Proteomic analysis of the soluble and the lysosomal+mitochondrial fractions from rat pancreas: Implications for cerulein-induced acute pancreatitis

García-Hernández, Violeta; Sánchez-Bernal, Carmen; Sarmiento, Nancy; Viana, Rosa; Ferreira, Laura; Pérez, Nieves; Calvo, José F.; Sánchez‐Yagüe, Jesús

doi:10.1016/j.bbapap.2012.06.003

Cited by 10 publications

(9 citation statements)

References 44 publications

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“…We identified 14 down-regulated proteins or isoforms and four up-regulated proteins in HSAP rat models. Serum levels of AAT, an acute-phase reactant previously showed to be elevated in the serum of patients and animals in the early phase of AP 22 23 24 25 26 , were found to be significantly depressed in HSAP rats compared with NHSAP rats. A previous study suggested that AAT has significant anti-inflammatory properties by affecting a wide range of inflammatory cells such as neutrophils, monocytes, macrophages, and mast cells 36 .…”

Section: Discussionmentioning

confidence: 83%

“…2c ). We chose to further investigate the expression of AAT, a 52-kD protein previously involved in the pathology of AP 22 23 24 25 26 .…”

Section: Resultsmentioning

confidence: 99%

“…Imbalance in the synthesis and degradation of proteolytic enzymes and antiproteases has also been implicated in the development of AP. Elevated serum levels of AAT have been detected in AP 23 , and AAT may represent a diagnostic marker of the early phase of AP 24 25 26 . However, the influence of HS on AAT levels in the acute phase of pancreatitis is not known.…”

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confidence: 99%

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Hepatic steatosis depresses alpha-1-antitrypsin levels in human and rat acute pancreatitis

Wang

et al. 2015

Sci Rep

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Hepatic steatosis (HS) can exacerbate acute pancreatitis (AP). This study aimed to investigate the relation between α1-antitrypsin (AAT) and acute pancreatitis when patients have HS. Using proteomic profiling, we identified 18 differently expressed proteins pots in the serum of rats with or without HS after surgical establishment of AP. AAT was found to be one of the significantly down-regulated proteins. AAT levels were significantly lower in hepatic steatosis acute pancreatitis (HSAP) than in non-HSAP (NHSAP) (P < 0.001). To explore the clinical significance of these observations, we measured the levels of AAT in the serum of 240 patients with HSAP, NHSAP, fatty liver disease (FLD), or no disease. Compared with healthy controls, serum AAT levels in patients with NHSAP were significantly higher (P < 0.01), while in patients with HSAP serum AAT levels were significantly lower (P < 0.01). Further studies showed that acute physiology and chronic health evaluation (APACHE-II) scores were negatively correlated with serum AAT levels (r = −0.85, P < 0.01). In conclusion, low serum levels of AAT in patients with HSAP are correlated with disease severity and AAT may represent a potential target for therapies aiming to improve pancreatitis.

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Section: Discussionmentioning

confidence: 83%

“…2c ). We chose to further investigate the expression of AAT, a 52-kD protein previously involved in the pathology of AP 22 23 24 25 26 .…”

Section: Resultsmentioning

confidence: 99%

mentioning

confidence: 99%

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Hepatic steatosis depresses alpha-1-antitrypsin levels in human and rat acute pancreatitis

Wang

et al. 2015

Sci Rep

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“…Fétaud et al [197] concluded that proteomic analysis is a very interesting tool to identify changes characterizing pancreatic tissue damage and new potential biomarkers of AP severity. This method may increase our knowledge of the molecular mechanisms underlying AP and thus enhance new diagnostic and prognostic biomarkers [198,199]. The serum proteomic profile has features that can differentiate mild from severe AP.…”

Section: Proteomic Profilingmentioning

confidence: 99%

Multifactorial Scores and Biomarkers of Prognosis of Acute Pancreatitis: Applications to Research and Practice

Silva-Vaz

Abrantes

Castelo‐Branco

et al. 2020

IJMS

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Acute pancreatitis (AP) is a severe inflammation of the pancreas presented with sudden onset and severe abdominal pain with a high morbidity and mortality rate, if accompanied by severe local and systemic complications. Numerous studies have been published about the pathogenesis of AP; however, the precise mechanism behind this pathology remains unclear. Extensive research conducted over the last decades has demonstrated that the first 24 h after symptom onset are critical for the identification of patients who are at risk of developing complications or death. The identification of these subgroups of patients is crucial in order to start an aggressive approach to prevent mortality. In this sense and to avoid unnecessary overtreatment, thereby reducing the financial implications, the proper identification of mild disease is also important and necessary. A large number of multifactorial scoring systems and biochemical markers are described to predict the severity. Despite recent progress in understanding the pathophysiology of AP, more research is needed to enable a faster and more accurate prediction of severe AP. This review provides an overview of the available multifactorial scoring systems and biochemical markers for predicting severe AP with a special focus on their advantages and limitations.

