Proteomic analysis reveals rattlesnake venom modulation of proteins associated with cardiac tissue damage in mouse hearts

Santos, Wellington da Silva; Montoni, Fabio; Eichler, Rosangela Aparecida dos Santos; Arcos, Stephanie Santos Suehiro; Andreotti, Diana Zukas; Kisaki, Carolina Yukiko; Evangelista, Kimberly Borges; Calacina, Hamida Macêdo; Lima, Ismael Feitosa; Soares, Magna Aparecida Maltauro; Gren, Eric C.K.; Carvalho, Valdemir Melechco; Ferro, Emer S.; Nishiyama-Jr, Milton Yutaka; Chen, Zhibin; Iwai, Leo Kei

doi:10.1016/j.jprot.2022.104530

Cited by 6 publications

(1 citation statement)

References 79 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Snake venoms are complex toxic mixtures whose summative and synergistic effects trigger a cascade of physiological disruptions. Recent proteomic studies have investigated the effects of venoms or isolated toxins on mouse plasma, 12 plasma extracellular vesicles, 13 wound exudate, 14–17 and cardiac tissue, 18 each providing insight into the specific biological processes activated during envenomation. Bridging the gap between the data gathered in model organisms under experimental conditions and the information acquired in a clinical setting has the potential to provide a comprehensive picture of the mechanisms of pathophysiology triggered by envenomation as well as a suitable panel of potential biomarkers indicative of envenomation syndromes and outcomes.…”

Section: Introductionmentioning

confidence: 99%

Feasibility of detecting snake envenomation biomarkers from dried blood spots

Smith

Brandehoff

Pepin

et al. 2023

Analytical Science Advances

View full text Add to dashboard Cite

Biofluid proteomics is a sensitive and high throughput technique that provides vast amounts of molecular data for biomarker discovery. More recently, dried blood spots (DBS) have gained traction as a stable, noninvasive, and relatively cheap source of proteomic data for biomarker identification in disease and injury. Snake envenomation is responsible for significant morbidity and mortality worldwide; however, much remains unknown about the systemic molecular response to envenomation and acquiring biological samples for analysis is a major hurdle. In this study, we utilized DBS acquired from a case of lethal rattlesnake envenomation to determine the feasibility of discovering biomarkers associated with human envenomation. We identified proteins that were either unique or upregulated in envenomated blood compared to non‐envenomated blood and evaluated if physiological response pathways and protein markers that correspond to the observed syndromes triggered by envenomation could be detected. We demonstrate that DBS provide useful proteomic information on the systemic processes that resulted from envenomation in this case and find evidence for a massive and systemic inflammatory cascade, combined with coagulation dysregulation, complement system activation, hypoxia response activation, and apoptosis. We also detected potential markers indicative of lethal anaphylaxis, cardiac arrest, and brain death. Ultimately, DBS proteomics has the potential to provide stable and sensitive molecular data on envenomation syndromes and response pathways, which is particularly relevant in low‐resource areas which may lack the materials for biofluid processing and storage.

show abstract