Proteomic analysis of the marine-derived fungus Paecilomyces sp. strain SF-8 in response to polycyclic aromatic hydrocarbons

Velmurugan, Natarajan; Lee, Hyun-Mi; Cha, Hyojung; Lee, Yang-Soo

doi:10.1515/bot-2016-0101

Cited by 11 publications

(11 citation statements)

References 31 publications

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“…This observation is in accordance with studies reporting that reactive oxygen species produced by PAH metabolism cause lipid peroxidative injury (Singh et al, 2007;Gao et al, 2019). Our results are in agreement with studies performed in other fungi, reporting that genes mediating repair of oxidative damage were upregulated during PAH biodepletion (Thuillier et al, 2014;Velmurugan et al, 2017;Camacho-Morales et al, 2018; (Loss et al, 2019) and Aspergillus sydowii (this study) were included in the analysis. [Color figure can be viewed at wileyonlinelibrary.com] Loss et al, 2019).…”

Section: Detoxification and Self-defence Mechanisms Upon Polyaromatic Exposuresupporting

confidence: 93%

“…is not the only ascomycetous genus with an outstanding capability to remove a wide range of highly cytotoxic PAHs. Other ascomycetes such as Fusarium equiseti, Paecilomyces sp., Penicillium oxalicum, Penicillium pinophilum, Pestalotiopsis sp., Pseudollascheria boydii, Talaromyces verruculosus, Trichoderma asperellum and Trichoderma harzianum have also shown a versatile ability to degrade PAHs (Kristanti and Hadibarata, 2015; Zafra et al ., 2016; Aranda et al ., 2017; Velmurugan et al ., 2017; Gao et al ., 2019).…”

Section: Discussionmentioning

confidence: 99%

“…Reactive intermediates produced during Phe and BaP biodegradation can covalently bind DNA forming PAH‐DNA adducts, which increase the likelihood of gene mutations. Moreover, BaP induces genomic methylation disrupting the DNA methylation patterns in eukaryotes (Velmurugan et al ., 2017). The A. sydowii transcriptomic analysis revealed an upregulation of certain genes involved in the DNA repair mechanisms, genome integrity and the expression and stability of mRNA.…”

Section: Discussionmentioning

confidence: 99%

“…DNA damage responses were upregulated also in PAH‐treated mycelium of A. nidulans, Paecilomyces sp. and P. oxalicum (Velmurugan et al ., 2017; Camacho‐Morales et al ., 2018; Loss et al ., 2019). In addition, Phe and BaP affected in A. sydowii the nuclear trafficking of proteins and mRNA since trp genes were overexpressed.…”

Section: Discussionmentioning

confidence: 99%

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Transcriptomic analysis of polyaromatic hydrocarbon degradation by the halophilic fungus Aspergillus sydowii at hypersaline conditions

Peidro-Guzmán

Pérez-Llano

González-Abradelo

et al. 2020

Environmental Microbiology

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Polycyclic aromatic hydrocarbons (PAHs) are among the most persistent xenobiotic compounds, with high toxicity effects. Mycoremediation with halophilic Aspergillus sydowii was used for their removal from a hypersaline medium (1 M NaCl). A. sydowii metabolized PAHs as sole carbon sources, resulting in the removal of up to 90% for both PAHs [benzo [a] pyrene (BaP) and phenanthrene (Phe)] after 10 days. Elimination of Phe and BaP was almost exclusively due to biotransformation and not adsorption by dead mycelium and did not correlate with the activity of lignin modifying enzymes (LME). Transcriptomes of A. sydowii grown on PAHs, or on glucose as control, both at hypersaline conditions, revealed 170 upregulated and 76 downregulated genes. Upregulated genes were related to starvation, cell wall remodelling, degradation and metabolism of xenobiotics, DNA/RNA metabolism, energy generation, signalling and general stress responses. Changes of LME expression levels were not detected, while the chloroperoxidase gene, possibly related to detoxification processes in fungi, was strongly upregulated. We propose that two parallel metabolic pathways (mitochondrial and cytosolic) are involved in degradation and detoxification of PAHs in A. sydowii resulting in intracellular oxidation of PAHs. To the best of our knowledge, this is the most comprehensive transcriptomic analysis on fungal degradation of PAHs.

