Proteomic and N-glycoproteomic analyses of total subchondral bone protein in patients with primary knee osteoarthritis

Feng, Gangning; Zhou, Yong; Yan, Jiangbo; Wang, Zheng; Yang, Yong; Zhang, Weidong; Wang, Na; Lü, Zan; Chen, Yaogeng; Jin, Qunhua

doi:10.1016/j.jprot.2023.104896

Cited by 2 publications

(1 citation statement)

References 55 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Similarly, the amoebiasis pathway showed overlap with our findings. A recent proteomic analysis of total bone protein in primary knee osteoarthritis patients identified proteins with differential N-glycosylation sites enriched in metabolic pathways such as amoebiasis [40].…”

Section: Discussionmentioning

confidence: 99%

Whole-Transcriptome Sequencing of Knee Joint Cartilage from Kashin–Beck Disease and Osteoarthritis Patients

Han,

Cheng,

Wei

et al. 2024

IJMS

View full text Add to dashboard Cite

The aim of this study was to provide a comprehensive understanding of similarities and differences in mRNAs, lncRNAs, and circRNAs within cartilage for Kashin–Beck disease (KBD) compared to osteoarthritis (OA). We conducted a comparison of the expression profiles of mRNAs, lncRNAs, and circRNAs via whole-transcriptome sequencing in eight KBD and ten OA individuals. To facilitate functional annotation-enriched analysis for differentially expressed (DE) genes, DE lncRNAs, and DE circRNAs, we employed bioinformatic analysis utilizing Gene Ontology (GO) and KEGG. Additionally, using quantitative reverse transcriptase polymerase chain reaction (qRT-PCR), we validated the expression levels of four cartilage-related genes in chondrocytes. We identified a total of 43 DE mRNAs, 1451 DE lncRNAs, and 305 DE circRNAs in KBD cartilage tissue compared to OA (q value < 0.05; |log2FC| > 1). We also performed competing endogenous RNA network analysis, which identified a total of 65 lncRNA-mRNA interactions and 4714 miRNA-circRNA interactions. In particular, we observed that circRNA12218 had binding sites for three miRNAs targeting ACAN, while circRNA12487 had binding sites for seven miRNAs targeting COL2A1. Our results add a novel set of genes and non-coding RNAs that could potentially serve as candidate diagnostic biomarkers or therapeutic targets for KBD patients.

show abstract

Section: Discussionmentioning

confidence: 99%

Whole-Transcriptome Sequencing of Knee Joint Cartilage from Kashin–Beck Disease and Osteoarthritis Patients

Han,

Cheng,

Wei

et al. 2024

IJMS

View full text Add to dashboard Cite

show abstract

The Genetics and Functional Genomics of Osteoarthritis

Arruda,

Katsoula,

Chen

et al. 2024

Annual Review of Genomics and Human Genetics

View full text Add to dashboard Cite

Osteoarthritis is the most prevalent whole-joint degenerative disorder, and is characterized by the degradation of articular cartilage and the underlying bone structures. Almost 600 million people are affected by osteoarthritis worldwide. No curative treatments are available, and management strategies focus mostly on pain relief. Here, we provide a comprehensive overview of the available human genetic and functional genomics studies for osteoarthritis to date and delineate how these studies have helped shed light on disease etiopathology. We highlight genetic discoveries from genome-wide association studies and provide a detailed overview of molecular-level investigations in osteoarthritis tissues, including methylation-, transcriptomics-, and proteomics-level analyses. We review how functional genomics data from different molecular levels have helped to prioritize effector genes that can be used as drug targets or drug-repurposing opportunities. Finally, we discuss future directions with the potential to drive a step change in osteoarthritis research.

show abstract

Proteomic and N-glycoproteomic analyses of total subchondral bone protein in patients with primary knee osteoarthritis

Cited by 2 publications

References 55 publications

Whole-Transcriptome Sequencing of Knee Joint Cartilage from Kashin–Beck Disease and Osteoarthritis Patients

Whole-Transcriptome Sequencing of Knee Joint Cartilage from Kashin–Beck Disease and Osteoarthritis Patients

The Genetics and Functional Genomics of Osteoarthritis

Contact Info

Product

Resources

About