Proteomic analysis of the excretory-secretory products from Strongyloides venezuelensis infective larvae: new insights for the immunodiagnosis of human strongyloidiasis

Roldán, William H.; Coelho, Guilherme Rabelo; Pimenta, Daniel Carvalho; Paula, Fabiana Martins de; Gryschek, Ronaldo Cesar Borges

doi:10.1007/s00436-022-07636-y

Cited by 1 publication

(5 citation statements)

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“…Indeed, ESPs are obtained through the maintenance of larvae in vitro, and different maintenance conditions—in terms of incubation time, medium and larval density—have been reported in the literature. This technical variability seems to affect the larval metabolic state and, as a consequence, the ESP pattern [58] since differences in ESP proteomics composition were observed in larvae maintained in PBS or RPMI [27]. The identification of immunoreactive proteins thus seems essential to overcome this variability, as was done in iL3-ESP from S. venezuelensis.…”

Section: Immunoproteomicsmentioning

confidence: 99%

“…The identification of immunoreactive proteins thus seems essential to overcome this variability, as was done in iL3-ESP from S. venezuelensis. Mass spectrometry in fact identified arginine kinase as a protein band that is highly immunoreactive with patients' serum (93% sensitivity and 100% specificity) and that has no cross-reactivity with serum samples from patients with other helminthiases [27].…”

Section: Immunoproteomicsmentioning

confidence: 99%

“…Despite their potential, the study of helminth-derived EVs presents numerous challenges and a standardization of the working procedures was recently deemed necessary, prompting the publication of recommended guidelines for their study [55]. Although experimental data documenting Strongyloides-derived EVs are not yet available, Gonzales et al employed bioinformatics tools to predict the presence of numerous proteins secreted within EVs among those identified in S. venezuelensis iL3 ESPs [27], concluding that EVs release is also likely to occur in Strongyloides.…”

Section: Proteomics Analysis Of Strongyloides Spp Excretory/secretory...mentioning

confidence: 99%

“…Both these proteins have already been identified in multiple studies as candidate diagnostic markers both in Strongyloides spp. as well as in other parasitic helminths [17,27,28]. Moreover, enolase has already been proposed as a vaccine candidate against Ascaris suum [62], while galectins are involved in the initiation of the host immune response [35,[63][64][65].…”

Section: Immunoproteomicsmentioning

confidence: 99%

“…Although experimental data documenting Strongyloides -derived EVs are not yet available, Gonzales et al . employed bioinformatics tools to predict the presence of numerous proteins secreted within EVs among those identified in S. venezuelensis iL3 ESPs [27], concluding that EVs release is also likely to occur in Strongyloides .…”

Section: Proteomics Analysis Of Strongyloides Spp Excretory/secretory...mentioning

confidence: 99%

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Progresses and challenges in Strongyloides spp. proteomics

Tiberti,

Manfredi,

Piubelli

et al. 2023

Phil. Trans. R. Soc. B

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The availability of high-quality data of helminth genomes provided over the past two decades has supported and accelerated large-scale ‘omics studies and, consequently, the achievement of a more in-depth molecular characterization of a number of pathogens. This has also involved Strongyloides spp. and since their genome was made available transcriptomics has been rather frequently applied to investigate gene expression regulation across their life cycle. Strongyloides proteomics characterization has instead been somehow neglected, with only a few reports performing high-throughput or targeted analyses associated with protein identification by tandem mass spectrometry. Such investigations are however necessary in order to discern important aspects associated with human strongyloidiasis, including understanding parasite biology and the mechanisms of host–parasite interaction, but also to identify novel diagnostic and therapeutic targets. In this review article, we will give an overview of the published proteomics studies investigating strongyloidiasis at different levels, spanning from the characterization of the somatic proteome and excretory/secretory products of different parasite stages to the investigation of potentially immunogenic proteins. Moreover, in the effort to try to start filling the current gap in host-proteomics, we will also present the first serum proteomics analysis in patients suffering from human strongyloidiasis. This article is part of the Theo Murphy meeting issue ‘ Strongyloides : omics to worm-free populations’.

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Section: Immunoproteomicsmentioning

confidence: 99%

Section: Immunoproteomicsmentioning

confidence: 99%