Proteomic analysis on zoxamide-induced sensitivity changes in Phytophthora cactorum

Mei, Xinyue; Yang, Min; Jiang, Bingbing; Ding, Xuezhi; Deng, Weiping; Dong, Yue; Chen, Lei; Liu, Xili; Zhu, Shusheng

doi:10.1016/j.pestbp.2015.01.012

Cited by 7 publications

(8 citation statements)

References 42 publications

(65 reference statements)

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“…We identified the ATP-binding cassette (ABC) transporter families and major facilitator superfamily (MFS), as well as the cytochrome P450 (CYPs), peroxidase (POD), glutathione S-transferases (GSTs), methyltransferase (MTR), and dehydrogenase in the P. cactorum genome (Table 2 ). Our previous proteomic analysis also showed that P. cactorum employed detoxification enzymes to tolerate a series of fungicides 13 . These data imply that the expansion of detoxification metabolism genes enable P. cactorum with higher detoxification ability against host defense compounds or commercial fungicides.…”

Section: Resultsmentioning

confidence: 91%

“…3 ). Previous study also showed that P. cactorum had a stronger ability to obtain fungicide tolerance when being cultured in increasing concentrations of fungicide 13 . Proteomic analysis revealed that many proteins involved in the detoxification metabolic pathway are responsible for the tolerance of P. cactorum to fungicides 14 .…”

Section: Introductionmentioning

confidence: 82%

“…Proteomic analysis revealed that many proteins involved in the detoxification metabolic pathway are responsible for the tolerance of P. cactorum to fungicides 14 . Over the past decades, it is apparent that P. cactorum has gradually developed tolerance to many fungicides in the field 9 , 13 , 15 – 17 . The above described data implied that P. cactorum had a high ability to detoxify plant defense compounds or fungicides.…”

Section: Introductionmentioning

confidence: 99%

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The Phytophthora cactorum genome provides insights into the adaptation to host defense compounds and fungicides

Yang

Duan

Mei

et al. 2018

Sci Rep

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Phytophthora cactorum is a homothallic oomycete pathogen, which has a wide host range and high capability to adapt to host defense compounds and fungicides. Here we report the 121.5 Mb genome assembly of the P. cactorum using the third-generation single-molecule real-time (SMRT) sequencing technology. It is the second largest genome sequenced so far in the Phytophthora genera, which contains 27,981 protein-coding genes. Comparison with other Phytophthora genomes showed that P. cactorum had a closer relationship with P. parasitica, P. infestans and P. capsici. P. cactorum has similar gene families in the secondary metabolism and pathogenicity-related effector proteins compared with other oomycete species, but specific gene families associated with detoxification enzymes and carbohydrate-active enzymes (CAZymes) underwent expansion in P. cactorum. P. cactorum had a higher utilization and detoxification ability against ginsenosides–a group of defense compounds from Panax notoginseng–compared with the narrow host pathogen P. sojae. The elevated expression levels of detoxification enzymes and hydrolase activity-associated genes after exposure to ginsenosides further supported that the high detoxification and utilization ability of P. cactorum play a crucial role in the rapid adaptability of the pathogen to host plant defense compounds and fungicides.

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Section: Resultsmentioning

confidence: 91%

Section: Introductionmentioning

confidence: 82%

Section: Introductionmentioning

confidence: 99%

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The Phytophthora cactorum genome provides insights into the adaptation to host defense compounds and fungicides

Yang

Duan

Mei

et al. 2018

Sci Rep

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“…An understanding of the molecular mechanisms by which resistance is conferred will be of critical value to growers, breeders, and scientists, as well as a thorough understanding of the pathogen populations. (Hill & Hausbeck, 2008;Jeffers et al, 2004;Marin et al, 2021Marin et al, , 2022Marin et al, , 2023Mei et al, 2015). Generally, they can provide sufficient protection when applied alone.…”

Section: Resistance In Cropsmentioning

confidence: 99%

The devastating oomycete phytopathogen Phytophthora cactorum: Insights into its biology and molecular features

