Proteomic analysis of whole saliva in chronic periodontitis

Hartenbach, Fátima Aparecida Rocha Resende; Velásquez, Erika; Nogueira, Fábio César Sousa; Domont, Gilberto B.; Ferreira, Eliane de Oliveira; Colombo, Ana Paula Vieira

doi:10.1016/j.jprot.2019.103602

Cited by 36 publications

(37 citation statements)

References 54 publications

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“…In another study, Hartenbach et al, using a hybrid mass spectrometer LTQ Orbitrap Velos for proteomic analysis to obtain greater resolution, mass accuracy and sensitivity, were able to improve the qualitative and quantitative analyses of the salivary proteome (Hartenbach et al, 2020). The authors showed that a large range of salivary proteins with protective functions and associated with oral homeostasis were down-regulated in chronic periodontitis patients compared to individuals presenting periodontal health.…”

Section: Proteomics and Periodontitismentioning

confidence: 99%

Dysbiosis From a Microbial and Host Perspective Relative to Oral Health and Disease

et al. 2021

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The significance of microbiology and immunology with regard to caries and periodontal disease gained substantial clinical or research consideration in the mid 1960's. This enhanced emphasis related to several simple but elegant experiments illustrating the relevance of bacteria to oral infections. Since that point, the understanding of oral diseases has become increasingly sophisticated and many of the original hypotheses related to disease causality have either been abandoned or amplified. The COVID pandemic has reminded us of the importance of history relative to infectious diseases and in the words of Churchill “those who fail to learn from history are condemned to repeat it.” This review is designed to present an overview of broad general directions of research over the last 60 years in oral microbiology and immunology, reviewing significant contributions, indicating emerging foci of interest, and proposing future directions based on technical advances and new understandings. Our goal is to review this rich history (standard microbiology and immunology) and point to potential directions in the future (omics) that can lead to a better understanding of disease. Over the years, research scientists have moved from a position of downplaying the role of bacteria in oral disease to one implicating bacteria as true pathogens that cause disease. More recently it has been proposed that bacteria form the ecological first line of defense against “foreign” invaders and also serve to train the immune system as an acquired host defensive stimulus. While early immunological research was focused on immunological exposure as a modulator of disease, the “hygiene hypothesis,” and now the “old friends hypothesis” suggest that the immune response could be trained by bacteria for long-term health. Advanced “omics” technologies are currently being used to address changes that occur in the host and the microbiome in oral disease. The “omics” methodologies have shaped the detection of quantifiable biomarkers to define human physiology and pathologies. In summary, this review will emphasize the role that commensals and pathobionts play in their interaction with the immune status of the host, with a prediction that current “omic” technologies will allow researchers to better understand disease in the future.

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Section: Proteomics and Periodontitismentioning

confidence: 99%

Dysbiosis From a Microbial and Host Perspective Relative to Oral Health and Disease

et al. 2021

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“…The presence of proteins biomarkers related to neutrophil-secreted proteins, cellular proliferation, migration, and adhesion are associated with aggressive periodontitis [104]. Recently, Hartenbach et al [116] reported data about the salivary proteomic analysis of chronic periodontitis patients (Figure 3a,b). In periodontitis patients, the salivary acidic proline rich phosphoproteins, a submandibular salivary gland androgen regulated protein, and a cystatin were increased (Figure 3b).…”

Section: Proteomics and Peptidomics Approaches To The Diseasementioning

confidence: 99%

“…In periodontitis patients, the salivary acidic proline rich phosphoproteins, a submandibular salivary gland androgen regulated protein, and a cystatin were increased (Figure 3b). Among others, histatin-1, fatty acid binding protein, and thioredoxin were predominant in the diseased state [116]. Shin et al conducted deep sequencing using proteomic and detected S100A8 and S100A9 as potential biomarkers for periodontitis [117] (Figure 3c).…”

Section: Proteomics and Peptidomics Approaches To The Diseasementioning

confidence: 99%

An Evidence-Based Update on the Molecular Mechanisms Underlying Periodontal Diseases

Qasim

Alotaibi

Jasser

et al. 2020

IJMS

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Several investigators have reported about the intricate molecular mechanism underlying periodontal diseases (PD). Nevertheless, the role of specific genes, cells, or cellular mechanisms involved in the pathogenesis of periodontitis are still unclear. Although periodontitis is one of the most prevalent oral diseases globally, there are no pre-diagnostic markers or therapeutic targets available for such inflammatory lesions. A pivotal role is played by pro- and anti-inflammatory markers in modulating pathophysiological and physiological processes in repairing damaged tissues. In addition, effects on osteoimmunology is ever evolving due to the ongoing research in understanding the molecular mechanism lying beneath periodontal diseases. The aim of the current review is to deliver an evidence-based update on the molecular mechanism of periodontitis with a particular focus on recent developments. Reports regarding the molecular mechanism of these diseases have revealed unforeseen results indicative of the fact that significant advances have been made to the periodontal medicine over the past decade. There is integrated hypothesis-driven research going on. Although a wide picture of association of periodontal diseases with immune response has been further clarified with present ongoing research, small parts of the puzzle remain a mystery and require further investigations.

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“…The expression proteome combines the terms proteins and genes, and implies the simultaneous study of all proteins in a cell and protein complements of the genome (Parker et al, 2010). Studies in periodontology using proteomic analysis of whole saliva from chronic periodontitis patients were able to show the suppression of several salivary proteins related to protective functions and oral homeostasis (Hartenbach et al, 2020).…”

Section: Diagnostic Devices Salivary Proteome and Salivary Genomementioning

confidence: 99%

“…Studies in periodontology using proteomic analysis of whole saliva from chronic periodontitis patients were able to show the suppression of several salivary proteins related to protective functions and oral homeostasis (Hartenbach et al, 2020). In 2019, deep sequencing combined with rapid shotgun analysis for ultra-deep and quantitative saliva proteome characterization identified 5,500 host proteins and more than 2,000 microbial proteins in samples of periodontitis patients, with a strong correlation between dynamics of the oral microbiome and salivary proteins (Grassl et al, 2016).…”

Section: Diagnostic Devices Salivary Proteome and Salivary Genomementioning

confidence: 99%

Biosensor and Lab-on-a-chip Biomarker-identifying Technologies for Oral and Periodontal Diseases

et al. 2020

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Proteomic analysis of whole saliva in chronic periodontitis

Cited by 36 publications

References 54 publications

Dysbiosis From a Microbial and Host Perspective Relative to Oral Health and Disease

Dysbiosis From a Microbial and Host Perspective Relative to Oral Health and Disease

An Evidence-Based Update on the Molecular Mechanisms Underlying Periodontal Diseases

Biosensor and Lab-on-a-chip Biomarker-identifying Technologies for Oral and Periodontal Diseases

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