Proteomic analysis of Tunisian grapevine cultivar Razegui under salt stress

Jellouli, Neila; Jouira, Hatem Ben; Skouri, Houda; Ghorbel, Abdelwahed; Gourgouri, Ali; Mliki, Ahmed

doi:10.1016/j.jplph.2007.02.009

Cited by 72 publications

(60 citation statements)

References 49 publications

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“…For example, a soybean PR-5-homologous gene (GmOLPa) was highly induced in soybean roots, stems and leaves after initiation of high-salt stress (Onishi et al, 2006). A PR protein was differentially expressed by salt stress in grapevine (Vitis vinifera) (Jellouli et al, 2008), and a PR gene was induced by salt stress in Arabidopsis (Seo et al, 2008), potato (Zhu et al, 1995;HmidaSayari et al, 2005), maize (Andjelkovic and Thompson, 2006) and Mesembryanthemum crystallinum (Kore-eda et al, 2004). Previous study has shown that overexpression of the PR gene conferred salt tolerance in transgenic plants (Srivastava et al, 2006).…”

Section: Discussionmentioning

confidence: 99%

Cloning and Characterization of a Pathogenesis-Related Gene (ThPR10) from Tamarix hispida

Zhang¹,

Wang²,

Li³

2010

Acta Biologica Cracoviensia Series Botanica

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A PR10 gene (ThPR10) was cloned from Tamarix hispida and characterized. Real-time RT-PCR was employed to evaluate gene expression levels. ThPR10 was expressed in both leaves and roots of T. hispida under normal growth conditions, and can be highly induced in both leaf and root tissues by abiotic stresses including NaCl, PEG, cold, CdCl 2 , and ABA (abscisic acid) treatments. Our results indicated that ThPR10 is involved in the abiotic stress response, and regulated by an ABA-dependent signaling pathway. Subsequently, ThPR10 was localized at the subcellular level. The gene was fused with the GFP N-terminal driven by CaMV35S promoter and transiently expressed in onion epidermal cells. This strategy localized the ThPR10 protein to the nucleus of onion epidermal cells, suggesting that the pathogenesis-related proteins play a functional role in the cell nucleus.K Ke ey y w wo or rd ds s: : Abiotic stress, gene expression, pathogenesis-related protein, subcellular location, Tamarix hispida. ACTA BIOLOGICA CRACOVIENSIA Series

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Section: Discussionmentioning

confidence: 99%

Cloning and Characterization of a Pathogenesis-Related Gene (ThPR10) from Tamarix hispida

Zhang¹,

Wang²,

Li³

2010

Acta Biologica Cracoviensia Series Botanica

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“…15 This leaf proteome map was used to analyze quantitative variations in leaf proteins during nitrogen mobilization revealing that 40% of the proteins displayed significant changes in their abundance during leaf development. 15 Vitis vinifera proteome has been studied extensively 4,[16][17][18][19][20][21][22][23] compared to muscadine proteome. Muscadine grapes have distinct morphology and significantly differ from Vitis vinifera in berry characteristics, composition and stress tolerance level.…”

Section: Dovepressmentioning

confidence: 99%

Proteome analysis of muscadine grape leaves

Basha

Katam²,

Vasanthaiah

et al. 2009

IJWR

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Muscadine grapes are native to the southeastern United States and are used for making wine and consumed as fresh fruit. Grape berries, as 'sink organs,' rely on the use of available carbohydrate resources produced by photosynthesis to support their development and composition. A high throughput two-dimensional gel electrophoresis (2-DE) was conducted on muscadine (Vitis rotundifolia) grape leaf proteins to document complexity in their composition and to determine protein identity and function for enhancing photosynthetic efficiency of muscadine grape. 2-DE resolved muscadine leaf proteins into 258 polypeptides with pIs between 3.5 and 8.0 and molecular weight between 12,000 to 15,0000 Daltons. The consistently expressed proteins were excised and subjected to sequencing. Homology search of protein sequences showed 84% identity with Viridi plantae database. Identity of some of these proteins included RuBisCO, glutamine synthetase, pathogenesis-related protein, glyoxisomal malate dehydrogenase, ribonucleoprotein, chloroplast precursor, oxygen evolving enhancer protein. Comparative analysis of 10 muscadine cultivars showed quantitative differences in expression of 39 polypeptides among these genotypes. The results suggested that the polypeptide composition of muscadine grape leaf is complex, and polypeptide number and amount vary widely among muscadine genotypes, and these variations may be responsible for differences in their physiology, berry and stress tolerance characteristics.

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“…Being the most widely cultivated perennial plant, grapevine occupies a privileged position and has the greatest economic value worldwide, as well as in Tunisia. However, autochthonous Tunisian grapevine varieties exhibit enhanced osmotic tolerance and adaptive responses to stress conditions [1][2] which represent a valuable resource for studying mechanisms of adaptation to salinity and drought in V. vinifera. Thus understanding the molecular genetic basis of the environmental stress responses would be crucial towards improving grapevines production.…”

Section: Introductionmentioning

confidence: 99%

Molecular characterization and in silico analysis of an alkaline α-galactosidase gene (Vv-α-gal/SIP) in grapevines (Vitis vinifera. L)

Daldoul¹,

Hanana²,

Mliki³

2012

Turk J Bioch

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Aim: After identifying and isolating a grapevine alkaline α-galactosidase gene and before initializing functional genomic studies, we judged necessary to acquire minimal knowledge about this enzyme that catalyzes galactose release in the more neutral to alkaline cytoplasm. Our goal was to search and identify structural motives and domains that could provide knowledge and information about protein activity and functionality. Methods: We realized a bioinformatics analysis using several tools based on the homology search, promoter analysis, in order to determine basic characteristics of this protein that could guide us in the functional characterization works. As with several bioinformatics approaches, it is the combination of tools and data resources which provides the best results. Results: We discovered a new alkaline α-gal gene by using Bioinformatics tools. Vv-α-gal/ SIP gene is made of 13 exons and measures 2.9 kb, it encodes a protein of 774 amino acids with a predicted molecular mass of 85 kDa. The enzyme contains several putative abiotic stress regulatory elements. Conclusion: This study reports the first bioinformatic characterization of the Vv-α-gal-SIP gene. We investigated the possibility of predicting the function of Vv-α-gal-SIP only by its sequence. Several regulatory elements were predicted and described to be involved in abiotic stress signaling. The discovery of new alkaline α-Gals may contribute to important technological applications of α-Gals.

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Proteomic analysis of Tunisian grapevine cultivar Razegui under salt stress

Cited by 72 publications

References 49 publications

Cloning and Characterization of a Pathogenesis-Related Gene (ThPR10) from Tamarix hispida

Cloning and Characterization of a Pathogenesis-Related Gene (ThPR10) from Tamarix hispida

Proteome analysis of muscadine grape leaves

Molecular characterization and in silico analysis of an alkaline α-galactosidase gene (Vv-α-gal/SIP) in grapevines (Vitis vinifera. L)

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