Proteomic analysis of the key mechanism of exocarp russet pigmentation of semi-russet pear under rainwater condition

Shi, Chunhui; Qi, Baoxiu; Wang, Xiaoqing; Lianying, Shen; Jun, Luo; Zhang, Yuxing

doi:10.1016/j.scienta.2019.04.086

Cited by 24 publications

(22 citation statements)

References 35 publications

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“…Russet in ‘Apple’ mango is similar to russet phenomena in other fruitcrop species, such as Malus apple [ 15 , 16 ], pear [ 17 ], citrus [ 18 ], grape [ 8 ], and melon [ 19 ]. This conclusion is based on the following arguments: (1) the appearance of the disorder in ‘Apple’ mango with rough brownish irregular patches surrounded by interconnected light brownish cracks is identical to that previously described for Malus apple [ 7 ].…”

Section: Discussionmentioning

confidence: 99%

Russeting in ‘Apple’ Mango: Triggers and Mechanisms

Athoo

Winkler

Knoche

2020

Plants

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Russeting is an important surface disorder of many fruitcrop species. The mango cultivar ‘Apple’ is especially susceptible to russeting. Russeting compromises both fruit appearance and postharvest performance. The objective was to identify factors, mechanisms, and consequences of russeting in ‘Apple’ mango. Russeting was quantified on excised peels using image analysis and a categorical rating scheme. Water vapour loss was determined gravimetrically. The percentage of the skin area exhibiting russet increased during development. Russet began at lenticels then spread across the surface, ultimately forming a network of rough, brown patches over the skin. Cross-sections revealed stacks of phellem cells, typical of a periderm. Russet was more severe on the dorsal surface of the fruit than on the ventral and more for fruit in the upper part of the canopy than in the lower. Russet differed markedly across orchards sites of different climates. Russet was positively correlated with altitude, the number of rainy days, and the number of cold nights but negatively correlated with minimum, maximum, and mean daily temperatures, dew point temperature, and heat sum. Russeted fruit had higher transpiration rates than non-russeted fruits and higher skin permeance to water vapour. Russet in ‘Apple’ mango is due to periderm formation that is initiated at lenticels. Growing conditions conducive for surface wetness exacerbate russeting.

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Section: Discussionmentioning

confidence: 99%

Russeting in ‘Apple’ Mango: Triggers and Mechanisms

Athoo

Winkler

Knoche

2020

Plants

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“…The fruit bag is supposed to prevent rainwater and change the light . Shi et al (2019) revealed that the rain shelter might affect phenylalanine lignin monomer synthesis and decreased peel russet accumulation in semi-russet pear. Previous research has largely focused on uncovering the physiological, biochemical, and molecular mechanisms responsible for russeting; however, the way in which light quality influences this process in sand pear is largely unknown.…”

Section: Discussionmentioning

confidence: 99%

“…The suberification process is closely related to lignin and phenolic concentrations (Bernards, 2002;Grac xa and Santos, 2007), and it has been reported that lignin, cellulose, and hemicellulose contents are higher in russet peel than in green peel (Heng et al, 2014;Shi et al, 2019). Suberin is a macromolecular structure composed of the suberin poly-phenolic domain (SPPD) and the suberin polyaliphatic domain (SPAD), which are covalently linked by hydroxyferulic acid and its derivatives in a lignin-like manner (Meyer et al, 2011a(Meyer et al, , 2011b.…”

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confidence: 99%

“…Numerous enzymes are involved in the synthesis of lignin, including phenylalanine ammonia-lyase (PAL), 4-coumaric acid coenzyme A ligase (4CL), cinnamyl alcohol dehydrogenase (CAD), cinnamate-4-hydroxylase (C4H), cinnamoyl-CoA reductase (CCR), and peroxidase (POD) (Hu et al, 2017;Mertz-Henning et al, 2015;Shi et al, 2019). Most of the enzymes in the lignin metabolic pathway are induced by light at the transcriptional level, such as PAL, the activity of which is closely related to C4H, 4CL, CAD, and CCR (Ferrer et al, 2008;Hemm et al, 2004).…”

