Proteomic analysis of the U1 snRNP of Schizosaccharomyces pombe reveals three essential organism-specific proteins

Newo, Alain N. S.; Lützelberger, Martin; Bottner, C.; Wehland, Jürgen; Wissing, Josef; Jänsch, Lothar; Käufer, Norbert F.

doi:10.1093/nar/gkl1144

Cited by 13 publications

(15 citation statements)

References 34 publications

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“…In F. graminearum , 139 genes had at least one intron with altered splicing efficiency in the Fgsrp1 deletion mutant. One (FGRRES_01244) of these genes with splicing efficiency defects is orthologous to PRP39 that is an essential component of yeast spliceosome (Lockhart and Rymond, ; Newo et al ., ). Therefore, it is likely that FgSRP1 plays a role in intron splicing in F. graminearum .…”

Section: Discussionsupporting

confidence: 78%

The FgSRP1 SR‐protein gene is important for plant infection and pre‐mRNA processing in Fusarium graminearum

Zhang

Gao

Sun

et al. 2017

Environmental Microbiology

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The versatile functions of SR (serine/arginine-rich) proteins in pre-mRNA splicing and processing are modulated by reversible phosphorylation. Previous studies showed that FgPrp4, the only protein kinase among spliceosome components, is important for intron splicing and the FgSrp1 SR protein is phosphorylated at five conserved sites in Fusarium graminearum. In this study, we showed that the Fgsrp1 deletion mutant rarely produced conidia and caused only limited symptoms on wheat heads and corn silks. Deletion of FgSRP1 also reduced ascospore ejection and deoxynivalenol (DON) production. Interestingly, FgSRP1 had two transcript isoforms due to alternative splicing and both of them were required for its normal functions in growth and DON biosynthesis. FgSrp1 localized to the nucleus and interacted with FgPrp4 in vivo. Deletion of all four conserved phosphorylation sites but not individual ones affected the FgSRP1 function, suggesting their overlapping functions. RNA-seq analysis showed that the expression of over thousands of genes and splicing efficiency in over 140 introns were affected. Taken together, FgSRP1 is important for conidiation, and pathogenesis and alternative splicing is important for its normal functions. The FgSrp1 SR protein is likely important for pre-mRNA processing or splicing of various genes in different developmental and infection processes.

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Section: Discussionsupporting

confidence: 78%

The FgSRP1 SR‐protein gene is important for plant infection and pre‐mRNA processing in Fusarium graminearum

Zhang

Gao

Sun

et al. 2017

Environmental Microbiology

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“…The role of Snu71 remains elusive too. Newo et al [ 32 ] have analyzed the U1 snRNP of Schizosaccharomyces pombe and found no homolog of Snu71 although homologs of Prp40 and Luc7 were clearly present. However, they speculate that Usp107p may be a functional homolog of Snu71, given its similar size and the presence of a PWI domain in Usp107p.…”

Section: Discussionmentioning

confidence: 99%

The FF domains of yeast U1 snRNP protein Prp40 mediate interactions with Luc7 and Snu71

Ester

Uetz

2008

BMC Biochemistry

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Background: The FF domain is conserved across all eukaryotes and usually acts as an adaptor module in RNA metabolism and transcription. Saccharomyces cerevisiae encodes two FF domain proteins, Prp40, a component of the U1 snRNP, and Ypr152c, a protein of unknown function. The structure of Prp40, its relationship to other proteins within the U1 snRNP, and its precise function remain little understood.

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“…Fission yeast strains expressing TAP-Rrn7 and HA-Rrn11 were constructed as described in Newo et al (22).…”

Section: Methodsmentioning

confidence: 99%

Rrn7 Protein, an RNA Polymerase I Transcription Factor, Is Required for RNA Polymerase II-dependent Transcription Directed by Core Promoters with a HomolD Box Sequence

Rojas

Moreira-Ramos

Zock-Emmenthal

et al. 2011

Journal of Biological Chemistry

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The region in promoters that specifies the transcription machinery is called the core promoter, displaying core promoter elements (CPE) necessary for establishment of a preinitiation complex and the initiation of transcription. A classical CPE is the TATA box. In fission yeast, Schizosaccharomyces pombe, a new CPE, called HomolD box, was discovered. Collectively, 141 ribosomal protein genes encoding the full set of 79 different ribosomal proteins and more than 60 other housekeeping genes display a HomolD box in the core promoter. Here, we show that transcription directed by the HomolD box requires the RNA polymerase II machinery, including the general transcription factors. Most intriguingly, however, we identify, by DNA affinity purification, Rrn7 as the protein binding to the HomolD box. Rrn7 is an evolutionary conserved member of the RNA polymerase I machinery involved in transcription initiation of core ribosomal DNA promoters. ChIP shows that Rrn7 cross-links to a ribosomal protein gene promoter containing the HomolD box but not to a promoter containing a TATA box. Taken together, our results suggest that Rrn7 is an excellent candidate to be involved in the coordination of ribosomal DNA and ribosomal gene transcription during ribosome synthesis and, therefore, offer a new perspective to study conservation and evolvability of regulatory networks in eukaryotes.Ribosome biogenesis in eukaryotes is a highly coordinated process involving three different RNA polymerases. RNA polymerase III (RNAPIII) 3 synthesizes the small 5 S RNA. RNAPI synthesizes the large rRNA precursor. Transcription at ribosomal RNA core promoters is initiated by the TATA binding protein (TBP) and TBP-associated factors (TAF 1 s) forming a preinitiation complex (PIC). In human cells, this complex is called SL1 and contains TBP and the TAF 1 s TAF 1 110, TAF 1 63, TAF I 48, and TAF I 41. The TAF 1 equivalents in fission and budding yeast are called Rrn6, Rrn7, and Rrn11, respectively, and are found with TBP in a complex called core factor (1, 2). The minor subunits of SL1 have no equivalents in yeast. RNAPII, finally, transcribes the ribosomal protein-encoding genes (3). RNAPII-dependent transcription also requires a PIC. In a TATA box containing core promoters, the first step in the formation of the PIC is the binding of TBP to the TATA box. TBP binds to the TATA box complexed with TFIID (TAF 11 s) followed by the other GTFs TFIIB, TFIIF, TFIIE, and TFIIH, respectively, to complete PIC formation for the recruitment of pol II (4 -6). However, it is also discussed that a preassembled holoenzyme including pol II, TBP, and the GTFs are targeted with the help of the mediator complex onto the DNA in the core promoter region for PIC formation (7,8).The 141 ribosomal protein genes of fission yeast encoding the full set of 79 ribosomal proteins are TATA-less promoters. Instead, they all contain the highly conserved sequence CAGT-CACA or its inverted form, TGTGACTG, within 100 bp upstream of the ATG start codon. This sequence was termed the HomolD bo...

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Proteomic analysis of the U1 snRNP of Schizosaccharomyces pombe reveals three essential organism-specific proteins

Cited by 13 publications

References 34 publications

The FgSRP1 SR‐protein gene is important for plant infection and pre‐mRNA processing in Fusarium graminearum

The FgSRP1 SR‐protein gene is important for plant infection and pre‐mRNA processing in Fusarium graminearum

The FF domains of yeast U1 snRNP protein Prp40 mediate interactions with Luc7 and Snu71

Rrn7 Protein, an RNA Polymerase I Transcription Factor, Is Required for RNA Polymerase II-dependent Transcription Directed by Core Promoters with a HomolD Box Sequence

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