Proteomic and physiological analysis provides an elucidation of Fusarium proliferatum infection causing crown rot on banana fruit

Xie, Lihong; Wu, Yanrong; Duan, Xuewu; Li, Taotao; Jiang, Yueming

doi:10.1016/j.micres.2021.126952

Cited by 8 publications

(3 citation statements)

References 64 publications

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“…pathogens by up-regulation of some defense genes such as CHIT, GLU and PAL in plants (Karczyński et al, 2023). Plants have a wide range of defense mechanisms, such as morpho-physiology, biochemistry, molecular and hormone signaling, and gene and enzyme activity changes, to resist disease infestation (Fernandes and Ghag, 2022;Xie et al, 2022). Our results convincingly establish S. plymuthica HK9-3 as an inducer of systemic resistance for effective control of P. capsici infection of tomato and a plant growth-promoting agent.…”

Section: Discussionsupporting

confidence: 54%

Serratia plymuthicaHK9‐3 enhances tomato resistance against Phytophthora capsici by modulating antioxidant defense systems and rhizosphere micro‐ecological condition

Wang,

Piao,

et al. 2024

Physiologia Plantarum

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Tomatoes are frequently challenged by various pathogens, among which Phytophthora capsici (P. capsici) is a destructive soil‐borne pathogen that seriously threatens the safe production of tomatoes. Plant growth‐promoting rhizobacteria (PGPR) positively induced plant resistance against multiple pathogens. However, little is known about the role and regulatory mechanism of PGPR in tomato resistance to P. capsici. Here, we identified a new strain Serratia plymuthica (S. plymuthica), HK9‐3, which has a significant antibacterial effect on P. capsici infection. Meanwhile, stable colonization in roots by HK9‐3, even under P. capsici infection, improved tomato growth parameters, root system architecture, photosynthetic capacity, and boosted biomass. Importantly, HK9‐3 colonization significantly alleviated the damage caused by P. capsici infection through enhancing ROS scavenger ability and inducing antioxidant defense system and pathogenesis‐related (PR) proteins in leaves, as evidenced by elevating the activities of peroxidase (POD), superoxide dismutase (SOD), catalase (CAT), ascorbate peroxidase (APX), phenylalanine ammonia lyase (PAL), polyphenol oxidase (PPO), and chitinase, β‐1,3‐glucanase, and increasing the transcripts of POD, SOD, CAT, APX1, PAL1, PAL2, PAL5, PPO2, CHI17 and β‐1,3‐glucanase genes. Notably, HK9‐3 colonization not only effectively improved soil microecology and soil fertility, but also significantly enhanced fruit yield by 44.6% and improved quality. Our study presents HK9‐3 as a promising and effective solution for controlling P. capsici infection in tomato cultivation while simultaneously promoting plant growth and increasing yield, which may have implications for P. capsici control in vegetable production.

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Section: Discussionsupporting

confidence: 54%

Serratia plymuthicaHK9‐3 enhances tomato resistance against Phytophthora capsici by modulating antioxidant defense systems and rhizosphere micro‐ecological condition

Wang,

Piao,

et al. 2024

Physiologia Plantarum

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“…Thioredoxin H1 has been reported to be involved in the plant response to biotic stress (Jiang et al., 2022). Additionally, we found that thioredoxin H1 was down‐regulated by F. proliferatum infection, which might contribute to the decrease in the resistance response of banana fruit (Xie et al., 2022). Leucine aminopeptidase is another protein that has been reported to be involved with plant response to pathogen infection.…”

Section: Discussionmentioning

confidence: 94%

“…Host–pathogen interactions have profound implications in understanding the fungal pathogenesis and enhancing plant resistance. In our prior study, we investigated the infection mechanism of F. proliferatum based on banana host change in response to pathogen infection (Xie et al., 2022). Although the elucidation of the prominent pathways disturbed by F. proliferatum in banana fruit was provided, how F. proliferatum modulates these pathways is largely unknown.…”

Section: Discussionmentioning

confidence: 99%

Possible mechanism of contribution of a secreted aspartic proteinase FpOPSB to the virulence of Fusarium proliferatum causing banana crown rot

Yang

Xie

et al. 2023

Food Frontiers

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Fusarium proliferatum is one of the pathogenic fungi causing crown rot that results in quality deterioration and commercial value loss of banana fruit during postharvest storage. Secreted proteins from pathogenic fungi can be delivered into hosts to suppress plant immunity and establish infection. We reported here a secreted aspartic proteinase OPSB (FpOPSB) that was active during F. proliferatum infection on banana peel. FpOPSB exhibited protease activity based on in vitro assay. Immunohistochemistry assay along with subcellular localization analysis demonstrated that FpOPSB can be secreted into banana peel and localized in cytoplasm. FpOPSB deletion mutant (Δopsb) showed reduced virulence on banana fruit but no obvious effect on fungal growth compared with wild‐type. Subsequently, scanning electron microscopy results suggested that FpOPSB was important for conidial production and infectious growth as well as spreading of F. proliferatum in banana peel. Heterologous expression of FpOPSB in tobacco significantly enhanced plant susceptibility to F. proliferatum. Further, seven proteins were identified as potential targets of FpOPSB in banana peel using immunoprecipitation–mass spectrometry (MS)/MS, including pathogenesis‐related 10, thioredoxin H1, nonsymbiotic hemoglobin 2, fructokinase‐1, patellin‐1, leucine aminopeptidase 2, and l‐lactate dehydrogenase A. Taken together, our results confirmed that the secreted FpOPSB is a critical virulence factor of F. proliferatum when infecting banana fruit. Additionally, we postulated that FpOPSB might interfere the host immune system or neutralize the host defenses, thereby contributing to the pathogenicity of F. proliferatum. We propose aspartic proteinase as a new potential target for controlling fungal disease of postharvest fruit.

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Taxonomic and phylogenetic assessment of selected fungal pathogens associated with banana fruits in the local markets of northern Thailand

Samarakoon,

Wanasinghe

et al. 2024

Eur J Plant Pathol

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Proteomic and physiological analysis provides an elucidation of Fusarium proliferatum infection causing crown rot on banana fruit

Cited by 8 publications

References 64 publications

Serratia plymuthicaHK9‐3 enhances tomato resistance against Phytophthora capsici by modulating antioxidant defense systems and rhizosphere micro‐ecological condition

Serratia plymuthicaHK9‐3 enhances tomato resistance against Phytophthora capsici by modulating antioxidant defense systems and rhizosphere micro‐ecological condition

Possible mechanism of contribution of a secreted aspartic proteinase FpOPSB to the virulence of Fusarium proliferatum causing banana crown rot

Taxonomic and phylogenetic assessment of selected fungal pathogens associated with banana fruits in the local markets of northern Thailand

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