Proteomic analysis of tree peony (Paeonia ostii ‘Feng Dan’) seed germination affected by low temperature

Ren, Xiuxia; Xue, Jingqi; Wang, Shunli; Xue, Yuqian; Zhang, Ping; Jiang, Haidong; Zhang, Xiuxin

doi:10.1016/j.jplph.2017.12.016

Cited by 32 publications

(23 citation statements)

References 52 publications

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“…The chlorophyll level was measured by Plus Chlorophyll Meter (Spectrum technologies, Wales, UK). The contents of starch and soluble sugars were measured by the Anthrone colorimetric method according to Ren et al [ 86 ]. Soluble proteins were extracted with a sodium phosphate buffer and measured colorimetrically using a Bradford Reagent (Sigma-Aldrich, St. Louis, MO, USA) according to the manual.…”

Section: Methodsmentioning

confidence: 99%

Supplementary Light Source Affects the Growth and Development of Codonopsis lanceolata Seedlings

Ren

Jeong

et al. 2018

IJMS

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Codonopsis lanceolata is widely used in traditional medicine and diets. However, there is no optimal protocol for the commercial production of C. lanceolata seedlings. This study was carried out to find the optimum supplementary light source for the production of C. lanceolata seedlings. Seedlings were grown for four weeks in a glasshouse with an average daily light intensity of 490 μmol·m−2·s−1 photosynthetic photon flux density (PPFD) coming from the sun and a 16-h daily supplementary lighting at 120 μmol·m−2·s−1 PPFD from either high-pressure sodium (HPS), metal halide (MH), far-red (FR), white LED (LED-w), or mixed (white: red: blue = 1:2:1) LEDs (LED-mix). The results showed that the greatest total biomass, stem diameter, ratio of shoot weight to shoot length, root biomass, and ratio of root weight to shoot weight were found in seedlings grown under supplementary LED-mix. Meanwhile, the stomatal properties and soluble sugar contents were improved for seedlings in LED-mix. The contents of starch, total phenols, and flavonoids were the greatest for seedlings in LED-w and LED-mix. The expression of photosynthetic proteins and genes in seedlings was also enhanced by LED-mix. Overall, these results suggest that LED-mix is advantageous to the photosynthetic potential and the accumulation of biomass, carbohydrates and secondary metabolites in C. lanceolata.

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Section: Methodsmentioning

confidence: 99%

Supplementary Light Source Affects the Growth and Development of Codonopsis lanceolata Seedlings

Ren

Jeong

et al. 2018

IJMS

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“…Their results showed that cold treatment activated the citrate cycle, amino acid and lipid metabolism, energy production, calcium signaling, and cell growth processes to promote dormancy release in tree peony. Ren et al (2018), using proteomics, analyzed the effects of low temperature (4 °C) on the germination of P. ostii 'Feng Dan' seeds: lowtemperature treatment delayed the degradation of starch and other metabolites in seeds, thus affecting other physiological metabolic processes such as glycolysis, resulting in inhibited seed germination. Based on the four stages of flower development (stage 1, flower-bud stage; stage 2, initiating bloom stage; stage 3, bloom stage; stage 4, decline stage) of P. lactiflora 'Zi Feng Yu', Wu et al (2018) conducted transcriptomic and proteomic studies to unearth the key gene APETALA2 related to flower shape.…”

Section: Proteomics Metabolomics and Other Omicsmentioning

confidence: 99%

Advances in molecular biology of Paeonia L.

Fan

Wang

Dong

et al. 2019

Planta

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Molecular biology can serve as a tool to solve the limitations of traditional breeding and cultivation techniques related to flower patterns, the improvement of flower color, and the regulation of flowering and stress resistance. These characteristics of molecular biology ensured its significant role in improving the efficiency of breeding and germplasm amelioration of Paeonia. This review describes the advances in molecular biology of Paeonia, including: (1) the application of molecular markers; (2) genomics, transcriptomics, proteomics, metabolomics, and microRNA studies; (3) studies of functional genes; and (4) molecular biology techniques. This review also points out select limitations in current molecular biology, analyzes the direction of Paeonia molecular biology research, and provides advice for future research objectives.

