Proteomic and gene expression differences between post-diapause and subitaneous offspring phenotypes in the cyclic parthenogen Daphnia pulex

Kaupinis, Algirdas; Aitmanaitė, Lina; Strepetkaitė, Dovilė; Valius, Mindaugas; Lazutka, Juozas R.; Arbačiauskas, Кęstutis

doi:10.1007/s10750-016-3057-3

Cited by 4 publications

(1 citation statement)

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“…In fact, an Hsp60 was shown to be upregulated in the post-diapause of Bactrocera minax (Enderlein) (Diptera: Tephritidae) (Dong et al, 2014). Other heat shock proteins, such as the Hsp90 family, were also proposed to have a role in diapause termination and post-diapause development in S. crassipalpis and Lucilia sericata (Meigen) (Diptera, Calliphoridae) Tachibana et al, 2005) as well as in post-diapause offspring of the crustacean Daphnia pulex (Kaupinis et al, 2017). Clearly, more research is required to test the importance of heat shock proteins for diapause egg survival and post-diapause of M. spectabilis.…”

Section: Discussionmentioning

confidence: 99%

Proteins from eggs of the spittlebug Mahanarva spectabilis (Hemiptera: Cercopidae) reveal clues about its diapause regulation

Saraiva

Auad²,

Barros

et al. 2021

Bull. Entomol. Res.

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Embryo development in eggs of the spittlebug Mahanarva spectabilis (Distant) (Hemiptera: Cercopidae) passes through four phases (known as S1 to S4) being stopped at S2 during diapause. Studies about the molecular basis of diapause in spittlebugs are nonexistent. Here, we analyzed proteins from non-diapausing (ND), diapausing (D) and post-diapausing (PD) eggs of the spittlebug M. spectabilis. In total, we identified 87 proteins where 12 were in common among the developmental and diapause phases and 19 remained as uncharacterized. Non-diapausing eggs (S2ND and S4ND) showed more proteins involved in information storage and processing than the diapausing ones (S2D). Eggs in post-diapausing (S4PD) had a higher number of proteins associated with metabolism than S2D. The network of protein interactions and metabolic processes allowed the identification of different sets of molecular interactions for each developmental and diapause phases. Two heat shock proteins (Hsp65 and Hsp70) along with two proteins associated with intracellular signaling (MAP4K and a serine/threonine-protein phosphatase) were found only in diapausing and/or post-diapausing eggs and are interesting targets to be explored in future experiments. These results shine a light on one key biological process for spittlebug survival and represent the first search for proteins linked to diapause in this important group of insects.

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Section: Discussionmentioning

confidence: 99%