Proteomic and Metabolomic Analyses of the Inflorescences Axis of Castor (Ricinus conununis L.) at Different Developmental Stages

Abstract

Background: Castor is a dicotyledon, ricinus communis, Euphorbiaceae, and is oneof world ten oil crops. Lm female line castor has three inflorescence types(female with marker, haplometrotic and monoecious). Female with marker castor was realized as a restorer line and as a maintainer line,which was applied to castor hybrid breeding. The developmental mechanism of the three inflorescences is not clear.Results: Therefor, in this study , proteomic and metabolomic analyses of different inflorescences axis were performed. Seventy-three diferentially abundant protein species (DAPS) were detected in the proteomics analysis and 86 differentially abundant metabolites (DAMs) were identified from the proteomic and metabolomic analyses of the inflorescences axis, respectively. These DAPS and DAMs are primarily mainly involved in carbon metabolism, comprising carbon fixation in the photosynthetic organism pathway, the glycolysis pathway, carbon metabolic pathways, and the biosynthesis of amino acids. Quantitative real-time PCR (qRT-PCR) results demonstrated that the proteomics and metabolomic data collected in this study were reliable.Conclusions: The results of this study provides some valuable insights into the inflorescence development of castor: Specifically, the results showed that DAPS and DAMs are involved in photosynthesis and respiration to control the distribution of imported carbohydrates and exported photoassimilates and thus affect inflorescence development of castor. Furthermore, the dynamic balance of amino acids in different types of inflorescences was also shown to be essential in affecting inflorescence structure.