Proteomic Analysis of Undiluted Lung Epithelial Lining Fluid

Kipnis, Éric; Hansen, Kirk C.; Sawa, Teiji; Moriyama, Kiyoshi; Zurawel, Ashley; Ishizaka, Akitoshi; Wiener-Kronish, Jeanine P.

doi:10.1378/chest.07-1643

Cited by 29 publications

(20 citation statements)

References 50 publications

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“…Indeed, an alternative sample, directly sampled epithelial lining fluid, rather than indirectly recovered and diluted under the form of BAL fluid, has recently been obtained by bronchoscopic use of a specially designed apparatus, the bronchoscopic microsampling probe, or BMS probe [36]. In a preliminary study, we successfully retrieved undiluted epithelial lining fluid from the BMS probe tip and submitted it to proteomic characterization [37]. We found that, although we used an experimental animal (rabbit) that has incomplete databases for protein identification and low protein loads, we identified more (43 versus 30) non-redundant proteins than Bow- [20] who used high protein loads and complete human genome derived databases.…”

Section: Future Directions Epithelial Lining Fluidmentioning

confidence: 99%

Lung Proteomics in Intensive Care

Kipnis¹,

Hansen²

2009

Yearbook of Intensive Care and Emergency Medicine

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Section: Future Directions Epithelial Lining Fluidmentioning

confidence: 99%

Lung Proteomics in Intensive Care

Kipnis¹,

Hansen²

2009

Yearbook of Intensive Care and Emergency Medicine

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“…After the removal of the cells, the resulting BALF contains only the proteins that are soluble in the saline solution [41] and may also contain components due to irritation of the airways induced by lavage. For this reason, BALF may be considered as a diluted solution of the ELF [42].…”

Section: Bal and Balfmentioning

confidence: 99%

Asthma and COPD proteomics: Current approaches and future directions

Terracciano

Pelaia

Preianò

et al. 2015

Proteomics Clinical Apps

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Although asthma and chronic obstructive pulmonary disease COPD represent the two most common chronic respiratory diseases worldwide, the mechanisms underlying their pathobiology need to be further elucidated. Presently, differentiation of asthma and COPD are largely based on clinical and lung function parameters. However, the complexity of these multifactorial diseases may lead to misclassification and to inappropriate management strategies. Recently, tremendous progress in MS has extended the sensitivity, accuracy, and speed of analysis, enabling the identification of thousands of proteins per experiment. Beyond identification, MS has also greatly implemented quantitation issues allowing to assess qualitative-quantitative differences in protein profiles of different samples, in particular diseased versus normal. Herein, we provide a summary of recent proteomics-based investigations in the field of asthma/COPD, highlighting major issues related to sampling and processing procedures for proteomic analyses of specific airway and parenchymal specimens (induced sputum, exhaled breath condensate, epithelial lining fluid, bronchoalveolar and nasal lavage fluid), as well as blood-derived specimen (plasma and serum). Within such a context, together with current difficulties and limitations mainly due to lack of general standardization in preanalytical sampling procedure, our discussion will focus on the challenges and possible benefits of proteomic studies in phenotypic stratification of asthma and COPD.

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“…Benchmark proteomic studies in the form of so-called 'shotgun' analyses have been used to generate proteome lists for many compartments of the airways, including a number of biofluids, including saliva [39,40], BALF (BAL fluid) [41,42], nasal lavage fluid/mucus [43,44], epithelial lining fluid [45] and sputum [46]. Such studies are beginning to enable comparisons of proteomes between different airways compartments and different diseases ( Figure 2).…”

Section: Proteomics Of Induced Sputummentioning

confidence: 99%

Induced sputum: a window to lung pathology

Nicholas

Djukanović

2009

Biochemical Society Transactions

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Sputum is recognized as a sampling method for the monitoring and assessment of chronic lung diseases such as asthma, COPD (chronic obstructive pulmonary disease) and cystic fibrosis. Sputum samples the central airways and its protein components (e.g. mucins and cytokines), cellular components (e.g. eosinophils and neutrophils) and microbiological components (e.g. viruses and bacteria) can be used as markers of disease severity, exacerbation, susceptibility or progression. This paper describes the basic constituents of induced sputum and how these influence the quantification and identification of novel biomarkers of chronic lung diseases using techniques such as proteomics.

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Proteomic Analysis of Undiluted Lung Epithelial Lining Fluid

Cited by 29 publications

References 50 publications

Lung Proteomics in Intensive Care

Lung Proteomics in Intensive Care

Asthma and COPD proteomics: Current approaches and future directions

Induced sputum: a window to lung pathology

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