Proteomic analysis upon peach fruit infection with Monilinia fructicola and M. laxa identify responses contributing to brown rot resistance

Abstract

Brown rot, caused by Monilinia spp., is a major peach disease worldwide. in this study, the response of peach cultivars Royal Glory (RG) and Rich Lady (RL) to infection by Monilinia fructicola or Monilinia laxa, was characterized. Phenotypic data, after artificial inoculations, revealed that 'RL' was relatively susceptible whereas 'RG' was moderately resistant to Monilinia spp. comparative proteomic analysis identified mesocarp proteins of the 2 cultivars whose accumulation were altered by the 2 Monilinia species. Functional analysis indicated that pathogen-affected proteins in 'RG' were mainly involved in energy and metabolism, while, differentially accumulated proteins by the pathogen presence in 'RL' were involved in disease/defense and metabolism. A higher number of proteins was differentiated in 'RG' fruit compared to 'RL'. Upon Monilinia spp. infection, various proteins were-down accumulated in 'RL' fruit. Protein identification by mass spectrometric analysis revealed that several defense-related proteins including thaumatin, formate dehydrogenase, S-formylglutathione hydrolase, cBS domaincontaining protein, HSP70, and glutathione S-transferase were up-accumulated in 'RG' fruit following inoculation. The expression profile of selected defense-related genes, such as major latex allergen, 1-aminocyclopropane-1-carboxylate deaminase and UDP-glycoltransferase was assessed by RT-PCR. This is the first study deciphering differential regulations of peach fruit proteome upon Monilinia infection elucidating resistance responses.

Shotgun proteomics of peach fruit reveals major metabolic pathways associated to ripening

“…2). Comparison with iTRAQ proteome datasets would be highly informative, however only differentially accumulated proteins are reported in these publications with emphasis on the effect of postharvest treatments such as cold storage or disease incidence in ripening process [35][36][37][38][39] (Supplementary Table 3).…”

Unraveling Interactions of the Necrotrophic Fungal Species Botrytis cinerea With 1-Methylcyclopropene or Ozone-Treated Apple Fruit Using Proteomic Analysis

“…The ripening of fleshy fruit is associated with changes in susceptibility to pathogen infection since susceptibility significantly increases during ripening ( Papavasileiou et al, 2020 ). Apple is a typical climacteric fruit, and its ripening process and postharvest behavior is controlled by ethylene ( Karagiannis et al, 2018 ).…”

“…Proteomic analysis has been used to investigate complex biological processes such as the responses of fruit tissues to pathogens ( Papavasileiou et al, 2020 ). With the use of proteomics, the response of B. cinerea to plant-based elicitors and hormones ( Liñeiro et al, 2016 ) and the in vitro secretome of B. cinerea related to pathogenesis ( González-Fernández et al, 2015 ) have been investigated.…”

Ethylene biosynthesis and response factors are differentially modulated during the interaction of peach petals with Monilinia laxa or Monilinia fructicola

“…The same authors also reported that other pathogen responses such as expression of M. fructicola polygalacturonases took also place as early as 4 hpi. A recently published proteomic study revealed that 1-aminocyclopropane-1-carboxylate deaminase is increased in peach fruits inoculated with M. laxa [51]. Thus, the increased abundance of this enzyme, involved in the conversion of ACC to α-ketobutylate, could, in part, explain the reduced ethylene levels observed in M. laxa inoculated blossoms.…”