Proteomic and Metabolomic Fingerprinting in Male Infertility

Selvam, Manesh Kumar Panner; Durairajanayagam, Damayanthi; Agarwal, Ashok

doi:10.1007/978-3-030-32300-4_9

Cited by 2 publications

(1 citation statement)

References 108 publications

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“…Male infertility of unknown origin is a condition in which fertility impairment occurs spontaneously or with idiopathic cause [ 3 , 4 , 29 ]. In these situations, proteomic and metabolomic techniques can be applied to explore potential biomarkers for the prognosis of male infertility [ 30 ]. Several works based in proteomic studies indicate that numerous sperm proteins related to motility, metabolism, capacitation, hyperactivation, acrosome reaction [ 31 – 38 ], and those such involved in the recognition or the interaction with the egg and/or the surrounding cells during fertilization may also act as candidates of male fertility biomarkers [ 32 , 39 – 41 ].…”

Section: Discussionmentioning

confidence: 99%

Evaluation of sperm integrin α5β1 as a potential marker of fertility in humans

et al. 2022

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Sperm selection for assisted reproduction techniques is generally based on basic parameters, while key aspects of sperm competence and its journey from the deposition site to the fertilization site are overlooked. Consequently, identifying molecular markers in spermatozoa that can efficiently predict the fertility of a semen sample could be of great interest, particularly in cases of idiopathic male infertility. When spermatozoa reach the female reproductive tract, it provides to them the cellular and molecular microenvironment needed to acquire fertilizing ability. In this sense, considering the role that integrin α5β1 of spermatozoa plays in reproduction-related events, we investigated the correlation between the subcellular localization of sperm integrin α5β1 and early embryo development outcome after in vitro fertilization (IVF) procedures in human. Twenty-four semen samples from normozoospermic men and metaphase II (MII) oocytes from healthy women aged under 38 years, from couples who underwent IVF cycles, were used in this work. Sperm α5β1 localization was evaluated by immunofluorescence assay using an antibody against integrin α5 subunit. Integrin α5β1 was mainly localized in the sperm acrosomal region (45.33±7.89%) or the equatorial segment (30.12±7.43%). The early embryo development rate (data obtained from the Fertility Center) correlated positively with the localization of α5β1 in the acrosomal region (number of usable embryos / inseminated oocytes: ρ = 0.75; p<0.01 and number of usable embryos/total number of two pronuclear zygotes: ρ = 0.80; p<0.01). However, this correlation was not significant when the equatorial segment mark was evaluated. In addition, human sperm released from co-culture with bovine oviductal epithelial cells (BOEC) showed a significant enrichment in the acrosomal localization pattern of α5β1 compared to those sperm that were not co-cultured with BOEC (85.20±5.35% vs 35.00±17.09%, respectively, p<0.05). In conclusion, the evaluation of sperm integrin α5β1 immunolocalization could be a useful tool to select sperm with fertilizing ability from human semen samples before IVF procedures.

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Section: Discussionmentioning

confidence: 99%

Evaluation of sperm integrin α5β1 as a potential marker of fertility in humans

et al. 2022

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Viral Effect of Sars-Cov-2 on Male Infertility

Sapozhkova¹

2021

Laboratornaya I Klinicheskaya Meditsina. Farmatsiya

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Clinical laboratory diagnostics of male infertility is an integral part of the diagnostic process in the field of reproductive medicine. Studies conducted during the pandemia of novel coronavirus infection (COVID-19) indicate that RNA of SARS-CoV-2 can be detected in the testes, and that the possibility of disrupting spermatogenesis and the viral impact on male reproductive health has been predicted.

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Proteomic and Metabolomic Fingerprinting in Male Infertility

Cited by 2 publications

References 108 publications

Evaluation of sperm integrin α5β1 as a potential marker of fertility in humans

Evaluation of sperm integrin α5β1 as a potential marker of fertility in humans

Viral Effect of Sars-Cov-2 on Male Infertility

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