Proteomic and transcriptomic studies of BGC823 cells stimulated with Helicobacter pylori isolates from gastric MALT lymphoma

Zhou, Qinghua; Zhang, Huifang; Meng, Fanliang; He, Lihua; Zhang, Jianzhong; Xiao, Di

doi:10.1371/journal.pone.0238379

Cited by 5 publications

(4 citation statements)

References 51 publications

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“…A total of 32 H. pylori strains (2 isolated from GML patients, 10 each isolated from gastritis patients, gastric ulcer patients and gastric cancer patients) from the H. pylori strain library of the China CDC were used in this study. We performed both transcriptomic and proteomic analyses on gastric cancer cells infected by the two H. pylori isolates from GML patients and the reference strain 26695 in our previous studies, and found specific differentially expressed genes and differentially expressed proteins induced by the GML isolates ( Zou et al., 2020 ). The H. pylori strains were identified by microscopic examination and urease, catalase, and oxidase activity tests.…”

Section: Methodsmentioning

confidence: 99%

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Glycopeptidomics Analysis of a Cell Line Model Revealing Pathogenesis and Potential Marker Molecules for the Early Diagnosis of Gastric MALT Lymphoma

Xiao

Zhou

Meng

et al. 2021

Front. Cell. Infect. Microbiol.

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Background & AimsGastric mucosa-associated lymphoma (GML) is a mature B cell tumor related to Helicobacter pylori (H.pylori) infection. The clinical manifestations of GML are not specific, so GML is often misdiagnosed, leading to excessive treatment. The pathogenesis of H.pylori-induced GML is not well understood and there are no molecular markers for early GML diagnosis.MethodsGlycopeptidomics analyses of host cell lines (a BCG823 cell line, C823) and C823 cells infected by H. pylori isolated from patients with GML (GMALT823), gastritis (GAT823), gastric ulcer (GAU823) and gastric cancer (GAC823) were carried out to clarify the host reaction mechanism against GML and to identify potential molecular criteria for the early diagnosis of GML.ResultsThirty-three samples were analyzed and approximately 2000 proteins, 200 glycoproteins and 500 glycopeptides were detected in each sample. O-glycans were the dominant glycoforms in GMALT823 cells only. Four specific glycoforms in GMALT823 cells and 2 specific glycoforms in C823 and GMALT823 cells were identified. Eight specific glycopeptides from 7 glycoproteins were found in GMALT823 cells; of these glycopeptides, 6 and 3 specific glycopeptides had high affinity for T cell epitopes and have conformational B cell epitopes, respectively.ConclusionThe predominant glycoforms of host cells infected by MALT H. pylori isolates differ from others, and the glycoproteins, glycosylation sites and glycoforms might be closely related to the formation of GML, which provides new insights into the pathogenic mechanisms of H. pylori infection and suggests molecular indicators for the early diagnosis of GML.

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Section: Methodsmentioning

confidence: 99%

“…In this study, we used a human gastric carcinoma undifferentiated cell line BCG823 which have been used in previous studies to reveal the proteome variation infected by MALT isolates and other isolates ( Zou et al., 2020 ). The cell line was cultured in RPMI 1640 supplemented with 10% FBS at 37°C in an atmosphere of 5% CO 2 .…”

Section: Methodsmentioning

confidence: 99%

Glycopeptidomics Analysis of a Cell Line Model Revealing Pathogenesis and Potential Marker Molecules for the Early Diagnosis of Gastric MALT Lymphoma

Xiao

Zhou

Meng

et al. 2021

Front. Cell. Infect. Microbiol.