Chen

Wen

Zhou

et al. 2023

Molecular Plant Pathology

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Phytophthora cactorum is one of the most economically important soilborne oomycete pathogens in the world. It infects more than 200 plant species spanning 54 families, most of which are herbaceous and woody species. Although traditionally considered to be a generalist, marked differences of P. cactorum isolates occur in degree of pathogenicity to different hosts. As the impact of crop loss caused by this species has increased recently, there has been a tremendous increase in the development of new tools, resources, and management strategies to study and combat this devastating pathogen. This review aims to integrate recent molecular biology analyses of P. cactorum with the current knowledge of the cellular and genetic basis of its growth, development, and host infection. The goal is to provide a framework for further studies of P. cactorum by highlighting important biological and molecular features, shedding light on the functions of pathogenicity factors, and developing effective control measures.TaxonomyP. cactorum (Leb. & Cohn) Schröeter: kingdom Chromista; phylum Oomycota; class Oomycetes; order Peronosporales; family Peronosporaceae; genus Phytophthora.Host rangeInfects about 200 plant species in 154 genera representing 54 families. Economically important host plants include strawberry, apple, pear, Panax spp., and walnut.Disease symptomsThe soilborne pathogen often causes root, stem, collar, crown, and fruit rots, as well as foliar infection, stem canker, and seedling damping off.

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“…Therefore, the most commonly implemented measures to control pepper blight are applying chemical fungicides, such as treating the soil with methyl bromide or metalaxyl, or spraying metalaxyl or metalaxyl on plants. Unfortunately, due to the long-term use and highly variable nature of P. capsici , some strains had developed resistance or tolerance to these fungicides ( Lamour & Hausbeck, 2000 ; Parra & Ristaino, 2001 ; Silvar, Merino & Dilv, 2006 ; Dunn et al, 2010 ), and there is a limited number of fungicides that can be used against the pathogen ( Mei et al, 2015 ; Pang et al, 2015 ). So, it is urgent to find more environmentally friendly and safe botanical fungicides.…”

Section: Introductionmentioning

confidence: 99%

Cinnamaldehyde inhibits the growth of Phytophthora capsici through disturbing metabolic homoeostasis

Wang

et al. 2021

PeerJ

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Background Phytophthora capsici Leonian (P. capsici) can cause wilting and roots rotting on pepper and other cash crops. The new fungicide cinnamaldehyde (CA) has high activity against this pathogen. However, its potential mechanism is still unknown. Methods In order to gain insights into the mechanism, isobaric tags for relative and absolute quantification (iTRAQ)-based quantitative proteomics was used to analyze P. capsici treated with CA. The iTRAQ results were evaluated by parallel reaction monitoring (PRM) analysis and quantitative real-time PCR (qRT-PCR) analysis. Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis was used to speculate the biochemical pathways that the agent may act on. Results The results showed that 1502 differentially expressed proteins were identified, annotated and classified into 209 different terms (like metabolic process, cellular process, single-organism process) based on Gene Ontology (GO) functional enrichment analysis and nine different pathways (glyoxylate and dicarboxylate metabolism, fatty acid metabolism and so on) based on the Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis. This study suggested that CA disordered fatty acid metabolism, polysaccharide metabolism and leucine metabolism. Based on PRM analysis, five proteins including CAMK/CAMK1 protein kinase, glucan 1,3-beta-glucosidase, 1,3-beta-glucanosyltransferase, methylcrotonoyl-CoA carboxylase subunit alpha and isovaleryl-CoA dehydrogenase were down-regulated in P. capsici treated with CA. Furthermore, the qRT-PCR analysis showed that the gene expression level of the interested proteins was consistent with the protein expression level, except for CAMK/CAMK1 protein kinase, acetyl-CoA carboxylase and fatty acid synthase subunit alpha. Conclusions CA destroyed the metabolic homoeostasisof P. capsici, which led to cell death. This is the first proteomic analysis of P. capsici treated with CA, which may provide an important information for exploring the mechanism of the fungicide CA against P. capsici.

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Proteomic analysis on zoxamide-induced sensitivity changes in Phytophthora cactorum

Cited by 7 publications

References 42 publications

The Phytophthora cactorum genome provides insights into the adaptation to host defense compounds and fungicides

The Phytophthora cactorum genome provides insights into the adaptation to host defense compounds and fungicides

The devastating oomycete phytopathogen Phytophthora cactorum: Insights into its biology and molecular features

Cinnamaldehyde inhibits the growth of Phytophthora capsici through disturbing metabolic homoeostasis

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