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Section: Detoxification and Self-defence Mechanisms Upon Polyaromatic Exposuresupporting

confidence: 93%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

See 2 more Smart Citations

Transcriptomic analysis of polyaromatic hydrocarbon degradation by the halophilic fungus Aspergillus sydowii at hypersaline conditions

Peidro-Guzmán

Pérez-Llano

González-Abradelo

et al. 2020

Environmental Microbiology

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show abstract

“…The inclusion of a proteomic approach helps to identify related enzymes and their metabolic pathways in the bioremediation of xenobiotics from various contaminated sites (Kim et al, 2004 ; Liu et al, 2017 ; Wei et al, 2017 ). Basically, there are four primary steps that involve proteomic analysis: (1) preparation of a biological sample; (2) extraction and separation of proteins by using two-dimensional gel electrophoresis (2D-GE); (3) protein gel images are examined by means of image-analysis software such as ImageMaster 2D or PDQuest; and (4) proteins are identified by using mass spectroscopy (MS)/MALDI-TOF/MS or LC-MS (Yates et al, 2009 ; Chakka et al, 2015 ; Velmurgan et al, 2017 ).…”

Section: Recent Advanced Technologies Employed In Bioremediation For mentioning

confidence: 99%

Recent Advanced Technologies for the Characterization of Xenobiotic-Degrading Microorganisms and Microbial Communities

Mishra

Lin

Pang

et al. 2021

Front. Bioeng. Biotechnol.

181

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Global environmental contamination with a complex mixture of xenobiotics has become a major environmental issue worldwide. Many xenobiotic compounds severely impact the environment due to their high toxicity, prolonged persistence, and limited biodegradability. Microbial-assisted degradation of xenobiotic compounds is considered to be the most effective and beneficial approach. Microorganisms have remarkable catabolic potential, with genes, enzymes, and degradation pathways implicated in the process of biodegradation. A number of microbes, including Alcaligenes, Cellulosimicrobium, Microbacterium, Micrococcus, Methanospirillum, Aeromonas, Sphingobium, Flavobacterium, Rhodococcus, Aspergillus, Penecillium, Trichoderma, Streptomyces, Rhodotorula, Candida, and Aureobasidium, have been isolated and characterized, and have shown exceptional biodegradation potential for a variety of xenobiotic contaminants from soil/water environments. Microorganisms potentially utilize xenobiotic contaminants as carbon or nitrogen sources to sustain their growth and metabolic activities. Diverse microbial populations survive in harsh contaminated environments, exhibiting a significant biodegradation potential to degrade and transform pollutants. However, the study of such microbial populations requires a more advanced and multifaceted approach. Currently, multiple advanced approaches, including metagenomics, proteomics, transcriptomics, and metabolomics, are successfully employed for the characterization of pollutant-degrading microorganisms, their metabolic machinery, novel proteins, and catabolic genes involved in the degradation process. These technologies are highly sophisticated, and efficient for obtaining information about the genetic diversity and community structures of microorganisms. Advanced molecular technologies used for the characterization of complex microbial communities give an in-depth understanding of their structural and functional aspects, and help to resolve issues related to the biodegradation potential of microorganisms. This review article discusses the biodegradation potential of microorganisms and provides insights into recent advances and omics approaches employed for the specific characterization of xenobiotic-degrading microorganisms from contaminated environments.

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Bioinformatics Toward Improving Bioremediation

Khanna

Kumar

2022

Biotechnological Innovations for Environmental Bioremediation

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Proteomic analysis of the marine-derived fungus Paecilomyces sp. strain SF-8 in response to polycyclic aromatic hydrocarbons

Abstract: An efficient and unique proteomic response is important for fungi to survive in marine environments polluted with polycyclic aromatic hydrocarbons (PAHs). The marine-derived fungus

Cited by 11 publications

References 31 publications

Transcriptomic analysis of polyaromatic hydrocarbon degradation by the halophilic fungus Aspergillus sydowii at hypersaline conditions

Transcriptomic analysis of polyaromatic hydrocarbon degradation by the halophilic fungus Aspergillus sydowii at hypersaline conditions

Recent Advanced Technologies for the Characterization of Xenobiotic-Degrading Microorganisms and Microbial Communities

Bioinformatics Toward Improving Bioremediation

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