Proteomic-Based Approaches for the Study of Ischemic Stroke

Hypoxia induced sex-difference in zebrafish brain proteome profile reveals the crucial role of H3K9me3 in recovery from acute hypoxia

“…However, post stroke is mostly related to protein function which can be direct target for therapeutic intervention. Therefore in the present study we performed a quantitative proteomics approach for hypoxia induced brain to identify favourable biomarkers involved in neuronal injury and recovery [10]. In our previous study clear sexspecific differences were observed in acute hypoxia-induced neural damage and recovery but to explore more about the mechanism of recovery in the present study we have focused at 4 hr post hypoxia timepoint predicting that could be possibly therapeutic window.…”

“…In our previous study clear sexspecific differences were observed in acute hypoxia-induced neural damage and recovery but to explore more about the mechanism of recovery in the present study we have focused at 4 hr post hypoxia timepoint predicting that could be possibly therapeutic window. To date, many high throughput studies on hypoxia [10,[33][34][35][36][37][38][39] gave sufficient information about the genes and proteins involved in hypoxia and related diseases but the roles of these hallmarked hypoxia markers are not well studied in a sex-specific context. Therefore, we have attempted to emphasize more on the sex-specific neural regulation post-hypoxia, which will provide a better insight into designing efficient therapeutics for patients who suffered acute hypoxic insults.…”

“…The rapid response to acute hypoxia may preclude many pathways that require many new gene expressions suggesting the mechanism underlying recovery from acute hypoxiamediated at least in part by the activities of the existing pool of mRNA and protein. An approach such as high throughput proteomic analysis is one of the ideally suited approaches to understand the neural changes induced by acute hypoxia with recovery [10].…”

“…A recent review article on Proteomics-Based Approaches for the Study of Ischemic Stroke [10] discussed the proteomics study of ischemic stroke using in vivo and in vitro models, with and without interventions and taking tissue, cerebrospinal fluid or plasma. Although proteomic studies have contributed with a long list of potential biomarkers for diagnosis, prognosis, and monitoring of ischemic stroke, most of these have not been implemented in clinical application successfully.…”

Hypoxia Induced Sex-Difference in Zebrafish Brain Proteome Profile Reveals the Crucial Role of H3K9me3 in Recovery From Acute Hypoxia

“…An approach such as high throughput proteomic analysis is one of the ideally suited approaches to understand the neural changes induced by acute hypoxia with recovery (Li et al, 2019).…”