Proteomic Approach to Screen Peanut Genotypes with Enhanced Nutritional Qualities

Ramesh, Katam; Vasanthaiah, Hemanth K. N.; Basha, Sheikh M.

doi:10.1109/fbit.2007.94

Cited by 3 publications

(3 citation statements)

References 27 publications

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“…Until now, 2-DE proteomic studies of peanut have enabled identification and characterization of seed proteins from cultivated peanut and identification of genetic variation among peanut cultivars [28,29]. Proteomics has been used to successfully identify diverse seed storage proteins, various aspects of leaf development, and genetic diversity in sub species of Arachis [30,31].…”

Section: Introductionmentioning

confidence: 99%

Comparative leaf proteomics of drought-tolerant and -susceptible peanut in response to water stress

Katam

Sakata

Suravajhala³

et al. 2016

Journal of Proteomics

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Peanuts (Arachis hypogaea L.) are a major source of plant protein grown in subtropical and tropical regions of the world. Pre-harvest aflatoxin contamination is a major problem that affects peanut crop yield and food safety. Poor understanding of molecular and cellular mechanisms associated with aflatoxin resistance is largely responsible for the lack of progress in elucidating a process/methodology for reducing aflatoxin contamination in peanuts. Drought perturbs the invasion of the aflatoxin producing fungus and thus affects the quality and yield of peanut. Therefore, more studies involving the effects of drought stress to determine the molecular changes will enhance our understanding of the key metabolic pathways involved in the combined stresses. The changes associated with the biotic and abiotic interactions within the peanut will be used to determine the metabolic pathways involved in the stress tolerance. This research would be beneficial in identifying the tolerant molecular signatures and promoting food safety and consumer health through breeding superior quality peanut cultivars.

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Section: Introductionmentioning

confidence: 99%

Comparative leaf proteomics of drought-tolerant and -susceptible peanut in response to water stress

Katam

Sakata

Suravajhala³

et al. 2016

Journal of Proteomics

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“…30) The synthesis of a number of peanut seed functional proteins including defensive proteins could be up-regulated according to water stress, A. flavus infection, or aflatoxin contamination. 11,12,[43][44][45] Basically, these proteins are less abundant and natively hydrophilic so that they should not be confound in the prolamin fractions after the two-step extraction used in the study.…”

Section: Discussionmentioning

confidence: 99%

“…The use of drought-sensitive/ resistant genotypes together with water-deficit treatment has been the conventional tool to investigate total protein extract for protein markers of the resistance traits to drought and seed aflatoxin contamination in peanuts. 12,44) That no correspondence was found between the prolamin content and the resistance traits might be resulted from the fractionated extraction procedure. The present data revealed the existence of prolamins with positive test in inhibiting A. flavus spore germination.…”

Section: Discussionmentioning

confidence: 99%

Identification of peanut seed prolamins with an antifungal role by 2D-GE and drought treatment

Senakoon

Nuchadomrong

Chiou

et al. 2015

Bioscience, Biotechnology, and Biochemistry

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This work revealed peanut seed prolamins likely displaying a defensive role besides the known nitrogen storage. Drought stress and proteomic approaches were used in varieties of peanuts to explore the prolamin member in association with a test against Aspergillus flavus spore germination. The stress effect was showed by aerial biomass, leaf content of malondialdehyde, and seed contamination by A. flavus. Sodium dodecyl sulfate–polyacrylamide gel electrophoresis profiles were not informative for the antifungal polypeptides. From two-dimensional gel electrophoresis, the suspected polypeptides were those with pI 5.45–5.75 and sizes of 22.0–30.5 kDa specifically in Spanish-type peanuts. Regarding to the drought effect in most of these peanuts, the spot peak volume analysis deduced three novel prolamin-related antifungal polypeptides at pI 5.75–5.8 with 30.5, 27.5–28.5, and 22.0–22.5 kDa, which was confirmed after isoelectric purification at pH 5.60. The data could not yet conclude their correlation with resistance to drought and to seed infection by A. flavus.

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An Alternate Fractionation of Peanut Seed Proteins in Association with Inhibitory Assay on Aspergillus flavus

et al. 2012

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Proteomic Approach to Screen Peanut Genotypes with Enhanced Nutritional Qualities

Cited by 3 publications

References 27 publications

Comparative leaf proteomics of drought-tolerant and -susceptible peanut in response to water stress

Comparative leaf proteomics of drought-tolerant and -susceptible peanut in response to water stress

Identification of peanut seed prolamins with an antifungal role by 2D-GE and drought treatment

An Alternate Fractionation of Peanut Seed Proteins in Association with Inhibitory Assay on Aspergillus flavus

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