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“…Propagation by seeds is an important and widely used method for tree peonies, and it is also required for the breeding of new cultivars [5]. However, the seeds of the tree peony have deep epicotyl dormancy as well as hypocotyl dormancy [7,8], which is an adaptation that resulted from biological evolution to help seeds survive in adverse environments [9]. It normally takes more than 8–9 months for tree peony seeds to germinate without any treatment under natural conditions, and the survival percentage of the seedlings is very low, which delays the propagation process and has restricted the development of tree peony production [7,10].…”

Section: Introductionmentioning

confidence: 99%

“…It normally takes more than 8–9 months for tree peony seeds to germinate without any treatment under natural conditions, and the survival percentage of the seedlings is very low, which delays the propagation process and has restricted the development of tree peony production [7,10]. Therefore, seed dormancy breaking has been a hot topic for scientific research on the tree peony for decades [8,11,12]. The methods for breaking seed dormancy of the tree peony include exogenous gibberellin treatment, chilling sand treatment, and their combinations with specific gibberellin concentrations, chilling sand treatment temperatures, and treatment periods, respectively [7,13].…”

Section: Introductionmentioning

confidence: 99%

“…The methods for breaking seed dormancy of the tree peony include exogenous gibberellin treatment, chilling sand treatment, and their combinations with specific gibberellin concentrations, chilling sand treatment temperatures, and treatment periods, respectively [7,13]. It has been fully proven that hypocotyl and epicotyl dormancy of the tree peony should be broken separately using with different treatments [7,8].…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

A Two-Stage Culture Method for Zygotic Embryos Effectively Overcomes Constraints Imposed by Hypocotyl and Epicotyl Seed Dormancy in Paeonia ostii ‘Fengdan’

Ren

Jeong

2019

Plants

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The effect of the exogenous hormone and light quality on breaking hypocotyl and epicotyl dormancy was studied. The results showed that the greatest percentage of hypocotyl dormancy breaking was observed with the Murashige and Skoog (MS) medium supplemented with or without 1.0 mg·L−1 gibberellin 3 (GA3), while ABA and endosperm greatly inhibited hypocotyl dormancy breaking. This suggests that hypocotyl dormancy of the Paeonia ostii ‘Fengdan’ embryo could be easily overcome by removing constraints of the surrounding endosperm, and ABA may be one of the constraint factors contained in the endosperm. The percentage of epicotyl dormancy breaking was also greatly affected by the concentration of 6-benzylaminopurine (BA) and GA3. Compared to BA by itself, adding GA3 to the medium containing BA highly enhanced epicotyl dormancy breaking, with the greatest percentage of epicotyl dormancy breaking in MS medium supplemented with both 0.5 mg·L−1 BA and 0.5–1.0 mg·L−1 GA3. The percentage of hypocotyl and epicotyl dormancy breaking was also affected by light and its quality. Red light-emitting diodes (LEDs) had the same effect as a dark condition on the hypocotyl dormancy breaking, while blue LEDs and a combination of red and blue LEDs had a negative effect on the hypocotyl dormancy breaking. Unexpectedly, blue LEDs greatly enhanced, whereas red LEDs inhibited, epicotyl dormancy breaking. Conclusively, a two-stage culture method was recommended for breaking the hypocotyl and epicotyl dormancy: hypocotyl dormancy was broken first using the MS medium without any plant growth regulators in the dark (25 °C), and epicotyl dormancy was subsequently broken with the MS medium supplemented with both 1.0 mg·L−1 GA3 and 0.5 mg·L−1 BA under blue light.

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Proteomic analysis of tree peony (Paeonia ostii ‘Feng Dan’) seed germination affected by low temperature

Cited by 32 publications

References 52 publications

Supplementary Light Source Affects the Growth and Development of Codonopsis lanceolata Seedlings

Supplementary Light Source Affects the Growth and Development of Codonopsis lanceolata Seedlings

Advances in molecular biology of Paeonia L.

A Two-Stage Culture Method for Zygotic Embryos Effectively Overcomes Constraints Imposed by Hypocotyl and Epicotyl Seed Dormancy in Paeonia ostii ‘Fengdan’

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