Proteomic and transcriptomic approaches to identify resistance and susceptibility related proteins in contrasting rice genotypes infected with fungal pathogen Rhizoctonia solani

Abstract

The devastating sheath blight disease caused by Rhizoctonia solani Kuhn (teleomorph: Thanatephorus cucumeris) causes major yield loss in most rice growing regions of the world. In this study, two moderately tolerant and four susceptible genotypes of rice were selected for R. solani induced proteome analysis using two-dimensional polyacrylamide gel electrophoresis. Forty five differentially expressed proteins (DEPs) were identified and analyzed by Mass Spectrometry. Based on their functions, these proteins were classified into different groups, viz., photosynthesis, resistance and pathogenesis, stress, cell wall metabolism and cytoskeleton development associated proteins, and hypothetical or uncharacterized proteins. Expression of 14 genes encoding DEPs was analyzed by quantitative PCR which showed consistency in transcripts and genes expression pattern. Furthermore, the expression of 16 other genes involved in diverse biological functions was analyzed. Up-regulation of these genes in the tolerant genotype Pankaj during sheath blight disease suggested efficient genetic regulation of this cultivar under stress. Also, expression analysis of conserved microRNAs (miRNAs) and their target genes revealed important role of miRNAs in post-transcriptional gene regulation during development of rice sheath blight disease. Genome-wide discovery of miRNAs and further characterization of DEPs and genes will help in better understanding of the molecular events during sheath blight disease development in rice.

Pectin induced transcriptome of a Rhizoctonia solani strain causing sheath blight disease in rice reveals insights on key genes and RNAi machinery for development of pathogen derived resistance

“…5a). To further confirm the results of detached cut leaf assay, the plants were inoculated by placing the young sclerotium of the R. solani Wgl-2 beneath the rice leaf sheath as described in our earlier study (Prathi et al 2018). The symptoms were recorded at 48, 72 and 96 h after inoculation (Fig.…”

“…In order to analyze the expression of R. solani genes during host-pathogen interaction, six highly diverse rice genotypes were selected i.e., TN1, BPT 5204, Pankaj, Tetep, N22, and Vandana. Among these, Tetep and Pankaj are tolerant while TN1, BPT 5204, N22, and Vandana are susceptible rice cultivars (Prathi et al 2018). Freshly grown (4 days old) uniform sized sclerotia of the pathogen were used for inoculation of rice cultivars (at 45 days old stage) by placing them in sheath and leaf tissues.…”

“…Three replicates with at least three plants of each line in one replicate were evaluated in separate assays. The disease tolerance assay of PG-RNAi plants was also done by detached leaf (Dath 1987) as described in our previous report (Prathi et al 2018).…”

“…All experiments were performed with three biological replicates and three technical replicates. The relative expression of the gene was calculated by 2 −ΔCt method, as described in our earlier study (Prathi et al 2018).…”

Chromosome doubling mediates superior drought tolerance in Lycium ruthenicum via abscisic acid signaling

“…High-throughput omics techniques are widely used to study the interactions of plants with other factors 13 . Highthroughput transcriptome sequencing is an important means of understanding phenotype expression and function and is the basis of research on gene function and structure.…”