains. The amount of this metalloenzyme was higher in the planktonic OMVs (pOMVs) than in the biofilm OMVs (bOMVs). Furthermore, the content of α-CA increases over time in the pOMVs. The identification of the α-CA in pOMVs and bOMVs might shed new light on the role of this enzyme in the colonization, survival, persistence, and pathogenesis of H. pylori.

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Section: Resultsmentioning

confidence: 82%

Section: Methodsmentioning

confidence: 99%

Identification and characterization of the α-CA in the outer membrane vesicles produced byHelicobacter pylori

Ronci¹,

Prete

Puca

et al. 2019

Journal of Enzyme Inhibition and Medicinal Chemistry

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“…In comparison with a conventional symmetric membrane, the modified cellulose triacetate (CTA) for the biocompatible AM was achieved by the basic mechanism of blood interaction on a smooth surface. The novel CTA, strengthened by the physio-chemical characteristics of the blood-exposed membrane surface, together with important properties of the CAM, could further lead to progressive remedies of hemodialysis [54].…”

Section: Hemodialysismentioning

confidence: 99%

Asymmetric Cellulosic Membranes: Current and Future Aspects

et al. 2020

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In this paper, we report our attempt to elaborate on cellulose-based materials and their potential application in membrane science, especially in separation applications. Furthermore, the cellulosic membrane has received attention for potential use as biomaterials such as novel wound-dressings and hemodialysis materials. In this mini-review, we mainly focus on the separation and antimicrobial properties of cellulosic membranes and the advanced synthesis/processing methods for superior functional quality for various potential applications. Finally, we conclude with the market and the impact of developments of future expectations.

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“…Another important property of CTA is its low thrombogenetic potential. 14 The asymmetric triacetate membrane (ATA), which was created by modifying CTA, is a new compound developed by the Nipro Corporation (Osaka, Japan). Its main properties are its asymmetrical structure and smooth surface, resulting in high permeability during massive filtration, less variation in permeation time, better biocompatibility, and lower protein adsorption.…”

Section: Chemical Composition and Its Influence On Membrane Performancementioning

confidence: 99%

Dialysis membranes: A 2018 update

Olczyk¹,

Małyszczak²,

Kusztal³

2018

Polim. Med.

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Dialysis membranes are the basic element of a hemodialyzer. Synthetic and natural materials characterized by various fiber arrangements are used in their production. The most up-to-date ones are made of synthetic polymers such as polyamide, phosphatidylserine (PS), polyacrylonitrile-based fiber (PAN), polyarylethersulfone, polyethersulfone, or polymethylmethacrylate. Dialysis membranes are characterized by the ability to remove uremic molecules, which can be divided into small water-soluble compounds, protein-bound compounds and larger "middle molecules". Newer membranes such as medium cut off membranes (MCO) allow the removal of a wider spectrum of uremic molecules, which reduces the risk of late complications of dialysis. Dialysis membranes are used in therapy methods such as low flux, high flux or HDx therapy. An important aim in dialysis membrane development is to increase their biocompatibility. Insufficient biocompatibility can result in complement activation or platelet activation, which can lead to an increased risk of cardiovascular complications. The aim of the study is to discuss the latest reports on dialysis membranes.

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Proteomic Characterization of a New asymmetric Cellulose Triacetate Membrane for Hemodialysis

Cited by 12 publications

References 44 publications

Identification and characterization of the α-CA in the outer membrane vesicles produced byHelicobacter pylori

Identification and characterization of the α-CA in the outer membrane vesicles produced byHelicobacter pylori

Asymmetric Cellulosic Membranes: Current and Future Aspects

Dialysis membranes: A 2018 update

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