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confidence: 99%

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Response of Fruit Bagging to Lignin Biosynthesis and Expression of Related Genes in Fruit Peel of Sand Pear (Pyrus pyrifolia Nakai) cv. Cuiguan

Shi¹,

Wang²,

Zhang³

et al. 2019

horts

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This study explored the effects of different colored bags (blue, green, white, yellow, orange, and red) on russet deposition on the peel of semi-russet ‘Cuiguan’ pears 10 days after full bloom (DAFB). The process of russeting of the peel and structure of the cork layer were characterized by microscopy and scanning electron microscopy (SEM), followed by the detection of lignin and the activity of enzymes involved in lignin synthesis. The expression of cinnamate-4-hydroxylase, 4-coumarate:coenzyme A ligase, cinnamyl alcohol dehydrogenase, cinnamoyl-CoA reductase, and peroxidase, which were related to phenylalanine ammonia-lyase, was determined via real-time quantitative polymerase chain reaction. Russeting of the outer peel of ‘Cuiguan’ pear accumulated rapidly at 80 DAFB, and a positive relationship between the russet index and lignin content was observed. Red and infrared (IR) ray, partial far-IR light (600–800 nm), and ultraviolet-A light (350–400 nm) promoted russeting in ‘Cuiguan’ pear peel, whereas green light decreased russeting, the russet index, enzymatic activities, and the expression levels of enzymes involved in lignin synthesis. Values of all these factors were higher for ‘Cuiguan’ pears in red bags than for those in bags of other colors. These findings suggested that spectral components affected the synthesis of lignin and the formation of fruit russet. Storage in green bags reduced russeting and improved fruit appearance.

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“…Emerging evidence has indicated that heavy rainfall may be one of the most important factors inducing suberin accumulation (Lara et al , 2015). In preliminary studies, we observed a sharp increase in lignin, the main compound comprising the russet exocarp under excessive rainfall (Shi et al, 2019a). This affects phenylalanine lignin monomer synthesis and defense metabolism by regulating the corresponding proteins.…”

Section: Introductionmentioning

confidence: 66%

Network of multi-omics reveals the mechanism of russeting under rainwater in pears

Shi

Wang

Zhang

et al. 2020

Preprint

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Russeting occurs via the epidermal suberin accumulation model in the phenol-ester protection system in pears, especially under excessive rainfall. To clarify the role of rain in the russeting mechanism, we applied multi-omics (transcriptome, metabolome, and proteome) integrated analysis from three colored pear cultivars grown under two rainfall conditions, including a no rain control (C) and a rain-abundant (R) treatment. Broad alterations occurred in 29 overlapping differentially expressed genes (DEGs) involved in the response to stress and secondary metabolite biosynthesis. Three overlapping differentially expressed proteins (DEPs) and seven differentially expressed metabolites (DEMs) were identified among the three comparison groups.Cross-comparison of mRNA and protein data revealed co-expression of DEGs and DEPs. Suberin and phenylpropane were markedly enhanced, while cutin biosynthesis and fatty acid elongation were depressed in russet pears under R. In russet pears, the triacontane associated with wax composition was abruptly missing. Moreover, The PpyHHT1 (103966555) gene and encoded protein HHT1 (694406379), which acts as a 'bridge' between phenol-and ester-polymerization, had significantly upregulated expression. Collectively, we provide a comprehensive model of the molecular russeting mechanism, which provides powerful insight into russeting and shows the plasticity of plant defenses to cope with the harsh natural environment.

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Proteomic analysis of the key mechanism of exocarp russet pigmentation of semi-russet pear under rainwater condition

Cited by 24 publications

References 35 publications

Russeting in ‘Apple’ Mango: Triggers and Mechanisms

Russeting in ‘Apple’ Mango: Triggers and Mechanisms

Response of Fruit Bagging to Lignin Biosynthesis and Expression of Related Genes in Fruit Peel of Sand Pear (Pyrus pyrifolia Nakai) cv. Cuiguan

Network of multi-omics reveals the mechanism of russeting under rainwater in pears

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