Proteomic and Physiological Analysis of the Response of Oat (Avena sativa) Seeds to Heat Stress under Different Moisture Conditions

Abstract

Seeds lose their viability when they are exposed to high temperature and moisture content (MC) during storage. The expression and metabolism of proteins plays a critical role in seed resistance to heat stress. However, the proteome response to heat stress in oat (Avena sativa) seeds during storage has not been revealed. To understand mechanisms of heat stress acclimation and tolerance in oat seeds, an integrated physiological and comparative proteomic analysis was performed on oat seeds with different MC during heat stress. Oat seeds with 10% and 16% MC were subjected to high temperatures (35, 45, and 50°C) for 24 and 2 days, respectively, and changes in physiological and biochemical characteristics were analyzed. The results showed that seed vigor decreased significantly with temperature increase from 35 to 50°C. Also, the proline content in 10% MC seeds decreased significantly (p < 0.05) whereas that in 16% MC seeds increased significantly (p < 0.05) during heat treatment from 35 to 50°C. There were no significant differences in malondialdehyde content in 10% MC seeds with temperature from 35 to 50°C, but a significant (p < 0.05) decline occurred in 16% MC seeds at 45°C. Proteome analysis revealed 21 significantly different proteins, including 19 down-regulated and two up-regulated proteins. The down-regulated proteins, notably six heat shock proteins and two ATP synthases, have important roles in the mobilization of carbohydrates and energy, and in the balance between synthesis and degradation of other proteins during seed deterioration. The up-regulation of argininosuccinate synthase participated in proline biosynthesis at 16% MC, which is important for maintaining reactive oxygen species homeostasis for the resistance of heat stress. In summary, heat-responsive protein species and mitochondrial respiratory metabolism were sensitive to high temperature and MC treatment. These studies provide a new insight into acclimation and tolerance to heat stress in oat seeds.

Melatonin Priming Alleviates Aging-Induced Germination Inhibition by Regulating β-oxidation, Protein Translation, and Antioxidant Metabolism in Oat (Avena sativa L.) Seeds

“…Under adverse conditions, cells can control proline content by increasing proline synthase expression or inhibiting proline degradation. Chen et al [50] found that the proline content in aged oat seeds decreased significantly and, consequently, seed vigor decreased. Furthermore, δ-1-pyrroline-5-carboxylate synthetase (P5CS) catalyzed the first step of proline synthesis, and the overexpression of P5CS1 improved drought tolerance in rice [51].…”

Cold and Heat Stress Diversely Alter Both Cauliflower Respiration and Distinct Mitochondrial Proteins Including OXPHOS Components and Matrix Enzymes

“…We also found that heat caused a vast increase in abundance of ATP synthase d-subunit, contrary to its major downregulation reported by Gammulla et al [ 37 ] and Tan et al [ 39 ] for cold-stressed Arabidopsis cell cultures and heat-treated rice leaves, respectively. During oat ( Avena sativa ) seed storage, ATP1 level consistently declined as temperature increased from 35 to 50 °C, whereas subunit d of ATP synthase initially increased and then decreased in abundance under the same treatment; notably, subunits d and α were differentially accumulated at 10% and 16% moisture content, respectively [ 102 ]. Overall, those findings suggest that demand for ATP synthesis during heat treatment increases and the excess of de novo synthesized diverse ATP synthase subunits (e.g., mitochondrially encoded ATP1 or nuclear-encoded ATP7 proteins) is likely to be assembled into novel ATP synthase holocomplexes, labile in heat recovery [ 41 ].…”

Quantitative proteomic, physiological and biochemical analysis of cotyledon, embryo, leaf and pod reveals the effects of high temperature and humidity stress on seed vigor formation in soybean

“…A series of germination tests were performed at 24, 96 and 168 h after HTH stress, respectively, according to [50]. Three replications (50 seeds for each treatment) were distributed in plastic boxes (20 × 11.5 × 8 cm) containing six sheets of moistened filter paper.…”