Proteomic analysis reveals different involvement of proteins during the maintenance and release of rice seed dormancy

Xu, Hao; Lian, Ling; Jiang, Minrong; Zhu, Yu Cheng; Wu, Fangxi; Jiang, Jiahuang; Zheng, Yanmei; Tong, Jianhua; Lin, Yuelong; Wang, Fuxiang; Xie, Hong; Chen, Liping; Xie, Huaan; Zhang, Chenglong

doi:10.1007/s11032-019-0963-x

Cited by 3 publications

(3 citation statements)

References 39 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…We also used the iTRAQ method (Xu et al, ) to combine our large‐scale rice seed proteome analysis with quantitative data on proteins modulated during rice seed ageing. Therefore, the expression profiles of ageing rice seed proteins were analysed using two complementary approaches: one‐dimensional electrophoresis (1‐DE) and LC‐MS/MS (Figure S1a).…”

Section: Resultsmentioning

confidence: 99%

Comparative analysis of different methods revealing the involvement of proteins during ageing of rice seeds

Jiang

Lian

et al. 2019

Plant Breeding

Self Cite

View full text Add to dashboard Cite

Seed longevity is a very important characteristic controlling seed quality. However, the mechanism underlying this characteristic is poorly understood. In this study, 'FH7185', a storable rice variety, the germination rate of which was 66.63%, much higher than the control after artificial ageing under relative humidity 88% and 42°C for 21 days. Different methods were applied to reveal the involvement of proteins during ageing of rice seeds. In total, 35 differential proteins were identified from 2D‐PAGE, and 3,719 proteins from iTRAQ analysis. A comparison of these two methods showed that not all protein types could be detected on the 2D‐PAGE gels, and the dynamic range was somewhat limited. So the comprehensive proteome map from the iTRAQ analysis was used to identify the quantitatively regulated proteins that played roles in seed longevity, and found that the most marked change was the increased abundance of many metabolic enzymes, especially the ones involved in α‐linolenic acid metabolism in the embryos during ageing. OsLOX2 and OsLOX3 had a negative effect on seed longevity, which were lower in FH7185 than the control. The dehydrogenase (LOC_Os07g44430) which played a major catalytic role in lipid peroxidation, also changed significantly during ageing. Therefore, OsLOX2, OsLOX3 and Loc_OS 07G44430 may be involved in the regulation of seed longevity with a synergistic effect.

show abstract

Section: Resultsmentioning

confidence: 99%

Comparative analysis of different methods revealing the involvement of proteins during ageing of rice seeds

Jiang

Lian

et al. 2019

Plant Breeding

Self Cite

View full text Add to dashboard Cite

show abstract

“…Plants acquire freezing tolerance by modifying their transcriptome, proteome, and metabolome (Chinnusamy et al, 2010;Xu et al, 2019;Brown et al, 2020). DIA-based proteomic data showed that the reduction in temperature during germination had a strong effect on the total soluble protein categories in the M. micrantha germ (Figure 4D).…”

Section: Discussionmentioning

confidence: 99%

Antioxidant Regulation and DNA Methylation Dynamics During Mikania micrantha Seed Germination Under Cold Stress

Cui

Wang

et al. 2022

Front. Plant Sci.

View full text Add to dashboard Cite

As a primary goal, adaptation to cold climate could expand an invasion range of exotic plants. Here, we aimed to explore the regulation strategy of M. micrantha seed development under cold stress through molecular physiology and multi-omics analysis. Significant increase of hydrogen peroxide, malondialdehyde, and electrolyte leakage observed under cold stress revealed that oxidative damage within M. micrantha seed cells was induced in the initial germination phase. Proteomic data underscored an activation of antioxidant activity to maintain redox homeostasis, with a cluster of antioxidant proteins identified. Genomic-wide transcriptome, in combination with time-series whole-genome bisulfite sequencing mining, elucidated that seven candidate genes, which were the target of DNA demethylation-dependent ROS scavenging, were possibly associated with an M. micrantha germ break. Progressive gain of CHH context DNA methylation identified in an early germination phrase suggested a role of a DNA methylation pathway, while an active DNA demethylation pathway was also initiated during late seed development, which was in line with the expression trend of methylation and demethylation-related genes verified through qRT-PCR. These data pointed out that cold-dependent DNA demethylation and an antioxidant regulatory were involved together in restoring seed germination. The expression level of total 441 genes presented an opposite trend to the methylation divergence, while the expression of total 395 genes was proved to be negatively associated with their methylation levels. These data provided new insights into molecular reprograming events during M. micrantha seed development.

