Proteomic Analysis of the Rat Ovary Following Chronic Low-Dose Exposure to 2,3,7,8-Tetrachlorodibenzo-<i>p</i>-Dioxin (TCDD)

Chen, Xi; Ma, Xiaoming; Ma, Shiwei; Coenraads, Pieter Jan; Zhang, Chunmei; Liu, Jing; Zhao, Lijun; Sun, Min; Tang, Naijun

doi:10.1080/15287390902841136

Cited by 16 publications

(5 citation statements)

References 56 publications

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“…Consistent with this, female mice exposed to TCDD exhibited low levels of estradiol ( Huang et al, 2011 ). A similar scenario was observed in Sprague-Dawley rats after oral administration of TCDD for a long period ( Chen et al, 2009 ). Other studies have revealed that TCDD inhibits estrogenic signaling in the kidney and liver but paradoxically promotes estrogenic signaling in the pituitary gland within the same individual ( Yoshida et al, 2020 ).…”

Section: Dioxins and Their Sourcessupporting

confidence: 73%

Impact of dioxins on reproductive health in female mammals

Aldeli,

Murphy,

Hanano

2024

Front. Toxicol.

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Extensive research has been conducted to investigate the toxicological impact of dioxins on mammals, revealing profound effects on the female reproductive system in both humans and animals. Dioxin exposure significantly disrupts the intricate functions of the ovary, a pivotal organ responsible for reproductive and endocrine processes. This disruption manifests as infertility, premature ovarian failure, and disturbances in sex steroid hormone levels. Comprehensive studies, encompassing accidental human exposure and experimental animal data, have raised a wealth of information with consistent yet varied conclusion influenced by experimental factors. This review begins by providing an overarching background on the ovary, emphasizing its fundamental role in reproductive health, particularly in ovarian steroidogenesis and hormone receptor regulation. Subsequently, a detailed examination of the Aryl hydrocarbon Receptor (AhR) and its role in governing ovarian function is presented. The review then outlines the sources and toxicity of dioxins, with a specific focus on AhR involvement in mediating reproductive toxicity in mammals. Within this context, the impact of dioxins, notably 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD), on Folliculogenesis and Preimplantation embryos is discussed. Furthermore, the review delves into the disruptions of the female hormonal system caused by TCDD and their ramifications in endometriosis. Notably, variations in the effects of TCDD on the female reproductive and hormonal system are highlighted in relation to TCDD dose, animal species, and age. As a forward-looking perspective, questions arise regarding the potential involvement of molecular mechanisms beyond AhR in mediating the female reproductive toxicity of dioxins.

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Section: Dioxins and Their Sourcessupporting

confidence: 73%

Impact of dioxins on reproductive health in female mammals

Aldeli,

Murphy,

Hanano

2024

Front. Toxicol.

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“…In parallel, recent studies observed that environmental toxicants adversely affect ovarian function and fertility 19,20 . Chen et al 21 performed proteomic analysis of the ovaries by two-dimensional gel electrophoresis and matrix-assisted laser desorption/ionization tandem mass spectrometry and showed distinct changes in the levels of several proteins that are relevant markers of TCDD toxicity. Valdez et al 22 revealed that TCDD exposure cause the failure of ovarian function via alteration of ovarian gene expression.…”

Section: Tcdd Toxicity On Uterine and Ovarian Tissuesmentioning

confidence: 99%

The beneficial effects of Montelukast against 2,3,7,8-tetrachlorodibenzo- p -dioxin toxicity in female reproductive system in rats

et al. 2016

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PURPOSE:To determine the toxic effect of 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) on reproductive system and the beneficial effects of Montelukast (ML) with histological and biochemical analysis. METHODS:Rats were randomly divided into four equal groups (control, TCDD, ML and TCDD+ML). Tissue samples were collected on day 60 and oxidative status and histological alterations were analyzed. RESULTS:The results showed a significant increase in oxidative and histological damage on uterine and ovarian tissues. Otherwise, the oxidative and histological damages caused by TCDD were prevented with ML treatment. CONCLUSION:The toxic effects of 2,3,7,8-tetrachlorodibenzo-p-dioxin on female reproductive system were reversed with Montelukast treatment. Therefore, we claimed that ML treatment might be useful for TCDD toxicity.

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“…This compound induces an endocrine disruption that affects the reproductive system. In this study, the matrix-assisted laser desorption/ionization (MALDI) tandem mass spectrometry showed distinct changes in the levels of several proteins that are relevant markers for TCDD toxicity [ 45 ]. Proteomics is also useful to evaluate protein profiles from cell cultures.…”

Section: “Omics” In Analysis Of Chemical Exposurementioning

confidence: 99%

The Role of Molecular Biology in the Biomonitoring of Human Exposure to Chemicals

Muñoz

Albores

2010

IJMS

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Exposure to different substances in an occupational environment is of utmost concern to global agencies such as the World Health Organization and the International Labour Organization. Interest in improving work health conditions, particularly of those employees exposed to noxious chemicals, has increased considerably and has stimulated the search for new, more specific and selective tests. Recently, the field of molecular biology has been indicated as an alternative technique for monitoring personnel while evaluating work-related pathologies. Originally, occupational exposure to environmental toxicants was assessed using biochemical techniques to determine the presence of higher concentrations of toxic compounds in blood, urine, or other fluids or tissues; results were used to evaluate potential health risk. However, this approach only estimates the presence of a noxious chemical and its effects, but does not prevent or diminish the risk. Molecular biology methods have become very useful in occupational medicine to provide more accurate and opportune diagnostics. In this review, we discuss the role of the following common techniques: (1) Use of cell cultures; (2) evaluation of gene expression; (3) the “omic” sciences (genomics, transcriptomics, proteomics and metabolomics) and (4) bioinformatics. We suggest that molecular biology has many applications in occupational health where the data can be applied to general environmental conditions.

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Proteomic Analysis of the Rat Ovary Following Chronic Low-Dose Exposure to 2,3,7,8-Tetrachlorodibenzo-p-Dioxin (TCDD)

Cited by 16 publications

References 56 publications

Impact of dioxins on reproductive health in female mammals

Impact of dioxins on reproductive health in female mammals

The beneficial effects of Montelukast against 2,3,7,8-tetrachlorodibenzo- p -dioxin toxicity in female reproductive system in rats

The Role of Molecular Biology in the Biomonitoring of Human Exposure to Chemicals

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