Proteomic and physiological assessment of stress sensitive and tolerant variety of tomato treated with brassinosteroids and hydrogen peroxide under low-temperature stress

Khan, Tanveer Alam; Yusuf, Mohammad; Ahmad, Aqeel; Bashir, Zoobia; Saeed, Taiba; Fariduddin, Qazi; Hayat, Shamsul; Mock, Hans‐Peter; Wu, Tianbin

doi:10.1016/j.foodchem.2019.03.029

Cited by 76 publications

(59 citation statements)

References 31 publications

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“…An S‐ribonuclease binding protein Q9FJQ8 is well known for ubiquitin‐protein transferase activity. Through its negative regulation of cysteine‐type endopeptidase activity, it metabolizes the nutritional element synthesis in tomato and possesses an antimicrobial potential as well …”

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

“…Digital images of protein gels were captured for their detailed analyses. The stained protein spots were identified and compared using digital software SAMESPOTS (TotalLab Ltd, Newcastle-upon-Tyne, UK) and TOPSPOT (Kroger and Prehm, Berlin, Germany) as described by Khan et al 24 Protein profiles of all treatments from each cultivar were compared with their respective negative control, and profusion index for each protein species was calculated with the formula devised by Bashir et al 25 Profusion behavior of different protein species was plotted in a matrix plot to screen the proteins by 'profusion index'. It provided the proteins most actively responding/playing roles against environmental stresses and hormonal applications.…”

Section: Effect On Protein Profilementioning

confidence: 99%

“…Through its negative regulation of cysteine-type endopeptidase activity, it metabolizes the nutritional element synthesis in tomato and possesses an antimicrobial potential as well. 24 In the case of both cultivars, the elevation of nutritional elements and disease suppression is directly associated with extraction solvent concentration. If we assume the extraction solvent concentration is as a measure of active compound quantity, then it means that the higher the concentration of extraction solvent the more the bioactivity.…”

Section: Effect On Protein Profilementioning

confidence: 99%

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Benzenedicarboxylic acid upregulates O48814 and Q9FJQ8 for improved nutritional contents of tomato and low risk of fungal attack

et al. 2019

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BACKGROUND Tomato is an important food item and a cocktail of phytonutrients. In the current study, metabolites from a non‐pathogenic fungal species Penicillium oxalicum have been exploited to obtain nutritionally augmented tomato fruits from the plants to better withstand against Alternaria alternata infection. RESULTS Initially, bioactivity‐guided assay and chromatographic analyses identified the bioactive metabolites of P. oxalicum [benzenedicarboxylic acid (BDA) and benzimidazole]. Then, ≥3 times elevated quantities of vitamins and other nutritional elements (protein, fat, fibers, and carbohydrates) were achieved by the foliar application of BDA. The maximum increase (625.81%) was recorded in riboflavin contents; however, thiamine showed the second highest enhancement (542.86%). Plant metabolites analysis revealed that jasmonic acid contents were boosted 121.53% to significantly enhance guaiacyl lignin defenses along with the reduction in coumarin contents. The protein profile analysis explored three most actively responding protein species toward BDA applications, (i) palmitoyltransferase protein Q9FLM3; (ii) serine/threonine‐protein kinase O48814; and (iii) E3 ubiquitin‐protein ligase Q9FJQ8. The O48814 improved plant defenses; whereas, Q9FJQ8 protein was negatively regulating cysteine‐type endopeptidase activity and assisted plant to resist schedule alterations. Tomato cultivar with more active innate metabolism was found to be more responsive toward BDA. Furthermore, the bioactive compounds were enriched by using the two‐step extraction method of ethyl acetate and chloroform, respectively. CONCLUSION Penicillium oxalicum a non‐pathogenic fungal species, produced BDA, induced nutritional contents in tomato and protected it against Alternaria alternata. The current study is the first report on the bioactivity of BDA and benzimidazole concerning the nutritional enhancement and plant defense improvement. © 2019 Society of Chemical Industry

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Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Effect On Protein Profilementioning

confidence: 99%

Section: Effect On Protein Profilementioning

confidence: 99%

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Benzenedicarboxylic acid upregulates O48814 and Q9FJQ8 for improved nutritional contents of tomato and low risk of fungal attack

et al. 2019

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“…Proteins related to temperature-related stress has been extensively in tomato, for example, 67 differentially expressed proteins were identified in tomato seedlings in response to high temperature using 2D-gel electrophoresis and MALDI-TOF/TOF MS ( [83]. In a recent study, proteomic analysis was used to dissect the changes in contrasting tomato varieties under low-temperature stress [84]. Although a number of proteomics analyses have been performed to study abiotic stress response in tomato, data analysis and interpretation is still a bottleneck on the road of in-depth proteomics studies in crops.…”

Section: Tomato Proteomics: Applicability and Challengesmentioning

confidence: 99%

Advances in Omics Approaches for Abiotic Stress Tolerance in Tomato

Chaudhary

Khatri

Singla

et al. 2019

Biology

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Tomato, one of the most important crops worldwide, has a high demand in the fresh fruit market and processed food industries. Despite having considerably high productivity, continuous supply as per the market demand is hard to achieve, mostly because of periodic losses occurring due to biotic as well as abiotic stresses. Although tomato is a temperate crop, it is grown in almost all the climatic zones because of widespread demand, which makes it challenge to adapt in diverse conditions. Development of tomato cultivars with enhanced abiotic stress tolerance is one of the most sustainable approaches for its successful production. In this regard, efforts are being made to understand the stress tolerance mechanism, gene discovery, and interaction of genetic and environmental factors. Several omics approaches, tools, and resources have already been developed for tomato growing. Modern sequencing technologies have greatly accelerated genomics and transcriptomics studies in tomato. These advancements facilitate Quantitative trait loci (QTL) mapping, genome-wide association studies (GWAS), and genomic selection (GS). However, limited efforts have been made in other omics branches like proteomics, metabolomics, and ionomics. Extensive cataloging of omics resources made here has highlighted the need for integration of omics approaches for efficient utilization of resources and a better understanding of the molecular mechanism. The information provided here will be helpful to understand the plant responses and the genetic regulatory networks involved in abiotic stress tolerance and efficient utilization of omics resources for tomato crop improvement.

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The Omics Strategies for Abiotic Stress Responses and Microbe-Mediated Mitigation in Plants

Maitra

Bhadra

Yadav

et al. 2021

Sustainable Development and Biodiversity

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Proteomic and physiological assessment of stress sensitive and tolerant variety of tomato treated with brassinosteroids and hydrogen peroxide under low-temperature stress

Cited by 76 publications

References 31 publications

Benzenedicarboxylic acid upregulates O48814 and Q9FJQ8 for improved nutritional contents of tomato and low risk of fungal attack

Benzenedicarboxylic acid upregulates O48814 and Q9FJQ8 for improved nutritional contents of tomato and low risk of fungal attack

Advances in Omics Approaches for Abiotic Stress Tolerance in Tomato

The Omics Strategies for Abiotic Stress Responses and Microbe-Mediated Mitigation in Plants

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