Proteomic analysis reveals that auxin homeostasis influences the eighth internode length heterosis in maize (Zea mays)

Chen, Yongqiang; Zhou, Qingqian; Tian, Runmiao; Ma, Zhihui; Zhao, Xiaofeng; Tang, Jihua

doi:10.1038/s41598-018-23874-6

Cited by 20 publications

(16 citation statements)

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“…Transcriptome and other omics have provided powerful strategies for elucidating the molecular basis of heterosis in diverse plants [ 12 , 15 , 20 ]. In the present study, the transcriptome data confirmed that the hybridization activated more genes in the hybrid broccoli with obviously yield heterotic trait than those in their parents.…”

Section: Discussionmentioning

confidence: 99%

Transcriptome and DNA methylome reveal insights into yield heterosis in the curds of broccoli (Brassica oleracea L var. italic)

Yuan

et al. 2018

BMC Plant Biol

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BackgroundCurds are the main edible organs, which exhibit remarkable yield heterosis in F1 hybrid broccoli. However, the molecular basis underlying heterosis in broccoli remains elusive.ResultsIn the present study, transcriptome profiles revealed that the hybridization made most genes show additive expression patterns in hybrid broccoli. The differentially expressed genes including the non-additively expressed genes detected in the hybrid broccoli and its parents were mainly involved in light, hormone and hydrogen peroxide-mediated signaling pathways, responses to stresses, and regulation of floral development, which suggested that these biological processes should play crucial roles in the yield heterosis of broccoli. Among them, light and hydrogen peroxide-mediated signaling pathways represent two novel classes of regulatory processes that could function in yield or biomass heterosis of plants. Totally, 53 candidate genes closely involved in curd yield heterosis were identified. Methylome data indicated that the DNA methylation ratio of the hybrids was higher than that of their parents. However, the DNA methylation levels of most sites also displayed additive expression patterns. These sites with differential methylation levels were predominant in the intergenic regions. In most cases, the changes of DNA methylation levels in gene regions did not significantly affect their expression levels.ConclusionsThe differentially expressed genes, the regulatory processes and the possible roles of DNA methylation modification in the formation of curd yield heterotic trait were discovered. These findings provided comprehensive insights into the curd yield heterosis in broccoli, and were significant for breeding high-yield broccoli varieties.Electronic supplementary materialThe online version of this article (10.1186/s12870-018-1384-4) contains supplementary material, which is available to authorized users.

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Section: Discussionmentioning

confidence: 99%

Transcriptome and DNA methylome reveal insights into yield heterosis in the curds of broccoli (Brassica oleracea L var. italic)

Yuan

et al. 2018

BMC Plant Biol

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“…This similitude seems to be the result of duplication events among the genes and a domain rearrangement due to alternative splicing 63,64 , which has modified the number of ARF genes in different species 53,62 . An expression analysis in silico of ZmARF27 showed that this gene in maize is mainly expressed in the seed, tassel and ear 59 , tissues affected by auxin concentration [65][66][67] . For instance, Chen et al 65 found that there is a positive correlation between IAA concentration and ear height.…”

Section: Discussionmentioning

confidence: 99%

“…An expression analysis in silico of ZmARF27 showed that this gene in maize is mainly expressed in the seed, tassel and ear 59 , tissues affected by auxin concentration [65][66][67] . For instance, Chen et al 65 found that there is a positive correlation between IAA concentration and ear height. On the other hand, Erkoyuncu et al 67 reported that high concentrations of 2,4-D increase callus formation in maize embryos.…”

Section: Discussionmentioning

confidence: 99%

Auxin perception in Agave is dependent on the species’ Auxin Response Factors

Cancino-García

Ramírez-Prado

De‐la‐Peña

2020

Sci Rep

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Auxins are one of the most important and studied phytohormones in nature. Auxin signaling and perception take place in the cytosol, where the auxin is sensed. then, in the nucleus, the auxin response factors (ARf) promote the expression of early-response genes. it is well known that not all plants respond to the same amount and type of auxins and that the response can be very different even among plants of the same species, as we present here. Here we investigate the behavior of ARf in response to various auxins in Agave angustifolia Haw., A. fourcroydes Lem. and A. tequilana Weber var. Azul. By screening the available database of A. tequilana genes, we have identified 32 ARF genes with high sequence identity in the conserved domains, grouped into three main clades. A phylogenetic tree was inferred from alignments of the 32 Agave ARF protein sequences and the evolutionary relationship with other species was analyzed. AteqARF 4, 15, 21, and 29 were selected as a representative diverse sample coming from each of the different subclades that comprise the two main clades of the inferred phylogenetic reconstruction. these ARFs showed differential species-specific expression patterns in the presence of indole-3-acetic acid (IAA) and 2,4-dichlorophenoxyacetic acid (2,4-D). Interestingly, A. angustifolia showed different phenotypes in the presence and absence of auxins. In the absence of auxin, A. angustifolia produces roots, while shoots are developed in the presence of iAA. However, in the presence of 2,4-D, the plant meristem converts into callus. According to our results, it is likely that AteqARF15 participates in this outcome.

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“…Thus, the decrease in abundance of indole-3-acetaldehyde oxidase protein could have influenced IAA biosynthesis, which is important for rapid seed germination in F 1 hybrids. Recently, Chen et al (2018) using proteomic analysis revealed the importance of auxin homeostasis on eighth internode length heterosis in maize.…”

Section: Deps Are Associated With Different Cellular Processesmentioning

confidence: 99%

Proteomic analysis of heterotic seed germination in maize using F1 hybrid DHM 117 and its parental inbreds

Meena¹,

Pullaiahgari²,

Gudipalli³

2018

Turk J Biol

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A number of differentially expressed proteins (DEPs) were identified in comparative two-dimensional gel electrophoresis analysis of dry and 24-h water-imbibed seeds of maize F 1 hybrid DHM 117 (BML 6 × BML 7) and its parental inbreds. Of the DEPs, 53.4% (86/161) in dry seeds and 58% (127/219) in water-imbibed seeds exhibited a nonadditive pattern in the F 1 hybrid as compared to parental inbreds. A total of 30 DEPs were categorized into different biological processes, most of which were related to metabolism and energy (34%), followed by storage proteins (27%), stress response (23%), transcription and translation (7%), cell cycle (3%), and hormone biosynthesis (3%). The transcript accumulation pattern of 8 selected genes corresponding to DEPs was examined using qRT-PCR. Interestingly, LEA protein Rab28 showed higher accumulation in dry seeds at both protein and transcript levels, whereas indole-3-acetaldehyde oxidase showed lower accumulation in water-imbibed seeds of the F 1 hybrid than the female parent at the protein level. Thus, the DEPs particularly involved in metabolic and energy processes, as well as hormone biosynthesis in the F 1 hybrid, might be responsible for heterotic seed germination in the F 1 hybrid. The DEPs identified in this study provide a scope for improving the seed germination trait of agricultural crops.

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Proteomic analysis reveals that auxin homeostasis influences the eighth internode length heterosis in maize (Zea mays)

Cited by 20 publications

References 54 publications

Transcriptome and DNA methylome reveal insights into yield heterosis in the curds of broccoli (Brassica oleracea L var. italic)

Transcriptome and DNA methylome reveal insights into yield heterosis in the curds of broccoli (Brassica oleracea L var. italic)

Auxin perception in Agave is dependent on the species’ Auxin Response Factors

Proteomic analysis of heterotic seed germination in maize using F1 hybrid DHM 117 and its parental inbreds

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