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“…Various biomedical in vivo systems have demonstrated the existence of Hp biofilms in gastric biopsies. Recent transcriptomic and proteomic analyses have provided valuable insights into the transition from planktonic Hp to the biofilm phase, including higher gene expression of adhesins, flagella, toxins, efflux pumps, lipopolysaccharides (LPS), the type IV secretion system, urease, and hydrogenase, for obtaining alternative energy sources [ 56 ]. Conversely, factors involved in quorum sensing, metabolism, and translation are reduced in biofilms [ 57 ].…”

Section: Biofilm-associated Helicobacter Pylori An...mentioning

confidence: 99%

Biofilm and Cancer: Interactions and Future Directions for Cancer Therapy

Choi

Murray

Choi

2023

IJMS

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There is a growing body of evidence supporting the significant role of bacterial biofilms in the pathogenesis of various human diseases, including cancer. Biofilms are polymicrobial communities enclosed within an extracellular matrix composed of polysaccharides, proteins, extracellular DNA, and lipids. This complex matrix provides protection against antibiotics and host immune responses, enabling the microorganisms to establish persistent infections. Moreover, biofilms induce anti-inflammatory responses and metabolic changes in the host, further facilitating their survival. Many of these changes are comparable to those observed in cancer cells. This review will cover recent research on the role of bacterial biofilms in carcinogenesis, especially in colorectal (CRC) and gastric cancers, emphasizing the shared physical and chemical characteristics of biofilms and cancer. This review will also discuss the interactions between bacteria and the tumor microenvironment, which can facilitate oncogene expression and cancer progression. This information will provide insight into developing new therapies to identify and treat biofilm-associated cancers, such as utilizing bacteria as delivery vectors, using bacteria to upregulate immune function, or more selectively targeting biofilms and cancer for their shared traits.

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“…By deregulating these pathways, H. pylori might influence gastric lymphomagenesis through modulating the proliferation of cells, inducing autophagy and fostering inflammatory responses and epithelial mesenchymal transition. By proteomic analysis, they also identified 116 differentially expressed proteins, most of them previously associated with cancer that can be used as biomarkers for lymphoma diagnosis or as potential therapeutic targets [ 209 ].…”

Section: Transcriptomicsmentioning

confidence: 99%

Recent Advances in the Genetic of MALT Lymphomas

Rodríguez‐Sevilla

Salar

2021

Cancers

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Mucosa-associated lymphoid tissue (MALT) lymphomas are a diverse group of lymphoid neoplasms with B-cell origin, occurring in adult patients and usually having an indolent clinical behavior. These lymphomas may arise in different anatomic locations, sharing many clinicopathological characteristics, but also having substantial variances in the aetiology and genetic alterations. Chromosomal translocations are recurrent in MALT lymphomas with different prevalence among different sites, being the 4 most common: t(11;18)(q21;q21), t(1;14)(p22;q32), t(14;18)(q32;q21), and t(3;14)(p14.1;q32). Several chromosomal numerical abnormalities have also been described, but probably represent secondary genetic events. The mutational landscape of MALT lymphomas is wide, and the most frequent mutations are: TNFAIP3, CREBBP, KMT2C, TET2, SPEN, KMT2D, LRP1B, PRDM1, EP300, TNFRSF14, NOTCH1/NOTCH2, and B2M, but many other genes may be involved. Similar to chromosomal translocations, certain mutations are enriched in specific lymphoma types. In the same line, variation in immunoglobulin gene usage is recognized among MALT lymphoma of different anatomic locations. In the last decade, several studies have analyzed the role of microRNA, transcriptomics and epigenetic alterations, further improving our knowledge about the pathogenic mechanisms in MALT lymphoma development. All these advances open the possibility of targeted directed treatment and push forward the concept of precision medicine in MALT lymphomas.

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Proteomic and transcriptomic studies of BGC823 cells stimulated with Helicobacter pylori isolates from gastric MALT lymphoma

Abstract: Background The correlation between the infection of H. pylori and the occurrence of gastric MALT lymphoma (GML) has been well documented. However, the mechanism of how GML is caused by this bacterium is not well understood, although some immunologic mechanisms are thought to be involved.

Cited by 5 publications

References 51 publications

Glycopeptidomics Analysis of a Cell Line Model Revealing Pathogenesis and Potential Marker Molecules for the Early Diagnosis of Gastric MALT Lymphoma

Glycopeptidomics Analysis of a Cell Line Model Revealing Pathogenesis and Potential Marker Molecules for the Early Diagnosis of Gastric MALT Lymphoma

Biofilm and Cancer: Interactions and Future Directions for Cancer Therapy

Recent Advances in the Genetic of MALT Lymphomas

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