Proteomic approaches in microalgae: perspectives and applications

Abstract

Biofuels are the promising sources which are produced by various microalgae or in the form of metabolic by-products from organic or food waste products. Microalgae have been widely reported for the production of biofuels since these have a high storage of lipids as triacylglycerides, which can mainly be converted into biofuels. Recently, products such as biodiesel, bioethanol and biogas have renewed the interest toward the microalgae. The proteomics alone will not pave the way toward finding an ideal alga which will fulfill the current energy demands, but a combined approach of proteomics, genomics and bioinformatics can be pivotal for a sustainable solution. The present review emphasizes various technologies currently involved in algal proteomics for the efficient production of biofuels.

Morpho-taxonomic, genetic, and biochemical characterization of freshwater microalgae as potential biodiesel feedstock

“…They were found as single cells as well as in irregularly arranged colonies. The characteristic features observed in NEIST BT-10 are common to genus Desmodesmus (An et al 1999). The morphological and colonial characteristics of NEIST BT-A1 showed resemblance with that of NEIST BT-A10.…”

“…Thus, to use algae as an efficient alternative energy source for biodiesel production, the challenge remains to increase both lipid productivity and biomass concentration. Meanwhile, there have been other efforts to increase biomass production as well as the lipid content of a microalgae through various genomic and proteomic approaches that will uncover the biological implications and pave the future tailored manipulation of microalgae for broader industrial applications (Anand et al 2017;Banerjee et al 2017;Li et al 2019). However, for any kind of modifications to be made at the molecular level, the first step is to screen microalgae strains from different habitats and examine them for their inherent properties to be used as an ideal strain for biodiesel production (Jagadevan et al 2018).…”

Advances and challenges in genetic engineering of microalgae

“…Such modern experimentation tools balance the research gaps and offer better understanding of proteomic‐based approaches in microalgae (Anand et al . ).…”

“…; Arruda ; Anand et al . ). The current technologies have enabled the manipulation of one or more genes to study system‐based function and other molecular applications.…”

Recent developments in synthetic biology and metabolic engineering in microalgae towards biofuel production

“…Amongst various biomass, microalgae are gaining much attention for use as a potential feedstock by virtue of its high carbohydrate and lipid content, rapid growth rate and resistance to fluctuating environmental conditions [ 6 ]. Advancement in reverse engineering tools such as genome sequence, genetic transformation, gene targeting, different promoters, selection markers and molecular biology techniques such as clustered regularly interspaced short palindromic repeats (CRISPR), transcription activator-like effectors (TALEs) and zinc-finger nucleases (ZFN) have paved the way to unravel novel metabolic pathways occurring within the algal cells or to design and synthesize new biological systems, all of which are aimed at furthering biofuel production [ 7 – 10 ]. Figure 1 illustrates the overall process involved in microalgae-based biofuel production.…”