Proteomic and histopathological response in the gills of Poecilia reticulata exposed to glyphosate-based herbicide

Rocha, Thiago Lopes; Santos, Alex Caetano dos; Yamada, Áureo T.; Soares, Célia Maria de Almeida; Borges, Clayton Luiz; Bailão, Alexandre Melo; Sabóia‐Morais, Simone Maria Teixeira de

doi:10.1016/j.etap.2015.04.016

Cited by 42 publications

(19 citation statements)

References 34 publications

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“…These results observed in both genders are consistent with the previous studies and support the fact that the GBH in different forms (e.g. active ingredient, sub‐product of degradation, surfactant and commercial formulation) induce several histopathological changes in the fish gills (Hued et al ., ; Jiraungkoorskul et al ., ; Rocha et al ., ), which can lead to hypoxia and death.…”

Section: Discussionmentioning

confidence: 99%

“…The genotoxic and cytotoxic effects of the GBH in fish species (e.g. P. reticulata , Prochilodus lineatus and Colossoma macropomum ) were also reported previously, indicating their ability to induce the generation of reactive oxygen species (directly and indirectly), oxidative stress, and genotoxic and tissue damages (Braz‐Mota et al ., ; Ghisi et al ., ; Moreno et al ., ; Rocha et al ., ; Souza Filho et al ., ).…”

Section: Discussionmentioning

confidence: 99%

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Gender‐specific histopathological response in guppies Poecilia reticulata exposed to glyphosate or its metabolite aminomethylphosphonic acid

Antunes

Rocha

Pires

et al. 2017

J of Applied Toxicology

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Ecotoxicity of glyphosate (GLY) and its metabolite aminomethylphosphonic acid (AMPA) was investigated in guppies, Poecilia reticulata. We tested the effects of these chemicals on the gills and liver of both male and female guppies using qualitative and quantitative histopathological analyses associated with histopathological condition indexes. Both genders showed similar median lethal concentration (LC ) at 96 h for GLY (68.78 and 70.87 mg l ) and AMPA (180 and 164.32 mg l ). However, the histopathological assessment of both fish organs exposed to sublethal concentrations of GLY (35 mg l ) and AMPA (82 mg l ) for 96 h showed a tissue- and gender-specific histopathological response. In both exposure assays, fish presented mainly progressive changes, such as proliferation of the interlamellar epithelium, partial and total fusion of secondary lamellae. The liver showed mainly regressive changes, such as steatosis, pyknotic nuclei and high distribution of collagen fibers. Unusually large hepatocytes as degenerated cells were also detected. Histopathological changes in gills were similar for the males and females, but the liver response was different between the genders. The hepatic inflammatory changes were more common in males. The increase in the area of hepatocyte vacuoles is gender dependent with higher values in the male compared to the female guppies exposed to GLY and AMPA. Multiparametric analysis indicated that the male guppies are more sensitive than females, particularly in the presence of AMPA. Our study shows that the histopathological assessment associated with gender-specific response can be successfully used in ecotoxicological assessment of GLY and the metabolite AMPA. Copyright © 2017 John Wiley & Sons, Ltd.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Gender‐specific histopathological response in guppies Poecilia reticulata exposed to glyphosate or its metabolite aminomethylphosphonic acid

Antunes

Rocha

Pires

et al. 2017

J of Applied Toxicology

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“…Glyphosate and AMPA were found toxic to guppies Poecilia reticulata (Antunes et al, 2017) with median lethal concentrations (LC 50 ) for 96-h exposure of 0.07 and 0.16-0.18 mg/ml, respectively, with tissue-and gender-specific histopathological responses at sublethal concentrations. Formulated glyphosate preparations (Roundup R , Roundup Transorb R ) exerted even stronger toxicity to this and related guppy species (De Souza Filho et al, 2013;Harayashiki et al, 2013;Rocha et al, 2015;dos Santos et al, 2017) with exposure-time dependent hepatic histopathological damage (dos Santos et al, 2017) or genotoxicity (De Souza Filho et al, 2013). Roundup R was shown to affect hematological response and tissue AChE activity in carp (C. carpio) (GholamiSeyedkolaei et al, 2013;Kondera et al, 2018), increased glycogen and triacylglycerol consumption and lipid deposition in the liver, as well as changes in muscle glycogen in catfish (Rhamdia quelen) (Persch et al, 2018), DNA strand breaks in the goldfish (Carassius auratus) (Çavas and Könen, 2007;Lushchak et al, 2009;Li et al, 2017).…”

Section: Registration Of Glyphosate In the European Unionmentioning

confidence: 99%

Re-registration Challenges of Glyphosate in the European Union

Székacs

Darvas

2018

Front. Environ. Sci.

104

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“…According to Rocha et al (2015), herbicides can induce environmental stress in aquatic organisms, as well as damaging fish respiratory system by reducing oxygen absorption, absorption, which leads to hypoxia.…”

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confidence: 99%

“…It is necessary to carry out studies evaluating different environments and the effect of exposing animals to concentrations of herbicides over time (Rocha et al, 2015).…”

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confidence: 99%

Ecotoxicological evaluation of Glyphosate use in controlling E. crassipes e Salvinia sp. macrophytes

Maria¹,

Castro²,

Lange³

et al. 2024

Ecotoxicol. Environ. Contam.

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The present work aimed to carry out traditional ecotoxicological and ecosystemic tests in microcosms for the evaluation of the use of glyphosate herbicide adopted to control floating aquatic macrophytes in aquatic ecosystems. Ecotoxicity tests were performed using standardized methodology and standard species of different trophic levels. Although the active ingredient glyphosate is toxic, the toxicity of its commercial formulation is higher. The inert ingredient of the Roundup Original ® composition may be responsible for the higher commercial compound toxicity. Experiments in microcosms indicated that the use of the formulation is possible, in considering tested concentration and controlling the spray process, without causing toxic effect on the aquatic ecosystem. Complementary studies evidenced the risk of using the product in aquatic environments without vegetation cover, being not recommended the herbicide application in a place without floating aquatic macrophytes cover.

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Proteomic and histopathological response in the gills of Poecilia reticulata exposed to glyphosate-based herbicide

Cited by 42 publications

References 34 publications

Gender‐specific histopathological response in guppies Poecilia reticulata exposed to glyphosate or its metabolite aminomethylphosphonic acid

Gender‐specific histopathological response in guppies Poecilia reticulata exposed to glyphosate or its metabolite aminomethylphosphonic acid

Re-registration Challenges of Glyphosate in the European Union

Ecotoxicological evaluation of Glyphosate use in controlling E. crassipes e Salvinia sp. macrophytes

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