Proteomic analysis reveals that a global response is induced by subinhibitory concentrations of ampicillin

Abstract

In this study, a recipient-donor co-culture system was used to research the effect of subinhibitory concentrations of antibiotics on horizontal transmission in bacteria and the influence of antibiotics on protein expression. We employed two-dimensional gel electrophoresis combined with mass spectrometry to compare the protein expression profiles in systems with or without 0.5 × the minimum inhibitory concentration of ampicillin. RT-PCR was used to assess the transcriptional levels of the differentially expressed genes. Fifty-seven different proteins were induced or suppressed. The upregulated proteins were involved in transcription and translation, cell wall synthesis, bacterial SOS response, and detoxifying functions, and the downregulated proteins were involved in metabolism. These results indicated that a global response was induced in the recipient-donor co-culture system by the subinhibitory concentration of ampicillin. Further analysis revealed that a global regulatory network based on key pathways was induced in the system in response to the antibiotic pressure. These findings provide a new, more comprehensive view for research on antibiotic-resistance mechanisms in recipient-donor co-culture.

Antibiotics: An overview on the environmental occurrence, toxicity, degradation, and removal methods

“…To achieve the sustainable development, the future research directions are suggested as follows. (1) The development of analytical methods for the detection of a greater number of antibiotics and their TPs, in order to improve risk assessments and establish quality standards for antibiotics in the environment; (2) further toxicological studies must be performed on potential chronic effects of antibiotic mixtures and their degradation products at environment-related concentration; (3) understand better and deeper the toxicity of antibiotics toward non-target organisms by proteomic responses and gene expression study; (4) human health may be affected by the consumption of agricultural products polluted by antibiotics, more attention should be paid to the indirect effects of antibiotics caused by the drinking water and food chain; (5) further research is needed to evaluate the relationship between antibiotic contamination and the presence of ARGs/ARB in the environment; (6) elucidation of degradation products and their bioactivity needs to be further investigated; (7) develop efficient and low-cost treatment technology for controlling the emission of antibiotic contaminants.…”

“…Antibiotics have been extensively used for the treatment and prevention of diseases in humans, as well as for protecting animal health and promoting animal growth in aquaculture and livestock industries [ 2 , 7 ]. Recently, due to the global growth in human population and rising demand for animal protein [ 2 ], antibiotic consumption has been growing and reached enormous amounts.…”

The problem of resistance of microorganisms ‒ challenge to humanity

“…Такі АМП як триметоприм та хінолони інгібують дію ферментів, що взаємодіють із ДНК, і блокують процес реплікації. Мутації у клітинах Escherichia colі зумовлюють також ампіцилін та канаміцин, стимулюючи утворення ROS-продуктів [22,[24][25][26].…”

Bacteria From the Multi-Contaminated Tinto River Estuary (SW, Spain) Show High Multi-Resistance to Antibiotics and Point to Paenibacillus spp. as Antibiotic-Resistance-Dissemination Players

“…This function was demonstrated only in a few cases (Currie et al, 1999;Neeno-Eckwall et al, 2001;Haas and Défago, 2005). Antibiotic concentrations in nature may not be enough for this purpose (Fajardo et al, 2009), while low concentrations have pleiotropic effects that are not related to competition (Davies et al, 2006;Linares et al, 2006;Yim et al, 2007;Fajardo and Martinez, 2008;Sengupta et al, 2013;Andersson and Hughes, 2014;Goneau et al, 2015;Martínez, 2017;Xiong et al, 2017). However, low individual concentrations of a cocktail of antibiotics could have a combined effect [reviewed by Danner et al (2019)].…”