Proteomic analysis reveals the temperature-dependent presence of extracytoplasmic peptidases in the biofilm exoproteome of Listeria monocytogenes EGD-e

Lee, Yue-Jia; Wang, Chinling

doi:10.1007/s12275-020-9522-8

Cited by 4 publications

(2 citation statements)

References 55 publications

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“…Numerous cytoplasmic proteins can be detected extracellularly under certain circumstances, e.g., during stress [10,39]. It is possible that some classified as cytoplasmic proteins harbor domains-known or not-that prompt their secretion during bile stress, and thus, they may have extracellular functions.…”

Section: Cytoplasmic Proteins Function As a Bile-stress Protective Mamentioning

confidence: 99%

Exoproteome Perspective on the Bile Stress Response of Lactobacillus johnsonii

Bagon

Valeriano

et al. 2021

Proteomes

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Probiotics must not only exert a health-promoting effect but also be capable of adapting to the harsh environment of the gastrointestinal (GI) tract. Probiotics in the GI tract must survive the cell wall-disrupting effect of bile acids. We investigated the exoproteome of Lactobacillus johnsonii PF01 and C1-10 under bile stress. A comparative analysis revealed the similarities between the two L. johnsonii exoproteomes, as well as their different responses to bile. The large number of metabolic proteins in L. johnsonii revealed its metabolic adaptation to meet protein synthesis requirements under bile stress. In addition, cell wall modifications occurred in response to bile. Furthermore, some extracellular proteins of L. johnsonii may have moonlighting function in the presence of bile. Enolase, L-lactate dehydrogenase, glyceraldehyde-3-phosphate dehydrogenase, triosephosphate isomerase, 50s ribosomal protein L7/L12, and cellobiose-specific phosphotransferase system (PTS) sugar transporter were significantly upregulated under bile stress, suggesting a leading role in the collective bile stress response of L. johnsonii from its exoproteome perspective.

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Section: Cytoplasmic Proteins Function As a Bile-stress Protective Mamentioning

confidence: 99%

Exoproteome Perspective on the Bile Stress Response of Lactobacillus johnsonii

Bagon

Valeriano

et al. 2021

Proteomes

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“…Proteomic analysis of biofilms has been used to identify key proteins that are essential in complex biofilm networks, including; in the EPS ( Egorova et al., 2022 ) , required for temperature adaptation stress ( Lee and Wang, 2020 ) , required for adaptation to antimicrobial treatment ( MaChado and Coquet, 2016 ) , and that change with biofilm development ( Serra et al., 2008 ; Suriyanarayanan et al., 2018 ) and aging ( Rahman et al., 2022 ), and between planktonic and biofilm conditions ( Khan et al., 2019 ; Suryaletha et al., 2019 ; Llama-Palacios et al., 2020 ; Tang et al., 2020 ). These studies use a range of different mass spectrometry methodologies, including tandem liquid chromatography (LC-MS/MS) alone ( Lee and Wang, 2020 ) or coupled with nano-high performance liquid chromatography (HPLC) ( Egorova et al., 2022 ), lab-on-a-chip and XCT mass spectrometry ( MaChado and Coquet, 2016 ), tandem mass tag (TMT) mass spectrometry ( Rahman et al., 2022 ), fourier transform infrared spectroscopy (FT-IR) ( Serra et al., 2008 ), nano-LC/MS coupled with quadrupole-ToF (Q-ToF) ( Suryaletha et al., 2019 ) , iTRAQ labelling plus LC-MS/MS for quantitative proteomics, and matrix-assisted laser desorption ionisation time-of-flight (MALDI-ToF) mass spectrometry ( Llama-Palacios et al., 2020 ; Tang et al., 2020 ). MALDI-ToF analysis of bacteria is routinely used in clinical diagnostic laboratories to identify bacterial species, and this method is increasingly being developed to discriminate between isolates and their status as biofilm-producers or non-producers ( Caputo et al., 2018 ), which will aid in establishing effective antimicrobial therapy.…”

Section: Genetic and Molecular Analysis Techniquesmentioning

confidence: 99%

How to study biofilms: technological advancements in clinical biofilm research

Cleaver,

Garnett

2023

Front. Cell. Infect. Microbiol.

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Biofilm formation is an important survival strategy commonly used by bacteria and fungi, which are embedded in a protective extracellular matrix of organic polymers. They are ubiquitous in nature, including humans and other animals, and they can be surface- and non-surface-associated, making them capable of growing in and on many different parts of the body. Biofilms are also complex, forming polymicrobial communities that are difficult to eradicate due to their unique growth dynamics, and clinical infections associated with biofilms are a huge burden in the healthcare setting, as they are often difficult to diagnose and to treat. Our understanding of biofilm formation and development is a fast-paced and important research focus. This review aims to describe the advancements in clinical biofilm research, including both in vitro and in vivo biofilm models, imaging techniques and techniques to analyse the biological functions of the biofilm.

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