Proteomic changes in Bacteroides fragilis exposed to subinhibitory concentration of piperacillin/tazobactam

Veloso, Luciano de Castro; Santos, Kênia Valéria dos; Andrade, Hélida Monteiro de; Pires, Simone da Fonseca; Santos, Simone Gonçalves dos; Trindade, Mariana; Farias, Luiz de Mácêdo; Carvalho, Maria Auxiliadora Roque de

doi:10.1016/j.anaerobe.2013.04.007

Cited by 6 publications

(7 citation statements)

References 32 publications

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“…The literature reports an increase in the time of growth of bacteria submitted to the sub-MICs of some antimicrobials and different stresses. Veloso et al (2013) observed a delay of 15 h for the beginning of the log phase of growth of B. fragilis grown in sub-MICs of PTZ, a fact that was not corroborated in our study. In addition, another study observed that Escherichia coli, when cultured in sub-MICs of PTZ, reached the peak of filamentous cells with 16 h of growth, presenting 82% of cells altered in morphology and complexity (DE ANDRADE et al, 2016).…”

Section: Discussioncontrasting

confidence: 97%

“…This may not be able to eliminate the microorganism but could interfere with the expression of some pathogenicity factors and may result in the selection of resistant bacteria. These changes in the pathogenicity of B. fragilis, in the presence of sub-MICs of antimicrobials demonstrate their pathogenic potential, being able to provoke different interactions between the host and the bacterium (DE SOUZA FILHO et al, 2012;VELOSO et al, 2013).…”

Section: Discussionmentioning

confidence: 95%

“…One reference strain (Bacteroides fragilis ATCC 25285) and two clinically isolated from patients with IAIs were evaluated (named P34 and P50). The clinical strains were isolated, identified and had their susceptibility profile stablished in a previous study (FERREIRA et al, 2016) were then incubated in anaerobic chamber at 37°C and 100 μL aliquots of each bacterial culture were removed in triplicate to read the OD at 550 nm every 3 h up to 72 h (DOS SANTOS et al, 2007;VELOSO et al, 2013;DE ANDRADE et al, 2016). Capsule Detection -The Hiss staining method was used with modifications (NAKANO et al, 2004).…”

Section: Bacterial Strainsmentioning

confidence: 99%

“…It is also able to withstand the reactive oxygen species produced by the host's defenses and persists in oxygenated tissues (TEIXEIRA et al, 2018). VELOSO et al, 2013;DE ANDRADE et al, 2016;CLSI, 2017).…”

Section: Introductionmentioning

confidence: 99%

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AVALIAÇÃO DA TOXICIDADE AGUDA E DA CITOTOXICIDADE DOS EXTRATOS ETANÓLICOS DA MACRÓFITA Hydrocotyle bonariensis LAM (APIACEAE)

Nascimento¹,

Guedes²,

Bezerra³

et al. 2020

Pesquisa Científica E Tecnológica Em Microbiologia 3

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Todo o conteúdo deste livro está licenciado sob uma Licença de Atribuição Creative Commons. Atribuição 4.0 Internacional (CC BY 4.0). O conteúdo dos artigos e seus dados em sua forma, correção e confiabilidade são de responsabilidade exclusiva dos autores. Permitido o download da obra e o compartilhamento desde que sejam atribuídos créditos aos autores, mas sem a possibilidade de alterá-la de nenhuma forma ou utilizá-la para fins comerciais.

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Section: Discussioncontrasting

confidence: 97%

Section: Discussionmentioning

confidence: 95%

Section: Bacterial Strainsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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AVALIAÇÃO DA TOXICIDADE AGUDA E DA CITOTOXICIDADE DOS EXTRATOS ETANÓLICOS DA MACRÓFITA Hydrocotyle bonariensis LAM (APIACEAE)

Nascimento¹,

Guedes²,

Bezerra³

et al. 2020

Pesquisa Científica E Tecnológica Em Microbiologia 3

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“…Sub-inhibitory concentrations of piperacillin and/or tazobactam, for instance, were recently shown to induce broad proteomic alterations in Bacteroides fragilis. 9 The persistent mutagenesis and selection environment distinct to each individual may thus constantly engineer the microbiome for optimal adaption to unique environments—the differences being observable not so much at the species level, but rather at the level of nucleotide polymorphisms.…”

mentioning

confidence: 99%

Off-label abuse of antibiotics by bacteria

Viswanathan

2014

Gut Microbes

125

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Antibiotics and antibiotic resistance made news on several fronts in the past year. Many public health organizations, including the CDC, used terms such as “crisis”, “catastrophic consequences”, and “nightmare scenario” to highlight the rapid emergence and spread of antibiotic resistance. A report from the Pew Commission on Industrial Farm Animal Production, on the fifth anniversary of the publication of its landmark 2008 report, noted that state and federal legislative efforts to limit non-therapeutic use of antibiotics in animal production were thwarted by drug and food animal industries. In its lobbying disclosures, the Farm Bureau stated that such efforts to limit use of animal antibiotics were “based on emotion and no credible peer reviewed science.” Meanwhile, there have been inexorable advances in our understanding of the molecular mechanisms by which antibiotics induce diversity and resistance in bacteria. This article reviews one study that probed the role of the bacterial general stress response in sub-inhibitory antibiotic-induced mutagenesis and antibiotic resistance.

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Sub-Inhibitory Concentration of Piperacillin–Tazobactam May be Related to Virulence Properties of Filamentous Escherichia coli

et al. 2015

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Sub-inhibitory concentrations of antibiotics are always generated as a consequence of antimicrobial therapy and the effects of such residual products in bacterial morphology are well documented, especially the filamentation generated by beta-lactams. The aim of this study was to investigate some morphological and pathological aspects (virulence factors) of Escherichia coli cultivated under half-minimum inhibitory concentration (1.0 µg/mL) of piperacillin-tazobactam (PTZ sub-MIC). PTZ sub-MIC promoted noticeable changes in the bacterial cells which reach the peak of morphological alterations (filamentation) and complexity at 16 h of antimicrobial exposure. Thereafter the filamentous cells and a control one, not treated with PTZ, were comparatively tested for growth curve; biochemical profile; oxidative stress tolerance; biofilm production and cell hydrophobicity; motility and pathogenicity in vivo. PTZ sub-MIC attenuated the E. coli growth rate, but without changes in carbohydrate fermentation or in traditional biochemical tests. Overall, the treatment of E. coli with sub-MIC of PTZ generated filamentous forms which were accompanied by the inhibition of virulence factors such as the oxidative stress response, biofilm formation, cell surface hydrophobicity, and motility. These results are consistent with the reduced pathogenicity observed for the filamentous E. coli in the murine model of intra-abdominal infection. In other words, the treatment of E. coli with sub-MIC of PTZ suggests a decrease in their virulence.

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Proteomic changes in Bacteroides fragilis exposed to subinhibitory concentration of piperacillin/tazobactam

Cited by 6 publications

References 32 publications

AVALIAÇÃO DA TOXICIDADE AGUDA E DA CITOTOXICIDADE DOS EXTRATOS ETANÓLICOS DA MACRÓFITA Hydrocotyle bonariensis LAM (APIACEAE)

AVALIAÇÃO DA TOXICIDADE AGUDA E DA CITOTOXICIDADE DOS EXTRATOS ETANÓLICOS DA MACRÓFITA Hydrocotyle bonariensis LAM (APIACEAE)

Off-label abuse of antibiotics by bacteria

Sub-Inhibitory Concentration of Piperacillin–Tazobactam May be Related to Virulence Properties of Filamentous Escherichia coli

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