Proteomic analysis reveals that MAEL, a component of nuage, interacts with stress granule proteins in cancer cells

Yuan, Liqin; Xiao, Yuzhong; Zhou, Qiuzhi; Yuan, Dongmei; Wu, Baiping; Chen, Gannong; Zhou, Jianlin

doi:10.3892/or.2013.2836

Cited by 23 publications

(18 citation statements)

References 44 publications

(40 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Although Mael protein is barely detectable in most normal somatic tissues except testis, recent reports have shown that the protein is highly expressed in somatic cancer patient tissues and cancer cell lines [15–18]. Consistent with these reports, RT-PCR and Western blotting demonstrated Mael overexpression in tumor tissues of HCC patients compared with corresponding adjacent liver tissues (Supplementary Figure S2B).…”

Section: Resultssupporting

confidence: 79%

“…Mael is overexpressed various cell lines derived from a majority of cancer types including lung, breast, prostate and colon through epigenetic modifications [15] and is overexpressed in epithelial ovarian cancer tissue [16] and promotes hepatocellular carcinoma metastasis [17]. Proteomic analysis reveals that Mael interacts with stress granule proteins in cancer cells [18]. …”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Mael is essential for cancer cell survival and tumorigenesis through protection of genetic integrity

et al. 2016

View full text Add to dashboard Cite

Germ line-specific genes are activated in somatic cells during tumorigenesis, and are accordingly referred to as cancer germline genes. Such genes that act on piRNA (Piwi-interacting RNA) processing play an important role in the progression of cancer cells. Here, we show that the spermatogenic transposon silencer maelstrom (Mael), a piRNA-processing factor, is required for malignant transformation and survival of cancer cells. A specific Mael isoform was distinctively overexpressed in diverse human cancer cell lines and its depletion resulted in cancer-specific cell death, characterized by apoptosis and senescence, accompanied by an increase in reactive oxygen-species and DNA damage. These biochemical changes and death phenotypes induced by Mael depletion were dependent on ATM. Interestingly Mael was essential for Myc/Ras-induced transformation, and its overexpression inhibited Ras-induced senescence. In addition, Mael repressed retrotransposon activity in cancer cells. These results suggest that Mael depletion induces ATM-dependent DNA damage, consequently leading to cell death specifically in cancer cells. Moreover, Mael possesses oncogenic potential that can protect against genetic instability.

show abstract

Section: Resultssupporting

confidence: 79%

Section: Introductionmentioning

confidence: 99%

Mael is essential for cancer cell survival and tumorigenesis through protection of genetic integrity

et al. 2016

View full text Add to dashboard Cite

show abstract

“…In our previous study, ILKAP was identified to be a component of the MAEL protein complex [ 22 ]. To confirm the protein interaction between MAEL and ILKAP, we performed co-immunoprecipitation (co-IP).…”

Section: Resultsmentioning

confidence: 99%

MAEL contributes to gastric cancer progression by promoting ILKAP degradation

et al. 2017

Self Cite

View full text Add to dashboard Cite

The cancer-testis gene MAEL is involved in the development and progression of bladder, liver and colorectal cancers. However, its role in other cancers is unclear. By systematically analyzing transcriptomics and genomics data from various cancer databases, we identified that the MAEL gene is aberrantly elevated in gastric cancer (GC) tissues and that its expression is strongly negatively correlated with DNA methylation (Pearson's correlation coefficient = −0.675). Survival analysis revealed that MAEL expression may serve as a prognostic marker for GC patients (overall survival: hazard ratio [HR] = 1.54, p = 1.2E-4; first progression: HR = 1.51, p = 8.7E-4). In vitro and in vivo experiments demonstrated that silencing MAEL expression in the GC cell lines HGC-27 and AGS inhibits proliferation, colony formation, migration, invasion and growth of xenograft tumors, whereas MAEL overexpression exerts the opposite effects in the normal gastric cell line GES-1. Mechanistically, MAEL promotes the lysosome-dependent degradation of the protein phosphatase ILKAP, leading to increased phosphorylation of its substrates (p38, CHK1 and RSK2). Moreover, adenovirus-mediated ILKAP overexpression reversed the oncogenic effects of MAEL in vitro and in vivo. Taken together, these results indicate that MAEL exerts its oncogenic function by promoting ILKAP degradation in the GC.

show abstract

“…MAEL is also a cancer testis gene regulated by DNA methylation. It interacts with stress gene in cancer cells and promotes hepatocellular carcinoma metastasis by inducing epithelial-mesenchymal transition 38 . This implies lncRNA AC145110.1 is likely to be a hub of the lncRNA-mRNA regulatory network and may thus be involved in the important process of breast cancer development.…”

Section: Discussionmentioning

confidence: 99%

Tissue-specific Co-expression of Long Non-coding and Coding RNAs Associated with Breast Cancer

Wagner

Hao

et al. 2016

Sci Rep

View full text Add to dashboard Cite

Inference of the biological roles of lncRNAs in breast cancer development remains a challenge. Here, we analyzed RNA-seq data in tumor and normal breast tissue samples from 18 breast cancer patients and 18 healthy controls and constructed a functional lncRNA-mRNA co-expression network. We revealed two distinctive co-expression patterns associated with breast cancer, reflecting different underlying regulatory mechanisms: (1) 516 pairs of lncRNA-mRNAs have differential co-expression pattern, in which the correlation between lncRNA and mRNA expression differs in tumor and normal breast tissue; (2) 291 pairs have dose-response co-expression pattern, in which the correlation is similar, but the expression level of lncRNA or mRNA differs in the two tissue types. We further validated our findings in TCGA dataset and annotated lncRNAs using TANRIC. One novel lncRNA, AC145110.1 on 8p12, was found differentially co-expressed with 127 mRNAs (including TOX4 and MAEL) in tumor and normal breast tissue and also highly correlated with breast cancer clinical outcomes. Functional enrichment and pathway analyses identified distinct biological functions for different patterns of co-expression regulations. Our data suggested that lncRNAs might be involved in breast tumorigenesis through the modulation of gene expression in multiple pathologic pathways.

show abstract

Proteomic analysis reveals that MAEL, a component of nuage, interacts with stress granule proteins in cancer cells

Cited by 23 publications

References 44 publications

Mael is essential for cancer cell survival and tumorigenesis through protection of genetic integrity

Mael is essential for cancer cell survival and tumorigenesis through protection of genetic integrity

MAEL contributes to gastric cancer progression by promoting ILKAP degradation

Tissue-specific Co-expression of Long Non-coding and Coding RNAs Associated with Breast Cancer

Contact Info

Product

Resources

About