Proteomic approach to understand the molecular physiology of symbiotic interaction between Piriformospora indica and Brassica napus

Abstract

Many studies have been now focused on the promising approach of fungal endophytes to protect the plant from nutrient deficiency and environmental stresses along with better development and productivity. Quantitative and qualitative protein characteristics are regulated at genomic, transcriptomic, and posttranscriptional levels. Here, we used integrated in-depth proteome analyses to characterize the relationship between endophyte Piriformospora indica and Brassica napus plant highlighting its potential involvement in symbiosis and overall growth and development of the plant. An LC-MS/MS based label-free quantitative technique was used to evaluate the differential proteomics under P. indica treatment vs. control plants. In this study, 8,123 proteins were assessed, of which 46 showed significant abundance (34 downregulated and 12 upregulated) under high confidence conditions (p-value ≤ 0.05, fold change ≥2, confidence level 95%). Mapping of identified differentially expressed proteins with bioinformatics tools such as GO and KEGG pathway analysis showed significant enrichment of gene sets involves in metabolic processes, symbiotic signaling, stress/defense responses, energy production, nutrient acquisition, biosynthesis of essential metabolites. These proteins are responsible for root’s architectural modification, cell remodeling, and cellular homeostasis during the symbiotic growth phase of plant’s life. We tried to enhance our knowledge that how the biological pathways modulate during symbiosis?

Heterodera schachtii infection affects nitrogen metabolism in Arabidopsis thaliana

“…Secondly, glutamate is not only important for nematodes but also for the colonized plants. During the biotic interaction, the proteome of the plant is deeply remodelled (Shrivastava et al , ), and glutamate is the hub of the amino acid metabolism. Therefore, the increased GDH activity in both roots and shoots of infected plants at 3 and 7 dpi, observed in the present study, can stimulate defence responses by providing glutamate for the synthesis of key defence amino acids (γ‐aminobutyric acid, arginine, and proline; Seifi et al , ; Labudda et al , ) as well as proteins, which participate in plant stress responses (Muñoz‐Nortes et al , ).…”

Understanding Host–Pathogen Interactions in Brassica napus in the Omics Era

“…In a proteomic study applied to a non-pathogenic, arbuscular mycorrhizal fungal species, Piriformospora indica , studying the beneficial effect of the fungal endophyte on the B. napus host, liquid chromatography-mass spectrometry (LC-MS), coupled with bioinformatics, highlighted significant levels of differentially expressed proteins involved in the stress/defence response during the cell-death colonisation phase in the plant roots, elucidating the role of P. indica in enhancing B. napus resistance against abiotic and biotic stresses [ 159 ]. There is opportunity for the further characterisation of the genes that encode the stress-response proteins expressed in the B. napus–P.…”

Plant–Microbe Symbiosis: What Has Proteomics Taught Us?

“…One of the well‐characterized root‐endophytic fungi is Serendipita indica (formerly known as Piriformospora indica ), which has received great attention over recent decades owing to its exceptional ability to efficiently promote plant growth and confer stress tolerance to different host plants under both salinity and drought stress . Shrivastava et al . used a proteomic approach to characterize the interaction between S. indica and Brassica napus roots .…”

“…Shrivastava et al . used a proteomic approach to characterize the interaction between S. indica and Brassica napus roots . Out of 8123 quantified proteins only 46 displayed a significant change in abundance in response to S indica colonization.…”