show abstract

“…This causes delays in commercialization and subsequent sowing (Maiti et al, 2006;Vujaković et al, 2012) as well as an increase of commercial costs because of the extended seed storage (Bodrone et al, 2017). Dormancy release is associated with alterations in gene and protein expression, oxidative modifications of gene transcripts and proteins, and even epigenetic modifications; all this will ultimately cause changes in the physiological state of the seed (Iwasaki et al, 2019;Xu et al, 2019). Thus, the study of the metabolic and signaling pathways of phytohormones, as well as the understanding of their potential interactions in the maintenance and release of dormancy, becomes fundamental.…”

Section: Introductionmentioning

confidence: 99%

The interplay of specific hormonal profile in fruit parts of sunflower inbred lines with contrasting dormancy levels during germination and dormancy breaking by exogenous application of plant growth regulators

Bel¹,

Andrade²,

Maddalena

et al. 2023

Crop Science

View full text Add to dashboard Cite

Sunflower (Helianthus annuus L.) seed dormancy at harvest is an undesirable characteristic regarding productive systems. From a commercial point of view, the dormancy generates production and marketing problem with a negative impact on crop yields. To overcome this problem, we studied different treatments, including the application of growth regulators. In addition, as the interaction of various hormones in different sunflower fruit parts is not fully understood regarding regulation of dormancy and germination processes, we also (1) characterized the dormancy level of sunflower seeds of Xi3 and X274 inbred lines; (2) assessed the effect of exogenous growth regulators on dormancy release; (3) evaluated the endogenous content of abscisic acid (ABA), gibberellins (GAs), 12-oxo-phytodienoic acid (OPDA), jasmonic acid (JA), salicylic acid (SA), auxins, and cytokinins (CKs) in embryonic axis, cotyledons, pericarp, and seed coat-endosperm; and (4) analyzed the kinetics of the accumulation of these phytohormones in Xi3 embryonic axis during early imbibition (3 to 12 h) of cypselas treated, or not, with growth regulators effective in the dormancy release. We were able to classify the two sunflower lines studied according to dormancy level: The Xi3 line was dormant and X274 line was non-dormant at harvest. The results showed that the dormancy of Xi3 seeds is associated with a high level of ABA, OPDA, and JA, while germination of X274 dry seeds at harvest time is related to high levels of GA 1 . Exogenous treatment with GA 3 and Ethephon overcame Xi3 seed dormancy through the changes in endogenous hormonal profiles of the embryonic axis. The exogenous application of GA 3 induced JA, SA, and CKs accumulation and to modify the ABA/GA 1 ratio, whereas Ethephon treatments mediated

show abstract

Proteomic analysis reveals different involvement of proteins during the maintenance and release of rice seed dormancy

Cited by 3 publications

References 39 publications

Comparative analysis of different methods revealing the involvement of proteins during ageing of rice seeds

Comparative analysis of different methods revealing the involvement of proteins during ageing of rice seeds

Antioxidant Regulation and DNA Methylation Dynamics During Mikania micrantha Seed Germination Under Cold Stress

The interplay of specific hormonal profile in fruit parts of sunflower inbred lines with contrasting dormancy levels during germination and dormancy breaking by exogenous application of plant growth regulators

Contact Info

Product

Resources

About