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“…The isoelectric focusing electrophoresis was performed as previously described (García-Hernández et al 2012). Briefly, 120 g protein was solubilized directly in rehydration buffer, 1% dithiothreitol (DTT), 1% IPG buffer, and 0.002% bromophenol blue, with a total volume of 460 L. Proteins were initially separated using an Ettan IPGphor 3 Isoelectric Focusing System (GE Healthcare, Fairfield, Connecticut, USA).…”

Section: Isoelectric Focusing Electrophoresismentioning

confidence: 99%

Apoptosis of rat hepatic stellate cells induced by diallyl trisulfide and proteomics profiling in vitro

Zhang

Zhou

et al. 2017

Can. J. Physiol. Pharmacol.

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Diallyl trisulfide (DATS), a major garlic derivative, inhibits cell proliferation and triggers apoptosis in a variety of cancer cell lines. However, the effects of DATS on hepatic stellate cells (HSCs) remain unknown. The aim of this study was to analyze the effects of DATS on cell proliferation and apoptosis, as well as the protein expression profile in rat HSCs. Rat HSCs were treated with or without 12 and 24 g/mL DATS for various time intervals. Cell proliferation and apoptosis were determined using tetrazolium dye (MTT) colorimetric assay, bromodeoxyuridine (5-bromo-2=-deoxyuridine; BrdU) assay, Hoechst 33342 staining, electroscopy, and flow cytometry. Protein expression patterns in HSCs were systematically studied using 2-dimensional electrophoresis and mass spectrometry. DATS inhibited cell proliferation and induced apoptosis of HSCs in a time-dependent manner. We observed clear morphological changes in apoptotic HSCs and dramatically increased annexin V-positive -propidium iodide negative apoptosis compared with the untreated control group. Twenty-one significant differentially expressed proteins, including 9 downregulated proteins and 12 upregulated proteins, were identified after DATS administration, and most of them were involved in apoptosis. Our results suggest that DATS is an inducer of apoptosis in HSCs, and several key proteins may be involved in the molecular mechanism of apoptosis induced by DATS.Key words: diallyl trisulfide, apoptosis, hepatic stellate cells, differentially expressed proteins.Résumé : Le trisulfure de diallyle (TSDA), un dérivé important de l'ail, inhibe la prolifération cellulaire et déclenche l'apoptose dans une variété de lignées cellulaires cancéreuses. Cependant, les effets du TSDA sur les cellules de Kupffer (CK) restent inconnus. Cette étude visait à analyser les effets du TSDA sur la prolifération cellulaire et l'apoptose, de même que sur le profil d'expression des protéines dans les CK de rat. Les CK de rat ont été traitées ou non avec 12 et 24 g/mL de TSDA pendant différentes périodes de temps. La prolifération cellulaire et l'apoptose ont été déterminées à l'aide d'un dosage colorimétrique au tétrazolium (MTT), d'un dosage à la bromodéoxyuridine (5-bromo-2=-déoxyuridine, BrdU), par coloration au Hoechst 33342, par microscopie électronique et par cytométrie en flux. Les patrons d'expression protéique des CK ont été systématiquement étudiés par électrophorèse en deux dimensions et par spectroscopie de masse. Le TSDA inhibait la prolifération cellulaire et induisait l'apoptose des CK en fonction du temps. Les auteurs ont observé des changements morphologiques clairs des CK en apoptose et une augmentation importante des cellules en apoptose positives à l'annexine V et négatives à l'iodure de propidium comparativement au groupe contrôle non traité. Vingt et une protéines exprimées de façon différentielle, soit neuf protéines régulées à la baisse et 12 protéines régulées à la hausse, ont été identifiées à la suite de l'administration de TSDA, et la plupart d'ent...

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Proteomic analysis of the soluble and the lysosomal+mitochondrial fractions from rat pancreas: Implications for cerulein-induced acute pancreatitis

Cited by 10 publications

References 44 publications

Hepatic steatosis depresses alpha-1-antitrypsin levels in human and rat acute pancreatitis

Hepatic steatosis depresses alpha-1-antitrypsin levels in human and rat acute pancreatitis

Multifactorial Scores and Biomarkers of Prognosis of Acute Pancreatitis: Applications to Research and Practice

Apoptosis of rat hepatic stellate cells induced by diallyl trisulfide and proteomics profiling in vitro

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