Proteomic analysis on roots of Oenothera glazioviana under copper-stress conditions

Abstract

Proteomic studies were performed to identify proteins involved in the response of Oenothera glazioviana seedlings under Cu stress. Exposure of 28-d-old seedlings to 50 μM CuSO4 for 3 d led to inhibition of shoot and root growth as well as a considerable increase in the level of lipid peroxidation in the roots. Cu absorbed by O. glazioviana accumulated more easily in the root than in the shoot. Label-free proteomic analysis indicated 58 differentially abundant proteins (DAPs) of the total 3,149 proteins in the roots of O. glazioviana seedlings, of which 36 were upregulated and 22 were downregulated under Cu stress conditions. Gene Ontology analysis showed that most of the identified proteins could be annotated to signal transduction, detoxification, stress defence, carbohydrate, energy, and protein metabolism, development, and oxidoreduction. We also retrieved 13 proteins from the enriched Kyoto Encyclopaedia of Genes and Genomes and the protein-protein interaction databases related to various pathways, including the citric acid (CA) cycle. Application of exogenous CA to O. glazioviana seedlings exposed to Cu alleviated the stress symptoms. Overall, this study provided new insights into the molecular mechanisms of plant response to Cu at the protein level in relation to soil properties.

RETRACTED: Morpho-Physiological and Proteomic Analyses of Eucalyptus camaldulensis as a Bioremediator in Copper-Polluted Soil in Saudi Arabia

“…Furthermore, quantitative analysis showed that relative water content, chlorophyll content, shoot and root fresh weight, and shoot and root dry weight of seedlings treated with the highest dose (100 µM) were significantly reduced by 33.3%, 54.8%, 36.5%, 42.5%, 40.7%, and 33.3% respectively, relative to those of the control (Table 1). On the other hand, similar trends in observations and reductions were noticed for shoot and root fresh weight and shoot and root dry weight for O. glazioviana in response to Cu stress [27]. The perturbation in the biomass production in both shoot and root might be linked to the effect of Cu on cell division, which retarded normal cell growth and development [39].…”

“…Plants react differentially to heavy metal stresses according to their species, the metal type, and metal concentration [ 27 ]. Therefore, the molecular mechanisms of plant responses are needed using proteomic studies under Cu stress.…”

Influence of Heavy Metals (Ni, Cu, and Zn) on Nitro-Oxidative Stress Responses, Proteome Regulation and Allergen Production in Basil (Ocimum basilicum L.) Plants

“…Ribosomal protein L14 is one of many proteins that form a large ribosomal subunit (60S) and is considered to play a main role in the ribonucleoprotein complex ( Davies et al, 1996 ), while ribulose-1,5-bisphosphate carboxylase/oxygenase (also known as RuBisCO) is a key enzyme involved in the first step of carbon assimilation during the Calvin cycle ( Spreitzer and Salvucci, 2002 ). This observed upregulation comes in agreement with several proteomic analyses of plants under Cu contamination, which identified similar protein components to be induced ( Ahsan et al, 2007 ; Hego et al, 2016 ; Wang et al, 2017 ), likely in an attempt by the plants to fuel defense mechanisms in response to stress imposition via increased primary metabolism pathways.…”

Excess Copper-Induced Alterations of Protein Profiles and Related Physiological Parameters in Citrus Leaves

“…Recently, there have been several reports investigating Cu toxicity responsive proteins. Most reports, however, have focused on herbaceous plants, including rice [17][18][19], Allium cepa [20], Oenothera glazioviana [21], Arabidopsis [22], Cannabis sativa [23], Agrostis capillaris [24], Elsholtzia splendens [25,26], sorghum [27,28] and wheat [29], while only one study investigated Cu-toxic effects on protein profiles in leaves of woody plant Eucalyptus camaldulensis [30]. Also, most of the above studies mainly focused on Cu-toxicity-responsive proteins occurring in roots because Cu is preferentially accumulated in Cu-stressed roots, while only few studies investigated Cu-toxic effects on protein profiles in leaves [25,27,29,30].…”